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Strategies for the Identification of Novel Brain Specific Genes Affected in Alzheimer Disease

Published online by Cambridge University Press:  18 September 2015

D.G. Walker ,
B.E. Boyes ,
P.L. McGeer  and
E.G. McGeer
D.G. Walker*
Affiliation:
Kinsmen Laboratory of Neurological Research, Department of Psychiatry, University of British Columbia, Vancouver
B.E. Boyes
Affiliation:
Kinsmen Laboratory of Neurological Research, Department of Psychiatry, University of British Columbia, Vancouver
P.L. McGeer
Affiliation:
Kinsmen Laboratory of Neurological Research, Department of Psychiatry, University of British Columbia, Vancouver
E.G. McGeer
Affiliation:
Kinsmen Laboratory of Neurological Research, Department of Psychiatry, University of British Columbia, Vancouver
*
Kinsmen Laboratory of Neurological Research, Department of Psychiatry, U.B.C., Vancouver, British Columbia, Canada V6T 1W5
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Abstract:

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The pathological changes that occur in Alzheimer disease (AD) brain lead to a large loss of various classes of neurons and the production of novel proteinaceous elements such as neuritic plaques and neurofibrillary tangles. For the neuronal loss to occur and these elements to arise, there must be a disturbance in the expression or regulation of genes that code for proteins required for normal cell maintenance, or perhaps even for the expression of genes unique to AD. We describe the construction of a cDNA library from the human substantia innominata and strategies for isolating genes that are expressed differentially between brain regions and which may be affected by AD. Some of the results obtained using these strategies and a preliminary description of a novel brain specific mRNA of 15.5kb, whose expression is increased in AD affected temporal cortex, are presented.

Type
Research Article
Information
Canadian Journal of Neurological Sciences , Volume 16 , Issue S4 , November 1989 , pp. 483 - 489
Copyright
Copyright © Canadian Neurological Sciences Federation 1989

References

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