Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-11-23T20:51:01.185Z Has data issue: false hasContentIssue false
  • English
  • Français

Interpretation of Single Band Patterns in CSF Protein Electrophoresis

Published online by Cambridge University Press:  18 September 2015

B.H. Bass ,
H. Armstrong ,
B. Weinshenker ,
G.C. Ebers ,
J.H. Noseworthy  and
G.P.A. Rice
B.H. Bass
Affiliation:
Department of Clinical Neurological Sciences, University Hospital, London
H. Armstrong
Affiliation:
Department of Clinical Neurological Sciences, University Hospital, London
B. Weinshenker
Affiliation:
Department of Clinical Neurological Sciences, University Hospital, London
G.C. Ebers
Affiliation:
Department of Clinical Neurological Sciences, University Hospital, London
J.H. Noseworthy
Affiliation:
Department of Clinical Neurological Sciences, University Hospital, London
G.P.A. Rice*
Affiliation:
Department of Clinical Neurological Sciences, University Hospital, London
*
Department of Clinical Neurological Sciences, University Hospital, P.O. Box 5339, Postal Station A, London, Ontario, Canada N6A 5A5
Rights & Permissions [Opens in a new window]

Abstract:

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

We have examined the diagnostic significance of finding one band in the immunoglobulin (IgG) region in spinal fluid protein electrophoresis. From January 1983 to January 1986, 855 consecutive CSF electrophoreses were performed on as many patients. A blinded observer identified a single band in the IgG region in 53 cases (6.2%). In only 14 patients (26%), were the clinical features ultimately felt to be due to clinically definite or possible multiple sclerosis (MS). The majority of patients with a single band had another neurological diagnosis (55%) or were neurologically normal (6%). Many of the neurological disorders in which a single band was found were not disorders in which an increased intrathecal synthesis of immunoglobulin or electrophoretic restriction would have been expected. A variety of conditions can produce a single band pattern. The significance of these patterns and the means by which they might be identified are described.

Type
Original Articles
Information
Canadian Journal of Neurological Sciences , Volume 15 , Issue 1 , February 1988 , pp. 20 - 22
Copyright
Copyright © Canadian Neurological Sciences Federation 1988

References

REFERENCES

1.Ebers, GC. Oligoclonal banding in multiple sclerosis. Ann NY Acad Sci 1984, 436: 206–212.CrossRefGoogle Scholar
2.Lowenthal, A, Van Sande, M and Karcher, D. The differential diagnosis of neurological diseases by fractionating electrophoreti-cally the CSF-globulins. J Neurochem 1960; 6: 51–60.CrossRefGoogle Scholar
3.Johnson, KP, Arrigo, SC, Nelson, BJ, et al. Agarose electrophoresis of cerebrospinal fluid in multiple sclerosis. Neurology 1977; 27: 273–277.CrossRefGoogle ScholarPubMed
4.Hershey, LA, and Trotter, JL. The use and abuse of the cerebrospinal fluid IgG profile in the adult: A practical evaluation. Ann Neurol 1980; 8: 426–434.CrossRefGoogle ScholarPubMed
5.Rose, AS, Ellison, G, Myers, L, et al. Criteria for the clinical diagnosis of multiple sclerosis. Neurology 1976; 26: 20–22.CrossRefGoogle ScholarPubMed
6.Voulters, L, Ebers, GC, Neurology 1984; 34 (Suppl): 227 (abstract).Google Scholar
7.Mattson, DH, Roos, RP, Arnason, BGW. Immunoperoxidase staining in cerebrospinal fluid IgG in isoelectric focusing gels. A sensitive new technique. J Neurosci Methods 1980; 3: 67–75.CrossRefGoogle ScholarPubMed
8.Graham, RC, Lundholm, U, Karnovsky, MJ. Cytochemical demonstration of peroxidase activity with 3 amino-9-ethylcarbazole. J Histochem Cytochem 1965; 13: 150–152.CrossRefGoogle ScholarPubMed
9.Link, H. Immunoglobulin G and low molecular weight proteins in human cerebrospinal fluid. Acta Neurol Scand 1967; 43 (Suppl.28): 1–136.Google ScholarPubMed
10.Hochwald, GM, and Thorbecke, GJ. Trace proteins in cerebrospinal fluid and other biological fluids. II Effect of storage and enzymes on the electrophoretic mobility of gamma trace and beta trace proteins in cerebrospinal fluid. Clin Chim Acta 1963; 678–684.CrossRefGoogle ScholarPubMed
11.Laterre, EC, and Heremans, JF. A note on proteins apparently “specific” for cerebrospinal fluid. Clin Chim Acta 1963; 8: 220.CrossRefGoogle Scholar