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INHIBITION OF CHOLINESTERASE IN THE CATTLE GRUB HYPODERMA BOVIS (DIPTERA: OESTRIDAE) AFTER TREATMENT OF THE HOST CATTLE WITH SYSTEMIC INSECTICIDES

Published online by Cambridge University Press:  31 May 2012

C. C. Steward
C. C. Steward
Affiliation:
Research Station, Canada Department of Agriculture, Lethbridge, Alberta
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Abstract

Cattle grubs, first-instar larvae of Hypoderma bovis (L.), were removed from nine slaughtered heifers. Two of these heifers had previously been treated with coumaphos, three with Ruelene, and two with trichlorfon. The remaining two heifers were not treated with insecticides and served as sources of control grubs. The grubs, after removal from the hosts and fixation in formalin, were incubated with one of the two cholinesterase substrates, acetylthiocholine iodide or 5-bromoindoxyl acetate. Cholinesterase was completely inhibited in the central nervous systems of the grubs whose hosts were treated with Ruelene. This inhibition was also virtually complete in grubs from heifers treated with trichlorfon and was usually complete in those from the heifers treated with coumaphos. This is presented as histochemical evidence that the three systemic insecticides inhibit the cholinergic enzymes in the grubs, thus causing them to die while still in the body of the host.

Type
Articles
Information
The Canadian Entomologist , Volume 101 , Issue 5 , May 1969 , pp. 463 - 466
Copyright
Copyright © Entomological Society of Canada 1969

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References

Beesley, W. N. 1967. Observations on the biology of the ox warble-fly (Hypoderma: Diptera, Oestridae). 1. In vitro culture of the first-instar larva. Ann. trop. Med. Parasit. 61: 176–181.CrossRefGoogle Scholar
Diegenbach, P. C. 1965. Use of inhibitors in cholinesterase histochemistry. Nature, Lond. 207: 308.CrossRefGoogle ScholarPubMed
Fraser, A. C. 1967. Pesticides. In Elliott, H. W. et al. (ed.), A. Rev. Pharmacol 7: 319–342.CrossRefGoogle Scholar
Pearse, A. G. E. 1960. Histochemistry theoretical and applied. 2nd ed. Churchill, London.Google Scholar
Steward, C. C. 1967. Distribution of cholinesterase in the larvae of Hypoderma bovis and Hypoderma lineatum (Diptera: Oestridae) and its inhibition by systemic insecticides. Can. Ent. 99: 340–350.CrossRefGoogle Scholar