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DNA FINGERPRINTING OF SINGLE APHID EMBRYOS BY RANDOM AMPLIFIED POLYMORPHIC DNA POLYMERASE CHAIN REACTION (RAPD–PCR)

Published online by Cambridge University Press:  31 May 2012

C.K. Chan ,
D.J. Petersen  and
T.C. Vrain
C.K. Chan
Affiliation:
Department of Biological Sciences, Simon Fraser University, Burnaby, British Columbia, Canada V5A 1S6
D.J. Petersen
Affiliation:
Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, Summerland, British Columbia, Canada VOH 1ZO
T.C. Vrain
Affiliation:
Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, Summerland, British Columbia, Canada VOH 1ZO
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Extract

Extraction of DNA from whole aphids, in combination with random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) (Williams et al. 1990) markers can detect interspecific and intraspecific genetic variation (Black et al. 1992; Cenis et al. 1993). However, these techniques entail destructive sampling of fresh or preserved specimens. To allow experimental replication from a single sample while preserving the same aphid for morphometrical or karyotyping analyses, we describe a technique for RAPD-PCR using DNA from single aphid embryos. We evaluated the usefulness and reliability of single-embryo analysis, using four species of our laboratory colonies, namely Acyrthosiphon pisum (Harris), Aphis fabae Scopoli, Aphis frangulae group, and Aphis gossypii Glover.

Type
Articles
Information
The Canadian Entomologist , Volume 131 , Issue 2 , April 1999 , pp. 229 - 230
Copyright
Copyright © Entomological Society of Canada 1999

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References

Black, W.C. IV, DuTeau, N.M., Puterka, G.J., Nechols, J.R., Pettorini, J.M. 1992, Use of the random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) to detect DNA polymorphisms in aphids. Bulletin of Entomological Research 82: 151–59.CrossRefGoogle Scholar
Cenis, J.L., Perez, P., Fereres, A. 1993. Identification of aphid (Homoptera: Aphididae) species and clones by random amplified polymorphic DNA. Annals of the Entomological Society of America 86: 545–50.Google Scholar
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