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Evaluation of cardiac electrophysiological properties in an experimental model of right ventricular hypertrophy and failure*

Published online by Cambridge University Press:  14 April 2015

Jacob G. Schultz Open the ORCID record for Jacob G. Schultz [Opens in a new window] ,
Stine Andersen ,
Asger Andersen ,
Jens Erik Nielsen-Kudsk  and
Jan M. Nielsen
Jacob G. Schultz*
Affiliation:
Department of Cardiology, Institute of Clinical Medicine, Aarhus University Hospital, Denmark
Stine Andersen
Affiliation:
Department of Cardiology, Institute of Clinical Medicine, Aarhus University Hospital, Denmark
Asger Andersen
Affiliation:
Department of Cardiology, Institute of Clinical Medicine, Aarhus University Hospital, Denmark
Jens Erik Nielsen-Kudsk
Affiliation:
Department of Cardiology, Institute of Clinical Medicine, Aarhus University Hospital, Denmark
Jan M. Nielsen
Affiliation:
Department of Cardiology, Institute of Clinical Medicine, Aarhus University Hospital, Denmark
*
Correspondence to: J. G. Schultz, Department of Cardiology–Research, Aarhus University Hospital, Brendstrupgaardsvej 100, 8200 Aarhus N, Denmark. Tel: +4529702879; Fax: +4578452260; E-mail: [email protected]
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Abstract

Background

Malignant arrhythmias are a major cause of sudden cardiac death in adults with congenital heart disease. We developed a model to serially investigate electrophysiological properties in an animal model of right ventricular hypertrophy and failure.

Method

We created models of compensated (cHF; n=11) and decompensated (dHF; n=11) right ventricular failure in Wistar rats by pulmonary trunk banding. Healthy controls underwent sham operation (Control; n=13). Surface electrocardiography was recorded from extremities, and inducibility of ventricular tachycardia was evaluated in vivo by programmed stimulation. Isolated right ventricular myocardium was analysed for mRNA expression of selected genes.

Results

Banding caused an increased mRNA expression of both connexin 43 and the voltage-gated sodium channel 1.5, as well as a prolongation of PQ, QRS and QTc intervals. Ventricular tachycardia was induced in the majority of banded animals compared with none in the healthy control group. No differences were found between the two degrees of failure in neither the electrophysiological parameters nor inducibility.

Conclusions

The electrophysiological properties of rat hearts subjected to pulmonary trunk banding were significantly changed with increased susceptibility to ventricular tachycardia, but no differences were found between compensated and decompensated right ventricular failure. Furthermore, we demonstrate that in vivo electrophysiological evaluation is a sensitive method to characterise the cardiac electric phenotype in an experimental rat model.

Keywords

Heart failureventricular tachycardiapulmonary trunk bandingadults with congenital heart diseaseright ventricle
Type
Original Articles
Information
Cardiology in the Young , Volume 26 , Issue 3 , March 2016 , pp. 451 - 458
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Copyright
© Cambridge University Press 2015 

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Footnotes

*

All authors take responsibility for all aspects of the reliability and freedom from bias of the data presented and their discussed interpretation.

†

Both authors contributed equally.

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