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VIRULENCE OF ENTOMOPATHOGENIC NEMATODE–BACTERIA COMPLEXES FOR LARVAE OF NOCTUIDS, A GEOMETRID, AND A PYRALID1

Published online by Cambridge University Press:  31 May 2012

O.N. Morris ,
V. Converse  and
J. Harding
O.N. Morris
Affiliation:
Agriculture Canada Research Station, Winnipeg, Manitoba, Canada R3T 2M9
V. Converse
Affiliation:
Agriculture Canada Research Station, Winnipeg, Manitoba, Canada R3T 2M9
J. Harding
Affiliation:
Agriculture Canada Research Station, Winnipeg, Manitoba, Canada R3T 2M9
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Abstract

Nine entomopathogenic nematode–bacteria complexes were screened for their virulence against larvae of six noctuids, a geometrid, and a pyralid. Ninety-six-hour LD50’s of steinernematids (and heterorhabditids) ranged from 1 to 3 (1–8) infective juveniles in Galleria mellonella (L.), 1–71 (2) in Peridroma soucia (Hbn.), 1–10 (1–3) in Mamestra configurata (Wlk.), 1–28 (3–7) in Euxoa ochrogaster (Gn.), 19 in Lacanobia radix (Wlk.), 22–60 (4) in Pseudaletia unipuncta (Haw.), 2–95 (111) in Agrotis ipsilon (Hufn.), and 3–28 in Paleacrita vernata (Peck). The nematode, Steinernema feltiae LIC, a cold-hardy strain isolated in Newfoundland, was highly virulent for G. mellonella and M. configurata, but not for P. soucia and E. ochrogaster. The number of nematodes invading larvae and the number produced were greater in G. mellonella than in other insects tested. Nematode–bacteria complexes that showed potential for controlling cutworms included S. feltiae, S. glaseri, S. bibionis, Heterorhabditis bacteriophora, and H. heliothidis.

Résumé

Neuf complexes nématodes–bactéries entomopathogènes ont été examinés pour leur virulence envers les larves de six noctuelles, un géomètre et une pyrale. Des LD50 pour 96 h d’exposition aux steinernematidae (et aux heterorhabdidae) ont varié entre 1 et 3 (1 et 8) juvéniles infectueux chez Galleria mellonella (L.), 1 et 71 (2) chez Peridroma saucia (Hbn.), 1 et 10 (1 et 3) chez Mamestra configurata (Wlk.), 1 et 28 (3 et 7) chez Euxoa ochrogaster (Gn.), 19 chez Lacanobia radix (Wlk.), 22 et 60 (4) chez Pseudaletia unipuncta (Haw.), 2 et 95 (111) chez Agrotis ipsilon (Hufn.) et 3 et 28 chez Paleacrita vernata (Peck). Le nematode, Steinernema feltiae LIC, une souche tolérante au froid isolée à Terre-Neuve, a été hautement virulente pour G. mellonella et M. configurata, mais pas pour P. saucia et E. ochrogaster. Le nombre de nématodes infestant les larves et le nombre produits étaient plus élevés chez G. mellonella que chez les autres espèces testées. Les complexes nématodes–bactéries qui ont présenté un potentiel pour le contrôle des ver-gris sont S. feltiae, S. glaseri, S. bibionis, Heterorhabditis bacteriophora et H. heliothidis.

Type
Articles
Information
The Canadian Entomologist , Volume 122 , Issue 2 , April 1990 , pp. 309 - 319
Copyright
Copyright © Entomological Society of Canada 1990

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