Hostname: page-component-cd9895bd7-p9bg8 Total loading time: 0 Render date: 2024-12-18T16:46:59.228Z Has data issue: false hasContentIssue false
  • English
  • Français

Biological characteristics of recombinant enhancin of Trichoplusia ni granulovirus expressed in Escherichia coli

Published online by Cambridge University Press:  20 March 2007

Yuan Zhe-Ming ,
Meng Xiao-Lin  and
Liu Shu-Sheng
Yuan Zhe-Ming
Affiliation:
Bio-safety Science and Technology College, Hunan Agricultural University, Changsha 410128, China
Meng Xiao-Lin*
Affiliation:
Institute of Virology, Wuhan University, Wuhan 430072, China
Liu Shu-Sheng
Affiliation:
Institute of Applied Entomology, Zhejiang University, Hangzhou 310029, China
*
*Corresponding author. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

A recombinant enhancin from Trichoplusia ni granulovirus, expressed in Escherichia coli and named P96, had been shown in a previous study to increase significantly the efficacy of bio-insecticides applied to insect pests. Here, several biological characteristics were clarified to provide further proof for the activities of P96. The inclusion bodies formed by P96 were quite stable when expressed in E. coli; however, they could be effectively dissolved in sodium carbonate (Na2CO3) solution or in the midgut digestive secretion of the fifth-instar larvae of the cotton bollworm, Helicoverpa armigera, in vitro. According to sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE), the peritrophic membrane of the cotton bollworm was clearly decomposed by P96. With the increase of P96 concentration, the mortality of the cotton bollworm caused by nuclear polyhedrosis virus increased up to its saturation concentration, 6.76×105 inclusion bodies/ml. The bioactivity of P96 was strongly inhibited by ethylenediaminetetraacetic acid (EDTA), which formed a complex compound with the metal ion (Zn2+) in the protein.

Keywords

biological charactersHelicoverpa armigerarecombinant enhancinTrichoplusia ni granulovirus
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 3 , Issue 3 , December 2006 , pp. 171 - 176
Copyright
China Agricultural University and Cambridge University Press 2006

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bischoff, DS and Slavicek, JM (1997) Molecular analysis of an enhancing gene in the Lymantria dispar nuclear polyhedrosis virus. Journal of Virology 71(11): 8133–8140.CrossRefGoogle ScholarPubMed
Chen, HT, Pan, XY and Yuan, ZM (2003) The debilitation of recombinant enhancin on cotton bollworm. Journal of Hunan Agricultural University 29(4): 308–310 (in Chinese with English abstract).Google Scholar
Derksen, ACG and Granados, RR (1988). Alteration of lepidopteran peritrophic membrane by baculoviruses and enhancement of viral infectivity. Virology 167(1): 242–250.CrossRefGoogle ScholarPubMed
Dong, LQ, Zhang, KQ and Qiu, BS (2003) Evaluation of the feasibility of using lactose as the inducer in producing the viral enhancing factor from Helicoverpa armigera granulovirus in Escherichia coli BL21DE3. Microbiology 30(6): 5–9 (in Chinese with English abstract).Google Scholar
El-Moursy, A, Aboul-Ela, R and Salama, HS (1992) Chemical additives that affect the potency of endotoxin of Bacillus thuringiensis against Plodia interpunctella. Insect Science Applied 15(6): 775–779.Google Scholar
Fang, M and Huang, H-L (2001) Advances in in vitro refolding of inclusion body proteins. Chinese Journal of Biotechnology 17(6): 608–612 (in Chinese with English abstract).Google ScholarPubMed
Hashimoto, Y, Corsaro, BG and Granaos, RR (1991) Location and nuleotide sequence of the gene encoding the viral enhancing factor of the Trichoplusia ni granulosis virus. Journal of General Virology 72(11): 2645–2651.CrossRefGoogle Scholar
Hu, R, Meng, XL and Xu, JP (2001) Expression of 2.6 kb enhancin gene fragment from Helicoverpa armigera granulovirus. Virologica Sinica 16(4): 364–368 (in Chinese with English abstract).Google Scholar
Liao, M-D, Xie, Q-L and Lin, J (2002) Heterologous gene high expression in Escherichia coli. Chinese Bulletin of Life Science 14(5): 283–287 (in Chinese with English abstract).Google Scholar
Liu, XG, Yang, G and Qiu, BS (2000) Molecular cloning of enhancin gene from Helicoverpa armigera granulovirus and its expression in E. coli. Acta Microbiologica Sinica 40(4): 379–383 (in Chinese with English abstract).Google Scholar
Liu, XG, Yang, G and Qiu, BS (2001). Expression of two truncated enhancin genes from Helicoverpa armigera granulovirus in E. coli and its preliminary bioassay. Acta Microbiologica Sinica 41(2): 168–172 (in Chinese with English abstract).Google Scholar
Ou, Y, Meng, XL and Xu, JP (2000) Expression of 2.1 kb enhancin gene fragment from Helicoverpa armigera granulovirus in Escherichia coli. Chinese Journal of Biotechnology 16(5): 595–598 (in Chinese with English abstract).Google Scholar
Roelvink, PW, Corsaro, BG and Granados, RR (1995) Characterization of the Helicoverpa armigera and Pseudaletia unipuncta granulovirus enhancin genes. Journal of General Virology 76(11): 2693–2705.CrossRefGoogle ScholarPubMed
Salama, HS, Foda, MS and Sharaby, A (1985) Potential of some chemicals to increase the effectiveness of Bacillus thuringiensis Berl. against Spodopter littorlis. Zeitschrift für Angewandte Entomologie 100(2): 425–433.CrossRefGoogle Scholar
Sambrook, J, Fritsch, EF and Maniatis, T (1989) Molecular Cloning: A Laboratory Manual, 2nd ed. New York: Cold Spring Harbor Laboratory Press, pp. 16–57.Google Scholar
Tang, QY and Feng, MG (1997) Practical Statistics and DPS Data Processing System. Beijing: China Agricultural Press, pp. 39–254 (in Chinese).Google Scholar
Wang, P and Granados, RR (1997) An intestinal mucin is the target for a baculovirus enhancin. Proceedings of the National Academy of Sciences USA 94(14): 6977–6982.CrossRefGoogle ScholarPubMed
Yamamoto, T and Tanada, Y (1980) Physicochemical properties and location of capsule components, in particular the synergistic factor, in the inclusion body of a granulosis virus of the armyworm, Psedaletia unipuncta. Virology 107(2): 434–440.CrossRefGoogle Scholar
Yuan, ZM, Meng, XL and Liu, SS (2000) Synergy of recombinant enhancin of Trichoplusia ni Granulovirus. Virologica Sinica 15 (Special Issue): 55–60 (in Chinese with English abstract).Google Scholar
Yuan, ZM, Meng, XL and Liu, SS (2001) Cloning and expression of 2.5 kb enhancin gene from Trichoplusia ni granulosis virus in Escherichia coli. Acta Entomologica Sinica 44(2): 155–160 (in Chinese with English abstract).Google Scholar