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Cloning and characteristics of a novel gene HbUEP from latex in Hevea brasiliensis

Published online by Cambridge University Press:  01 October 2008

Yang Yun ,
Zhang Zhi-Li ,
Liu Kuan-Can ,
Li Wei-Guo  and
Su Huo-Sheng
Yang Yun
Affiliation:
Institute of Tropical Biosciences and Biotechnology, CATAS, Haikou 571101, China Institute of Medicinal Plant Development, Hainan Branch, Wanning 571500, China
Zhang Zhi-Li*
Affiliation:
Institute of Tropical Biosciences and Biotechnology, CATAS, Haikou 571101, China
Liu Kuan-Can
Affiliation:
Institute of Tropical Biosciences and Biotechnology, CATAS, Haikou 571101, China
Li Wei-Guo
Affiliation:
Institute of Rubber Research, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China
Su Huo-Sheng
Affiliation:
Institute of Tropical Biosciences and Biotechnology, CATAS, Haikou 571101, China
*
*Corresponding author. E-mail: [email protected]
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Abstract

HbUEP, an ubiquitin extension protein gene from latex of the rubber tree (Hevea brasiliensis) was cloned and sequenced using a differentially ethphon-induced expressed cDNA subtraction library. The cDNA had 771 bp nucleotides, comprising a 226 bp 3′ untranslated region (UTR), 77 bp 5′UTR and a 468 bp open reading frame encoding a 156 amino acid peptide. Southern blotting analysis showed that this gene was a low copy number gene in the H. brasiliensis genome. Within 24 h after application of ethphon, the gene was expressed weakly in both control and latex sampled at 6 h, and strongly in latex sampled at 12 h, showing that this gene expression could be regulated by ethphon. Ethphon could increase the latex yield in H. brasiliensis. It is suggested that the HbUEP gene may be involved in the regulation of ethphon-induced high latex yield in H. brasiliensis.

Keywords

Hevea brasiliensisethphon-induced latexHbUEP geneexpression
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 5 , Issue 2 , August 2008 , pp. 165 - 168
Copyright
Copyright © China Agricultural University 2008

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Footnotes

First published in Journal of Agricultural Biotechnology 2008, 16(2): 305–308

References

Callis, J, Rassch, JA and Vierstra, RD (1990) Ubiquitin extension protein of Arabidopsis thaliana. Journal of Biological Chemistry 265: 12486–12493.CrossRefGoogle ScholarPubMed
Frugis, G and Chua, NH (2002) Ubiquitin-mediated proteolysis in plant hormone signal transduction. Trends in Cell Biology 12(7): 308–311.CrossRefGoogle ScholarPubMed
Garbarino, JE and Belknap, WR (1994) Isolation of a ubiquitin-ribosomal protein gene from potato and expression of its promoter in transgenic plants. Plant Molecular Biology 24: 119–127.CrossRefGoogle ScholarPubMed
Guo, H and Ecker, JR (2003) Plant responses to ethylene gas are mediated by SCF EBF1/EBF2-dependent proteolysis of EIN3 transcription factor. Cell 115: 667–677.CrossRefGoogle Scholar
Huang, GX, Wu, KX and Chen, SC (2002) An improved method for the isolation of total RNA from latex in Hevea brasiliensis. Journal of South China University of Tropical Agriculture 8(4): 1–4 (in Chinese with English abstract).Google Scholar
Peng, SQ and Chen, SC (2002) Expression analysis of polyubiquitin gene in Hevea brasiliensis. Chinese Journal of Tropical Crops 23(3): 32–35 (in Chinese with English abstract).Google Scholar
Potuschak, T, Lechner, E, Parmentier, Y, Yanagisawa, S, Grava, S, Koncz, C and Genschik, P (2003) EIN3-dependent regulation of plant ethylene hormone signaling by two Arabidopsis F box proteins: EBF1 and EBF2. Cell 115: 679–689.CrossRefGoogle ScholarPubMed
Vierstra, RD (2003) The ubiquitin/26S proteasome pathway, the complex last chapter in the life of many plant proteins. Trends in Plant Science 8(3): 135–142.CrossRefGoogle ScholarPubMed
Zhang, ZL, Yang, Y, Liu, KC and Su, HS (2007) A rapid and efficient protocol for total RNA isolation from latex of Hevea brasiliensis. Chinese Bulletin of Botany 24(4): 516–520 (in Chinese with English abstract).Google Scholar