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Intermediate Filaments in the Human Pituitary Gland: An Immunohistochemical Study

Published online by Cambridge University Press:  18 September 2015

W.C. Halliday ,
S.L. Asa ,
K. Kovacs  and
B.W. Scheithauer
W.C. Halliday*
Affiliation:
Departments of Pathology, University of Manitoba
S.L. Asa
Affiliation:
St. Michael's Hospital, Toronto
K. Kovacs
Affiliation:
St. Michael's Hospital, Toronto
B.W. Scheithauer
Affiliation:
Mayo Clinic, Rochester
*
Department of Pathology (Neuropathology), Health Sciences Centre, MS-435-T, 820 Sherbrook Street, Winnipeg, Manitoba, Canada R3A 1R9
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Abstract:

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Normal pituitaries, pituitaries showing CHC and selected adenomas were assessed for their possible content of intermediate filaments (IF), using immunohistochemical techniques. Normal pituitaries were stained for each of the five known IF (Cytokeratins (CK), glial fibrillary acidic protein (GFAP), desmin, vimentin, and neurofilaments (NF)). Desmin could not be demonstrated, and NF staining was limited to processes in the posterior gland. In serial sections, CK was demonstrated in some corticotrophs, somatotrophs, and lactotrophs. Vimentin was limited to processes in the posterior gland. Folliculo-stellate cells (FSC), demonstrated by staining for SlOO protein, exhibited a small subpopulation staining for CK, and another subpopulation staining for GFAP. GFAP was also demonstrated in glial processes in the posterior gland. Cells showing Crooke's hyaline change stained intensely for CK. Null cell adenomas did not stain for CK. Corticotroph adenomas, somatotroph adenomas, with or without fibrous bodies, and lactotroph adenomas contain CK-immunoreactive cells. We conclude that CK is the major IF present in the anterior pituitary gland, within some normal corticotrophs, somatotrophs and lactotrophs, and also in adenomas derived from these cells.

Résumé:

RÉSUMÉ:

Des hypophyses normales, des hypophyses présentent des changements hyalins de Crooke (CHC) et des adénomes ont été évaluées quant à leur contenu en filaments intermédiaires (FI) au moyen de techniques immunohistochimiques. Les hypophyses normales ont été colorées pour chacun des cinq FI connus (cytokératines (CK), protéine gliale fibrillaire acide (GFAP), desmine, vimentine, et neurofilaments (NF)). La desmine n'a pu être mise en évidence et la coloration pour les NF était limitée aux terminaisons dans l'hypophyse postérieure. Dans des coupes sériées, les CK étaient présentes dans des cellules corticotrophes, somatotrophes et lactotrophes. La vimentine était limitée aux terminaisons dans l'hypophyse postérieure. Les cellules étoilées folliculaires (FSC) mises en évidence par la coloration pour la protéine S100, présentaient une petite sous-population qui prenait le colorant pour la GFAP. La GFAP a également été mise en évidence dans le prolongements gliaux dans l'hypophyse postérieure. Les cellules manifestant le CHC prenaient intensément la coloration pour les CK. Les adénomes à cellules nulles ne montraient pas de CK. Les adénomes corticotrophes, les adénomes somatotrophes, avec ou sans corps fibreux, et les adenomes lactotrophes contiennent des cellules CK-positives. Nous concluons que les CK sont le FI majeur présent dans l'hypophyse antérieure, dans des cellules corticotrophes, somatotrophes et lactotrophes normales et également dans des adénomes dérivés de ces cellules.

Type
Original Articles
Information
Canadian Journal of Neurological Sciences , Volume 17 , Issue 2 , May 1990 , pp. 131 - 136
Copyright
Copyright © Canadian Neurological Sciences Federation 1990

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