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PRODUCTION OF OPHIOSTOMA-FREE ADULTS OF IPS SEXDENTATUS BOERN. (COLEOPTERA: SCOLYTIDAE) AND COMPARISON WITH NATURALLY CONTAMINATED ADULTS

Published online by Cambridge University Press:  31 May 2012

Bénédicte Colineau  and
François Lieutier
Bénédicte Colineau
Affiliation:
INRA, Station de Zoologie Forestière, Ardon, F-45160 Olivet, France
François Lieutier*
Affiliation:
INRA, Station de Zoologie Forestière, Ardon, F-45160 Olivet, France
*
1Author to whom correspondence and reprint requests should be addressed.
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Abstract

This study determined the importance of Ophiostomatales fungi in the biology of individual adult Ips sexdentatus Boern. in Scots pine. We investigated factors possibly involved in success of rearing without Ophiostomatales, such as the sterilization technique, the presence of antibiotics, and the absence of yeasts. After sterilization of pupae, 866 adult beetles were reared in the media; the proportion of Ophiostoma-free mature adults was 99.2% and total mortality was about 13%. The most preferred rearing method used sterilized pupae which moulted on moistened filter paper and were transferred individually to separate vials where maturation took place on a sterilized phloem medium.The weight and size of Ophiostoma-free mature adults from semi-artificial media and, after establishment in logs, their fecundity and length of their egg galleries, were compared with those of naturally contaminated mature beetles obtained from logs. With the exception of a higher weight observed for adults matured in semi-artificial media, no essential difference was observed between the two groups. Ophiostomatales do not appear to play an essential role in the maturation and fecundity of I. sexdentatus adults.

Résumé

Le but de cette étude est de déterminer si les champignons Ophiostomatales jouent, au niveau des individus, un rôle important dans la biologie des adultes d’Ips sexdentatus sur le Pin sylvestre. Le rôle de divers facteurs dans le succès de l’élevage en absence d’Ophiostomatales a été testé, tels la technique de stérilisation, la présence d’antibiotiques et la présence de levures. La méthode d’élevage la mieux adaptée a été de placer les nymphes stérilisées sur papier filtre humide pour la mue imaginale puis, immédiatement après celle-ci, de transférer individuellement les jeunes adultes dans des pilluliers contenant un milieu axénique à base de phloème, pour leur maturation. Après stérilisation des nymphes, 866 adultes ont été élevés dans les milieux semi-artificiels. La proportion d’adultes mûrs non contaminés par Ophiostoma spp. a atteint 99.2% et la mortalité totale a été de 13%.Le poids et la taille des adultes murs indemnes d’Ophiostoma, ainsi que leur fécondité et la longueur de leur galerie de ponte après installation sur rondins, ont été comparés à ceux d’adultes contaminés obtenus à partir de rondins. A l’exception d’un poids plus élevé pour les adultes élevés sur milieu semi-artificiel, aucune différence n’a été observée entre les deux groupes. Les Ophiostomatales ne semblent donc pas jouer un rôle essentiel dans la maturation et la fécondité des adultes d’Ips sexdentatus.

Type
Articles
Information
The Canadian Entomologist , Volume 126 , Issue 1 , February 1994 , pp. 103 - 110
Copyright
Copyright © Entomological Society of Canada 1994

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