Published online by Cambridge University Press: 31 May 2012
In adult virgin females of the sugar beet wireworm, Limonius californicus (Mannerheim), the sex attractant (recently identified as valeric acid) is found primarily in the fifth and sixth abdominal segments. Ether extracts of virgin females and of females that had been mated for not more than 4 hours prior to death contained the highest concentration of the attractant. The concentration of attractant in mated females decreased as the post-copulatory interval increased. In the laboratory maximum sexual response was elicited from males exposed to as little as 0.008 female equivalent of attractant. Numerous thin-walled sensoria are present on the eight distal segments of the male antennae and apparently function as chemoreceptors. Response of males to the attractant was not affected by bilateral amputation of the five distal segments, decreased progressively with the amputation of segments 6 and 7, and was eliminated upon removal of segment 8. Males of L. californicus responded only to extracts of females of that species whereas males of Limonius canus (LeConte) responded to extracts of females of both species.