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THE DIGESTIVE PROCESSES OF HAEMATOPHAGOUS INSECTS: IV. SECRETION OF TRYPSIN BY AEDES AEGYPTI (DIPTERA: CULICIDAE)

Published online by Cambridge University Press:  31 May 2012

R. H. Gooding
R. H. Gooding
Affiliation:
Department of Entomology, University of Alberta, Edmonton
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Abstract

There is a correlation between the amount of blood ingested by Aedes aegypti (L.) and the trypsin activity (but not the chymotrypsin activity) in the midgut 16 and 24 hr after feeding. Puromycin and Dactinomycin ingested with a blood meal depress the level of midgut trypsin. Trypsin purified from the midguts of A. aegypti fed upon defibrinated blood containing 14C-amino acids contains radioactivity. Midguts from blood-fed mosquitoes did not produce significant amounts of trypsin when dissected from mosquitoes and maintained in a tissue culture medium for up to 50 hr.

Type
Articles
Information
The Canadian Entomologist , Volume 105 , Issue 4 , April 1973 , pp. 599 - 603
Copyright
Copyright © Entomological Society of Canada 1973

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References

Akov, S. Protein digestion in haematophagous insects. Proc. Symposium on significance of insect and mite nutrition. Lexington, Ky. (In press.)Google Scholar
Fisk, F. W. and Shambaugh, G. F.. 1952. Protease activity in adult Aedes aegypti mosquitoes as related to feeding. Ohio J. Sci. 52: 80–88.Google Scholar
Gooding, R. H. 1966 a. In vitro properties of proteinases in the midgut of adult Aedes aegypti L. and Culex fatigans (Wiedemann). Comp. Biochem. Physiol. 17: 115–127.CrossRefGoogle ScholarPubMed
Gooding, R. H. 1966 b. Physiological aspects of digestion of the blood meal by Aedes aegypti (Linnaeus) and Culex fatigans Wiedemann. J. med. Ent. 3: 53–60.CrossRefGoogle ScholarPubMed
Gooding, R. H. 1968. Digestive proteinases from bloodsucking insects. Proc. Thirteenth int. Congr. Ent. pp. 381–382.Google Scholar
Gooding, R. H. 1969. Studies on proteinases from some bloodsucking insects. