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Field growth traits and insect-host plant interactions of two transgenic canola (Brassicaceae) lines with elevated trichome numbers

Published online by Cambridge University Press:  04 May 2016

U. Alahakoon
Affiliation:
Saskatoon Research Centre, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, Saskatchewan, S7N 0X2, Canada Department of Biology, University of Saskatchewan, 112 Science Place, Saskatoon, Saskatchewan, S7N 5E2, Canada
J. Adamson
Affiliation:
Department of Plant Science, University of Saskatchewan, 51 Campus Drive, Saskatoon, Saskatchewan, S7N 5A8, Canada
L. Grenkow
Affiliation:
Saskatoon Research Centre, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, Saskatchewan, S7N 0X2, Canada
J. Soroka*
Affiliation:
Saskatoon Research Centre, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, Saskatchewan, S7N 0X2, Canada
P. Bonham-Smith
Affiliation:
Department of Biology, University of Saskatchewan, 112 Science Place, Saskatoon, Saskatchewan, S7N 5E2, Canada
M. Gruber
Affiliation:
Saskatoon Research Centre, Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, Saskatchewan, S7N 0X2, Canada
*
3Corresponding author (e-mail: [email protected]).

Abstract

Plant growth and insect resistance characteristics were determined for two Brassica napus Linnaeus (Brassicaceae) lines, AtGL3+ and K-5-8, developed for enhanced trichome densities relative to their parental cultivar Westar. In the field, both transgenic lines had glabrous cotyledons that curled upwards at emergence but flattened with time, and young leaves with elevated trichome density. Flea beetle (Phyllotreta cruciferae (Goeze) and Phyllotreta striolata (Fabricius); Coleoptera: Chrysomelidae) feeding was reduced on true leaves of both lines by 30–50% compared with insecticide-free Westar. Flea beetle feeding levels on cotyledons of the two hairy-leaved lines were lower than on unprotected Westar and similar to those seen on insecticide-treated Westar. Antixenosis and antibiosis resistance was observed when diamondback moths (Plutella xylostella (Linnaeus); Lepidoptera: Plutellidae) interacted with the hairy AtGL3+ and K-5-8 lines in the laboratory. Although the numbers of eggs laid by female diamondback moths on the transformed lines were similar to or higher than on Westar, in feeding bioassays larvae moved off AtGL3+ plants and larval feeding injury decreased on the transformed lines compared with Westar leaves. No agronomic or seed yield penalties were found for plants of K-5-8. These data highlight the utility of manipulating trichome regulatory genes to increase plant resistance against brassicaceous insect pests.

