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During Repetitive Forebrain Ischemia, Post-ischemic Hypothermia Protects Neurons from Damage

Published online by Cambridge University Press:  18 September 2015

Ashfaq Shuaib ,
Sadiq Ijaz ,
Jay Kalra  and
William Code
Ashfaq Shuaib*
Affiliation:
Departments of Medicine (Division of Neurology), College of Medicine, University of Saskatchewan, Saskatoon
Sadiq Ijaz
Affiliation:
Anesthesia, Cerebrovasular Research Laboratory, College of Medicine, University of Saskatchewan, Saskatoon
Jay Kalra
Affiliation:
Pathology, College of Medicine, University of Saskatchewan, Saskatoon
William Code
Affiliation:
Saskatchewan Stroke Research Centre, College of Medicine, University of Saskatchewan, Saskatoon
*
Dept. of Medicine, Royal University Hospital, Saskatoon, Saskatchewan, Canada S7N 0X0
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Abstract:

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In rodents damage from repetitive transient cerebral ischemia is more severe than that seen with a single ischemic insult of similar duration. Mild hypothermia has been shown to be very effective in protecting the brain during single ischemic insults. We tested the protective effects of hypothermia in repetitive ischemic insults. We used the gerbil model of repetitive ischemia (three minutes ischemia repeated at one hourly intervals three times) and histological evaluation was done using the silver staining technique. Our study reveals that a decrease in body and scalp temperature by 1-2 degrees Celsius can significantly reduce neuronal damage in the cerebral cortex, CA1 region of the hippocampus and substantia nigra reticulata during repetitive ischemia. As the hypothermia was induced after the initial insult, we believe this offers an opportunity for intervention in the clinical settings.

Type
Articles
Information
Canadian Journal of Neurological Sciences , Volume 19 , Issue 4 , November 1992 , pp. 428 - 432
Copyright
Copyright © Canadian Neurological Sciences Federation 1992

