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Common signatures for gene expression in postnatal patients with patent arterial ducts and stented arteries

Published online by Cambridge University Press:  01 August 2009

Peter P. Mueller ,
Andreas Drynda ,
Diane Goltz ,
René Hoehn ,
Hansjörg Hauser  and
Matthias Peuster
Peter P. Mueller
Affiliation:
Helmholtz Centre for Infection Research, Braunschweig, Germany
Andreas Drynda
Affiliation:
Department of Pediatric Cardiology and Intensive Care, Children′s Hospital, University of Rostock, Rostock, Germany
Diane Goltz
Affiliation:
Clinic for Congenital Heart Defects, Herz- und Diabeteszentrum, Bad Oeynhausen, Germany
René Hoehn
Affiliation:
Department of Pediatric Cardiology and Intensive Care, Children′s Hospital, University of Rostock, Rostock, Germany
Hansjörg Hauser
Affiliation:
Helmholtz Centre for Infection Research, Braunschweig, Germany
Matthias Peuster*
Affiliation:
Department of Pediatric Cardiology and Intensive Care, Children′s Hospital, University of Rostock, Rostock, Germany
*
Correspondence to: Prof. Dr. med. Matthias Peuster, University of Rostock, Children′s Hospital, Department of Pediatric Cardiology and Intensive Care, Rembrandtstr. 16/17, D-18057 Rostock, Germany. Tel: +49(0)381-494-7201; Fax: +49(0)381-494-7202; E-mail: [email protected]
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Abstract

The detailed molecular processes associated with postnatal remodelling of blood vessels are presently not understood. To characterize the response of the patients undergoing stenting of the patent arterial duct, we harvested samples of vascular tissue during surgical repair. Histological analysis of explanted ducts confirmed the patency of the ducts immediately after birth. As expected, a previously unstented duct that was examined 7 months after birth had become closed and ligamentous. Whole genome expression profiling of these samples showed that a large fraction, over 10%, of the gene sequences examined were expressed differentially between the samples taken from patients with open as opposed to the ligamentous duct. Interestingly, in 2 patients in whom closure was prevented by insertion of stents, one showed an expression profile that was similar to that of the patient initially having an unstented open duct, whereas the other was more closely related to the profile of the patient with a duct that had become ligamentous. Moreover, in 2 specimens obtained from patients with stented pulmonary arteries, a large fraction of the genes that were differentially expressed were identical to the pattern seen in the samples from the patients with open ducts. The gene regulation appeared to be independent of the nature of the respective malformations, and the site of implantation of the stents. These findings suggest that a set of differentially expressed genes are indicative for a transcriptional programme in neonatal remodelling of the arterial duct, which may also take place in patients in whom ductal closure is prevented by stents, or in those with stented pulmonary arteries. The differentially expressed genes included a significant number of extracellular matrix synthetic genes, and could therefore be predictive for vascular remodelling and neointimal formation.

Keywords

Cardiovascular stentsbiomaterialsgene profilingcongenital heart disease
Type
Original Articles
Information
Cardiology in the Young , Volume 19 , Issue 4 , August 2009 , pp. 352 - 359
Copyright
Copyright © Cambridge University Press 2009