Type
Insect Management
Copyright
© Her Majesty the Queen by Right of Canada 2016 

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Footnotes

Subject Editor: Kevin Floate

References

Agren, J. and Schemske, D.W. 1993. The cost of defense against herbivores: an experimental study of trichome production in Brassica rapa . The American Naturalist, 141: 338350.Google Scholar
Agren, J. and Schemske, D.W. 1994. Evolution of trichome number in a naturalized population of Brassica rapa . The American Naturalist, 143: 113.Google Scholar
Alahakoon, U.I. 2013. Effect of Transparent Testa GLABRA1 on trichome development, growth, and insect resistance in a Brassica napus AtGLABRA3 background. PhD thesis, University of Saskatchewan, Saskatoon, Saskatchewan, Canada. [online]. Available from http://ecommons.usask.ca/handle/10388/381/browse?value=Alahakoon%2C+Ushan&type=author [accessed 23 February 2016].Google Scholar
Alahakoon, U.I., Taheri, A., Nayidu, N.K., Epp, D., Yu, M., Parkin, I., et al. 2016. Hairy canola (Brasssica napus) re-visited: down-regulating TTG1 in an AtGL3-enhanced hairy leaf background improves growth, leaf trichome coverage, and metabolite gene expression diversity. BMC Plant Biology, 16: 12. doi:10.1186/s12870-015-0680-5.CrossRefGoogle Scholar
Anonymous. 2015. Cabbage stem flea beetle [online]. Home Grown Cereals Authority/Agriculture and Horticulture Development Board Information Sheet 24. Available from https://www.nfuonline.com/assets/44446 [accessed 10 December 2015].Google Scholar
Canola Council of Canada. 2014. Flea beetles. Canola encyclopedia. Canola Council of Canada [online]. Available from http://www.canolacouncil.org/canola-encyclopedia/insects/flea-beetles [accessed 10 December 2015].Google Scholar
Canola Council of Canada. 2015. Seeding rate. Canola encyclopedia [online]. Canola Council of Canada. Available from http://www.canolacouncil.org/canola-encyclopedia/crop-establishment/seeding-rate/ [accessed 10 December 2015].Google Scholar
Crop Protection Compendium. 2012. Phyllotreta striolata. CABI [online]. Available from http://www.cabi.org/cpc/datasheet/40784 [accessed 8 December 2015].Google Scholar
Crop Protection Compendium. 2015. Phyllotreta cruciferae. CABI [online]. Available from http://www.cabi.org/cpc/datasheet/40780 [accessed 8 December 2015].Google Scholar
Dosdall, L.M. 1994. Evidence for successful overwintering of diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), in Alberta. The Canadian Entomologist, 126: 183185.Google Scholar
Fakheran, S., Paul-Victor, C., Heichinger, C., Schmid, B., Grossniklaus, U., and Turnbull, L.A. 2010. Adaptation and extinction in experimentally fragmented landscapes. Proceedings of the National Academy of Sciences of the United States of America, 107: 1912019125.Google Scholar
Furlong, M.J., Wright, D.J., and Dosdall, L.M. 2013. Diamondback moth ecology and management: problems, progress, and prospects. Annual Review of Entomology, 58: 517541.Google Scholar
Gavloski, J.E. and Lamb, R.J. 2000a. Compensation for herbivory in cruciferous plants: specific responses to three defoliating insects. Economic Entomology, 29: 12731282.Google Scholar
Gavloski, J.E. and Lamb, R.J. 2000b. Compensation by cruciferous plants is specific to the type of simulated herbivory. Economic Entomology, 29: 12581267.Google Scholar
Gruber, M.Y., Wang, S., Ethier, S., Holowachuk, J., Bonham-Smith, P.C., Soroka, J., et al. 2006. ‘Hairy canola’- Arabidopsis GL3 induces a dense covering of trichomes on Brassica napus seedlings. Plant Molecular Biology, 60: 679698.Google Scholar
Hallett, R.H., Ray, H., Soroka, J.J., and Gruber, M.Y. 2005. Bioassay for assessing resistance of Arabidopsis thaliana L. (Heynh.) to the adult crucifer flea beetle, Phyllotreta cruciferae (Goeze) (Coleoptera: Chrysomelidae). Canadian Journal of Plant Science, 85: 225235.Google Scholar
Handley, R., Ekbom, B., and Agren, J. 2005. Variation in trichome density and resistance against a specialist insect herbivore in natural populations of Arabidopsis thaliana . Ecological Entomology, 30: 284292.CrossRefGoogle Scholar
Heimbach, U. and Müller, A. 2012. Incidence of pyrethroid-resistant oilseed rape pests in Germany. Pest Management Science, 69: 209216.Google Scholar
Henderson, A.M., Hallett, R.H., and Soroka, J.J. 2004. Pre-feeding behavior of the crucifer flea beetle, Phyllotreta cruciferae, on host and non-host crucifers. Journal of Insect Behavior, 17: 1739.Google Scholar
Johnson, H.B. 1975. Plant pubescence: an ecological perspective. Botanical Review, 41: 233258.Google Scholar