References

REFERENCES

1.Araki, T, Kato, H, Kogure, K.Neuronal damage and calcium accumulation following repeated brief cerebral ischemia in the gerbil. Brain Res 1990; 528: 114–122.CrossRefGoogle ScholarPubMed
2.Nakano, S, Kato, H, Kogure, K.Neuronal damage in the rat hippocampus in a new model of repeated reversible transient cere-bral ischemia. Brain Res 1989; 490: 178–180.CrossRefGoogle Scholar
3.Shuaib, A, Ijaz, S, Kalra, J, Code, W.Substantia nigra reticulata damage is delayed in repetitive cerebral ischemia. Brain Res 1992; 574: 120–124.CrossRefGoogle Scholar
4.Young, RSK, Olenginski, TP, Yagel, SK, Towfighi, J.The effect of graded hypothermia on the hypoxic-ischemic brain damage: a neuropathology study in the neonatal rat. Stroke 1983; 14: 929–934.CrossRefGoogle Scholar
5.Herschkowitz, FSN, Stoller, EBM, Cross, TA, Aue, WP, Seelig, J.Cerebral metabolic studies in situ by 31p-nuclear magnetic resonance after hypothermia circulatory arrest. Pediatric Res 1986; 20: 867–871.Google Scholar
6.Shuaib, A, Hertz, L, Code, WE.Hypothermia protects astrocytes in cell culture during hypoxia. Ann Neurol 1991; 30: 319.Google Scholar
7.Busto, R, Dietrich, WD, Globus, MYT, Valdês, I, Scheinberg, P, et al. Small differences in intraischemic brain temperature critically determine the extent of ischemic neuronal injury. J Cereb Blood Flow Metab 1987; 7: 729–738.CrossRefGoogle ScholarPubMed
8.Chopp, M, Chen, H, Dereski, MO, Garcia, JH.Mild hypothermic intervention after graded ischemic stress in rats. Stroke 1991; 22: 37–43.CrossRefGoogle ScholarPubMed
9.Gallyas, F, Wolff, JR, Bottcher, H, Zaborszby, L.A reliable method to localize axonal damage after axotomy. Stain Tech 1980; 55: 291–297.CrossRefGoogle Scholar
10.Gallyas, F, Wolff, JR, Bottcher, H, Zaborszky, L.A reliable and sensitive method to localize terminal degeneration and lysosomes in the central nervous system. Stain Tech 1980; 55: 299–306.CrossRefGoogle ScholarPubMed
11.Paxinos, G, Watson, C.The Rat Brain in Stereotaxic Coordinates. Sydney, New York: Academic Press, 1982.Google Scholar
12.Loskota, WJ, Lomax, P, Verity, MA.A Stereotaxic Atlas of the Mongolian Gerbil Brain. Ann Arbor: Ann Arbor Publishers Inc., 1974.Google Scholar
13.Pulsinelli, WA, Brierly, JB, Plum, F.Temporal profile of neuronal damage in a model of transient forebrain ischemia. Ann Neurol 1982; 11: 491–498.CrossRefGoogle Scholar
14.Kato, H, Araki, T, Kogure, K.Postischemic spontaneous hyperthermia is not a major aggravating factor for neuronal damage following repeated brief cerebral ischemia in the gerbil. Neurosci Lett 1991; 126: 21–24.CrossRefGoogle ScholarPubMed
15.Kitagawa, K, Matsumoto, M, Tagaya, M, et al. Ischemic tolerance phenomenon found in the brain. Brain Res 1990; 528: 21–24.CrossRefGoogle ScholarPubMed
16.Kopf, GS, Mirvis, DM, Myers, RE.Central nervous tolerance to cardiac arrest during profound hypothermia. J Surg Res 1975; 18: 29–34.CrossRefGoogle ScholarPubMed
17.Busto, R, Globus, MYT, Dietrich, WD, Martinez, E, Valdês, I, et al. Effects of mild hypothermia on ischemia-induced release of neuro-transmitters and free fatty acids in rat brain. Stroke 1989; 20: 904–910.CrossRefGoogle Scholar
18.Buchan, A, Pulsinelli, WA.Hypothermia but not NMDA antagonist MK-801, attenuates neuronal damage in gerbils subjected to transient global ischemia. J Neurosci 1990; 10: 311–316.CrossRefGoogle Scholar
19.Marshall, SB, Swan, H.Temporary circulatory occlusion to the brain of the hypothermic dog. Arch Surg 1956; 72: 98–106.CrossRefGoogle Scholar
20.Leonov, Y, Sterz, F, Safar, P, et al. Mild cerebral hypothermia during and after cardiac arrest improves neurologic outcome in dogs. J Cereb Blood Flow Metab 1990; 10: 57–70.CrossRefGoogle ScholarPubMed
21.Busto, RBS, Dietrich, D, Globus, M Y-T, Valdês, I, Scheinberg, P, et al. Small differences in intraischemic temperatures critically determine the extent of ischemic neuronal injury. J Cereb Blood Flow Metab 1987; 7: 729–738.CrossRefGoogle ScholarPubMed
22.Hagedal, M, Welsh, FA, Keykhah, MM, Perez, E, Harp, JR.Protective effects of combinations of hypothermia and barbiturates in cerebral hypoxia in the rat. Anesthesiology 1978; 49: 165–169.CrossRefGoogle Scholar
23.Bailes, JE, Leavitt, ML, Teeple, E Jr, et al. Ultraprofound hypothermia with complete blood substitution in a canine model. J Neurosurg 1991; 74: 781–788.CrossRefGoogle Scholar
24.Clifton, GL, Taft, WC, Blair, RE, Choi, SC, DeLorenzo, RJ.Conditions for pharmacological evaluation in the gerbil model of forebrain ischemia. Stroke 1989; 20: 1545–1552.CrossRefGoogle ScholarPubMed
25.Vacanti, FX, Ames, III A.. Mild hypothermia and Mg protects against irreversible damage during CNS ischemia. Stroke 1984; 15: 695–698.CrossRefGoogle ScholarPubMed
26.Busto, RBS, Dietrich, D, Globus, M Y-T, Ginsberg, M.Postischemic moderate hypothermia inhibits CA1 hippocampal ischemic neuronal injury. Neurosci Lett 1989; 101: 299–304.CrossRefGoogle ScholarPubMed
27.Natale, JE, D’Alecy, LG.Protection from cerebral ischemia by brain cooling without reduced lactate accumulation in dogs. Stroke 1989; 20: 770–777.CrossRefGoogle ScholarPubMed
28.Lim, B, Globus, M Y-T, Dietrich, D, et al. Different morphological and neurochemical sequelae of global ischemia: comparison of single and multiple paradigms. J Cereb Blood Flow Metab 1991; 11 (Suppl 2): S854.Google Scholar
29.Busto, RBS, Globus, M Y-T, Dietrich, D, Martinez, E, Valdês, I, Ginsberg, M.Effects of mild hypothermia on ischemia induced release of neurotransmitters and free fatty acids in the rat brain. Stroke 1989; 20: 904–910.CrossRefGoogle ScholarPubMed
30.Dietrich, D, Busto, R, Valdês, I, Loor, Y.Effects of normothermic versus mild hyperthermic forebrain ischemia in rats. Stroke 1990; 21: 1318–1325.CrossRefGoogle ScholarPubMed
31.Crain, BJ, Westerkam, WD, Harrison, AH, Nadler, JV.Selective neuronal death after transient forebrain ischemia in the Mongolian gerbil: a silver impregnation study. Neuroscience 1988; 27: 387–402.Google Scholar