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References

1.Blalock, A, Taussig, HB. The surgical treatment of malformations of the heart in which there is pulmonary stenosis or pulmonary atresia. JAMA 1945; 128: 189–202.CrossRefGoogle Scholar
2.Levin, M, McCurnin, D, Seidner, SR, et al. Postnatal constriction, ATP depletion, and cell death in the mature and immature ductus arteriosus. Am J Physiol Regul Integr Comp Physiol 2006; 290: R359–R364.CrossRefGoogle ScholarPubMed
3.Hermes-DeSantis, ER, Clyman, RI. Patent ductus arteriosus: pathophysiology and management. J Perinatol 2006; 26 Suppl 1: S14–S18.CrossRefGoogle ScholarPubMed
4.Alwi, M, Choo, KK, Latiff, HA, Kandavello, G, Samion, H, Mulyadi, MD. Initial results and medium-term follow-up of stent implantation of patent ductus arteriosus in duct-dependent pulmonary circulation. J Am Coll Cardiol 2004; 44: 438–445.CrossRefGoogle ScholarPubMed
5.Galantowicz, M, Cheatham, JP. Lessons learned from the development of a new hybrid strategy for the management of hypoplastic left heart syndrome. Pediatric Cardiol 2005; 26: 190–199.CrossRefGoogle ScholarPubMed
6.Gewillig, M, Boshoff, DE, Dens, J, Mertens, L, Benson, LN. Stenting the neonatal arterial duct in duct-dependent pulmonary circulation: new techniques, better results. J Am Coll Cardiol 2004; 43: 107–112.CrossRefGoogle ScholarPubMed
7.Akintuerk, H, Michel-Behnke, I, Valeske, K, et al. Stenting of the arterial duct and banding of the pulmonary arteries : basis for combined Norwood stage I and II repair in hypoplastic left heart. Circulation 2002; 105: 1099–1103.CrossRefGoogle Scholar
8.Schneider, M, Zartner, P, Sidiropoulos, A, Konertz, W, Hausdorf, G. Stent implantation of the arterial duct in newborns with duct-dependent circulation. Eur Heart J 1998; 19: 1401–1409.CrossRefGoogle ScholarPubMed
9.Michel-Behnke, I, Akintuerk, H, Thul, J, Bauer, J, Hagel, KJ, Schranz, D. Stent implantation in the ductus arteriosus for pulmonary blood supply in congenital heart disease. Catheter Cardiovasc Interv 2004; 61: 242–252.CrossRefGoogle ScholarPubMed
10.Gibbs, JL, Uzun, O, Blackburn, ME, Wren, C, Hamilton, JR, Watterson, KG. Fate of the stented arterial duct. Circulation 1999; 99: 2621–2625.CrossRefGoogle ScholarPubMed
11.Adams, LD, Geary, RL, Li, J, Rossini, A, Schwartz, SM. Expression profiling identifies smooth muscle cell diversity within human intima and plaque fibrous cap: loss of RGS5 distinguishes the cap. Arterioscler Thromb Vasc Biol 2006; 26: 319–325.CrossRefGoogle ScholarPubMed
12.Hofmann, CS, Sullivan, CP, Jiang, HY, et al. B-Myb represses vascular smooth muscle cell collagen gene expression and inhibits neointima formation after arterial injury. Arterioscler Thromb Vasc Biol 2004; 24: 1608–1613.CrossRefGoogle ScholarPubMed
13.Geary, RL, Wong, JM, Rossini, A, Schwartz, SM, Adams, LD. Expression profiling identifies 147 genes contributing to a unique primate neointimal smooth muscle cell phenotype. Arterioscler Thromb Vasc Biol 2002; 22: 2010–2016.CrossRefGoogle ScholarPubMed
14.Zohlnhöfer, D, Klein, CA, Richter, T, et al. Gene expression profiling of human stent-induced neointima by cDNA array analysis of microscopic specimens retrieved by helix cutter atherectomy: Detection of FK506-binding protein 12 upregulation. Circulation 2001; 103: 1396–1402.CrossRefGoogle ScholarPubMed
15.Zohlnhofer, D, Richter, T, Neumann, F, et al. Transcriptome analysis reveals a role of interferon-gamma in human neointima formation. Molecular Cell 2001; 7: 1059–1069.CrossRefGoogle ScholarPubMed
