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QUANTITATIVE STUDIES OF HYDROLYTIC ENZYME ACTIVITY IN THE SALIVARY GLAND OF CHIRONOMUS TENTANS (DIPTERA: CHIRONOMIDAE) DURING METAMORPHOSIS1

Published online by Cambridge University Press:  31 May 2012

Hans Laufer  and
Ki Ssu Schin
Hans Laufer
Affiliation:
Biological Sciences Group, University of Connecticut, Storrs and Marine Biological Laboratory, Woods Hole, Massachusetts
Ki Ssu Schin
Affiliation:
Biological Sciences Group, University of Connecticut, Storrs and Marine Biological Laboratory, Woods Hole, Massachusetts
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Abstract

Changes in the hydrolytic enzymes, ribonuclease and acid phosphatase were investigated in the salivary gland of Chironomus tentans since these lysosomal enzymes may participate in hormonally stimulated tissue breakdown. Quantitative assays revealed 9- and 12-fold increases in the specific activity of these enzymes during pupation while the protein content of the gland was decreasing. These increases cannot be accounted for by decreased protein but may represent an activation, accumulation, or synthesis which seems to be important in gland breakdown at metamorphosis.

Type
Articles
Information
The Canadian Entomologist , Volume 103 , Issue 3 , March 1971 , pp. 454 - 457
Copyright
Copyright © Entomological Society of Canada 1971

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