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SEX PHEROMONE OF TRYPODENDRON LINEATUM (COLEOPTERA: SCOLYTIDAE): PRODUCTION, BIO-ASSAY, AND PARTIAL ISOLATION1

Published online by Cambridge University Press:  31 May 2012

John H. Borden ,
R. G. Brownlee  and
R. M. Silverstein
John H. Borden
Affiliation:
Department of Biological Sciences, Simon Fraser University, Burnaby, British Columbia
R. G. Brownlee
Affiliation:
Stanford Research Institute, Menlo Park, California
R. M. Silverstein
Affiliation:
Stanford Research Institute, Menlo Park, California
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Abstract

Overwintering adult T. lineatum were collected in late April, and the females allowed to attack Douglas-fir logs in the laboratory. Frass was collected and preserved at −40°C prior to extraction in benzene and distillation. Distillate was chromatographed in a silica-gel column. An olfactometer was developed in which a positive bio-assay involved arrestment and turning reactions in a runway over a perforated area, underneath which the test substance was placed. Responses occurred to female frass and to sawdust from host logs but frass was far more potent. Peak response occurred to frass produced within 4 to 8 hours after attack. After 2 weeks, only very small amounts of weakly attractive frass were produced. Benzene extract and distillate of female frass evoked trace responses at very low concentrations, hut at 3.2 × 10−2 and 7.4 ×10−3 g-equiv of frass, for extract and distillate respectively, responses over 70% were common. Two fractions of ether eluate from the silica-gel column contained a highly attractive component which evoked responses in about 70% of the test males.

Type
Articles
Information
The Canadian Entomologist , Volume 100 , Issue 6 , June 1968 , pp. 629 - 636
Copyright
Copyright © Entomological Society of Canada 1968

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References

Borden, J. H. 1967. Factors influencing the response of Ips confusus (Coleoptera: Scolytidae) to male attractant. Can. Ent. 99: 1164–1193.Google Scholar
Borden, J. H., Silverstein, R. M., and Brownlee, R. G.. 1968. Sex pheromone of Dendroctonus pseudotsugae (Coleoptera: Scolytidae): Production, bio-assay, and partial isolation. Can. Ent. This issue.Google Scholar
Castek, K. L., Barbour, J. F., and Rudinsky, J. A.. 1967. Isolation and purification of the attractant of the striped ambrosia beetle. J. econ. Ent. 60: 658–660.CrossRefGoogle Scholar
Chapman, J. A. 1960. The distribution of overwintering Trypodendron (Coleoptera, Scolytidae) around a single tree in relation to forest litter variability. Proc. ent. Soc. Br. Columb. 57: 3–6.Google Scholar
Chapman, J. A. 1966. The effect of attack by the ambrosia beetle Trypodendron lineatum (Oliver) on log attractiveness. Can. Ent. 98: 50–59.CrossRefGoogle Scholar
Dyer, E. D. A., and Chapman, J. A.. 1965. Flight and attack of the ambrosia beetle Trypodendron lineatum (Oliver) in relation to felling date of logs. Can. Ent. 97: 42–57.CrossRefGoogle Scholar
Francia, F. C., and Graham, K.. 1967. Aspects of orientation behavior in the ambrosia beetle Trypodendron lineatum (Oliver). Can. J. Zool. 45: 985–1002.Google Scholar
Graham, K. 1959. Release by flight exercise of a chemotropic response from photopositive domination in a scolytid beetle. Nature (Lond.) 184: 283–284.Google Scholar
Graham, K. 1960. Photic behavior in the ecology of the ambrosia beetle Trypodendron lineatum. Proc. 11th int. Congr. Ent. (Vienna). Vol. 2, p. 226.Google Scholar
Jantz, O. K., and Rudinsky, J. A.. 1965. Laboratory and field methods for assaying olfactory responses of the Douglas-fir beetle, Dendroctonus pseudotsugae Hopltins. Can. Ent. 97: 934–941.Google Scholar
Kinghorn, J. M., and Chapman, J. A.. 1959. The overwintering of the ambrosia beetle Trypodendron lineatum (Oliver). Forest Sci. 5: 81–92.Google Scholar
McBride, C. F. 1950. The effect of ambrosia beetle damage upon lumber value. Br. Columbia Lumberm. 34: 46–48, 122–128.Google Scholar
McBride, C. F., and Kinghorn, J. M.. 1960. Lumber degrade caused by ambrosia beetles. Br. Columbia Lumberm. 44: 40–52.Google Scholar
Rudinsky, J. A., and Daterman, G. E.. 1964 a. Field studies on flight patterns and olfactory responses of ambrosia beetles in Douglas-fir forests of western Oregon. Can. Ent. 96: 1339–1352.Google Scholar
Rudinsky, J. A., and Daterman, G. E.. 1964 b. Response of the ambrosia beetle Trypodendron lineatum (Oliver) to female-produced pheromone. Z. angew. Ent. 54: 300–303.CrossRefGoogle Scholar
Wood, D. L. et al. , 1966. Sex pheromones of bark beetles – I. Mass production, bio-assay, source, and isolation of the sex pheromone of Ips confusus (LeC.). J. Insect Physiol. 12: 523–536.CrossRefGoogle ScholarPubMed
Wood, D. L. et al. , 1968. Response of Ips confusus (LeConte) to synthetic sex pheromones in nature. Science 159: 1373–1374.CrossRefGoogle Scholar