16.May, T, Hauser, H, Wirth, D. Transcriptional control of SV40 T-antigen expression allows a complete reversion of immortalization. Nucleic Acids Res 2004; 32: 5529–5538.CrossRefGoogle ScholarPubMed
17.Mueller, PP, May, T, Perz, A, Hauser, H, Peuster, M. Control of smooth muscle cell proliferation by ferrous iron. Biomaterials 2006; 27: 2193–2200.CrossRefGoogle ScholarPubMed
18.Zhong, S, Storch, KF, Lipan, O, Kao, MC, Weitz, CJ, Wong, WH. GoSurfer: a graphical interactive tool for comparative analysis of large gene sets in gene ontologyTM space. Appl Bioinform 2004; 3: 261–264.CrossRefGoogle Scholar
19.Hosack, DA, JrDennis, G, Sherman, BT, Lane, HC, Lempicki, RA. Identifying biological themes within lists of genes with EASE. Genome Biology 2003; 4: R70.CrossRefGoogle ScholarPubMed
20.Eisen, MB, Spellman, PT, Brown, PO, Botstein, D. Cluster analysis and display of genome-wide expression patterns. Proc Natl Acad Sci USA 1998; 95: 14863–14868.CrossRefGoogle ScholarPubMed
21.Copois, V, Bibeau, F, Bascoul-Mollevi, C, et al. Impact of RNA degradation on gene expression profiles: assessment of different methods to reliably determine RNA quality. J Biotechnol 2007; 127: 549–559.CrossRefGoogle ScholarPubMed
22.Imbeaud, S, Graudens, E, Boulanger, V, et al. Towards standardization of RNA quality assessment using user-independent classifiers of microcapillary electrophoresis traces. Nucleic Acids Res 2005; 33: e56.CrossRefGoogle ScholarPubMed
23.Heidecker, B, Kasper, EK, Wittstein, IS, et al. Transcriptomic biomarkers for individual risk assessment in new-onset heart failure. Circulation 2008; 118: 238–246.CrossRefGoogle ScholarPubMed
24.Wang, J. Computational biology of genome expression and regulation--a review of microarray bioinformatics. J Environ Pathol Toxicol Oncol 2008; 27: 157–179.CrossRefGoogle ScholarPubMed
25.Ross, R, Klebanoff, SJ. The smooth muscle cell. I. In vivo synthesis of connective tissue proteins. J Cell Biol 1971; 50: 159–171.CrossRefGoogle ScholarPubMed
26.Stepp, MA, Kindy, MS, Franzblau, C, Sonenshein, GE. Complex regulation of collagen gene expression in cultured bovine aortic smooth muscle cells. J Biol Chem 1986; 261: 6542–6547.CrossRefGoogle ScholarPubMed
27.Liau, G, Chan, LM. Regulation of extracellular matrix RNA levels in cultured smooth muscle cells. Relationship to cellular quiescence. J Biol Chem 1989; 264: 10315–10320.CrossRefGoogle ScholarPubMed
28.Thyberg, J. Phenotypic modulation of smooth muscle cells during formation of neointimal thickenings following vascular injury. Histol Histopathol 1998; 13: 871–891.Google ScholarPubMed
29.Rocnik, EF, Chan, BM, Pickering, JG. Evidence for a role of collagen synthesis in arterial smooth muscle cell migration. J Clin Invest 1998; 101: 1889–1898.CrossRefGoogle ScholarPubMed
30.Yoshida, T, Owens, GK. Molecular determinants of vascular smooth muscle cell diversity. Circ Res 2005; 96: 280–291.CrossRefGoogle ScholarPubMed
31.Mulvihill, ER, Jaeger, J, Sengupta, R, et al. Atherosclerotic plaque smooth muscle cells have a distinct phenotype. Arterioscler Thromb Vasc Biol 2004; 24: 1283–1289.CrossRefGoogle ScholarPubMed
32.Hao, H, Gabbiani, G, Bochaton-Piallat, ML. Arterial smooth muscle cell heterogeneity: implications for atherosclerosis and restenosis development. Arterioscler Thromb Vasc Biol 2003; 23: 1510–1520.CrossRefGoogle ScholarPubMed