Klassen, A.J., Downey, R.K., and Capcara, J.J. 1987. Westar summer rape. Canadian Journal of Plant Science, 67: 491493.CrossRefGoogle Scholar
Knodel, J.J. and Olson, D.L. 2002. Crucifer flea beetle biology and integrated pest management in canola. North Dakota State University Extension Circular, E–1234: 14.Google Scholar
Lamb, R.J. 1984. Effects of flea beetles, Phyllotreta spp. (Coleoptera: Chrysomelidae), on the survival, growth, seed yield and quality of canola, rape and yellow mustard. The Canadian Entomologist, 116: 269280.Google Scholar
Lamb, R.J. and Turnock, W.J. 1982. Economics of insecticidal control of flea beetles (Coleoptera: Chrysomelidae) attacking rape in Canada. The Canadian Entomologist, 114: 827840.Google Scholar
Levin, D. 1973. The role of trichomes in plant defense. The Quarterly Review of Biology, 48: 315.Google Scholar
Madder, D.J. and Stemeroff, M. 1988. The economics of insect control on wheat, corn, and canola in Canada, 1980–1985. Bulletin of the Entomological Society of Canada, 20 (Supplement): 122.Google Scholar
Palaniswamy, P. and Bodnaryk, R.P. 1994. A wild Brassica from Sicily provides trichome-based resistance against flea beetles, Phyllotreta cruciferae (Goeze) (Coleoptera: Chrysomelidae). The Canadian Entomologist, 126: 11191130.Google Scholar
Palaniswamy, P. and Lamb, R.J. 1992. Host preferences of the flea beetles Phyllotreta cruciferae and P. striolata (Coleoptera: Chrysomelidae) for crucifer seedlings. Journal of Economic Entomology, 85: 743752.Google Scholar
Paul-Victor, C., Züst, T., Rees, M., Kliebenstein, D.J., and Turnbull, L.A. 2010. A new method for measuring relative growth rate can uncover the costs of defensive compounds in Arabidopsis thaliana . New Phytologist, 187: 11021111.Google Scholar
Plett, J.M., Wilkins, O., Campbell, M.M., Ralph, S.G., and Regan, S. 2010. Endogenous over-expression of Populus MYB186 increases trichome density, improves insect pest resistance, and impacts plant growth. The Plant Journal, 64: 419432.Google Scholar
Sarfraz, R.M., Dosdall, L.M., Keddie, A.W., and Myers, J.H. 2011. Larval survival, host plant preferences and developmental responses of diamondback moth Plutella xylostella (Lepidoptera: Plutellidae) on wild brassicaceous species. Entomological Science, 14: 2030.Google Scholar
SAS Institute. 2008. SAS user’s guide: version 9.2, SAS Institute, Cary, North Carolina, United States of America.Google Scholar
Sletvold, N., Huttmen, P., Handley, R., Kärkkäinen, K., and Agren, J. 2010. Cost of trichome production and resistance to a specialist insect herbivore in Arabidopsis lyrata . Evolutionary Ecology, 10: 13071319.Google Scholar
Soroka, J.J., Grenkow, L.F., and Irvine, R.B. 2008. Impact of decreasing ratios of insecticide-treated seed on flea beetle (Coleoptera: Chrysomelidae; Phyllotreta spp.) feeding levels and canola seed yields. Journal of Economic Entomology, 101: 18111820.Google Scholar
Soroka, J.J., Holowachuk, J.M., Gruber, M.Y., and Grenkow, L.F. 2011. Feeding by flea beetles (Coleoptera: Chrysomelidae; Phyllotreta spp.) is decreased on canola (Brassica napus) seedlings with increased trichome density. Journal of Economic Entomology, 104: 125136.Google Scholar
Talekar, N.S., Liu, S.-H., Chen, C.-L., and Yiin, Y.-F. 1994. Characteristics of oviposition of diamondback moth (Lepidoptera: Yponomeutidae) on cabbage. Zoological Studies, 33: 7277.Google Scholar
Talekar, N.S. and Shelton, A.M. 1993. Biology, ecology, and management of the diamondback moth. Annual Review of Entomology, 38: 275301.CrossRefGoogle Scholar
Tansey, J.A., Dosdall, L.M., Keddie, B.A., and Sarfraz, R.M. 2008. Differences in Phyllotreta cruciferae and Phyllotreta striolata (Coleoptera: Chrysomelidae) responses to neonicotinoid seed treatments. Journal of Economic Entomology, 101: 18111820.Google Scholar
Ulmer, B., Gillott, C., and Erlandson, M. 2001. Feeding preferences, growth, and development of Mamestra configurata (Lepidoptera: Noctuidae) on Brassicaceae. The Canadian Entomologist, 133: 509519.Google Scholar
Westdal, P.H. and Romanow, W. 1972. Observations on the biology of the flea beetle Phyllotreta cruciferae (Coleoptera: Chrysomelidae). Manitoba Entomologist, 6: 3445.Google Scholar
Woodman, R.L. and Fernandes, G.W. 1991. Differential mechanical defense: herbivory, evapotranspiration, and leaf-hairs. Oikos, 60: 1119.Google Scholar
Zalucki, M.P., Shabbir, A., Silva, R., Adamson, R., Shu-Sheng, L., and Furlong, M.K. 2012. Estimating the economic costs of one of the world’s major insect pests, Plutella xylostella (Lepidoptera: Plutellidae): just how long is a piece of string? Journal of Economic Entomology, 105: 11151129.Google Scholar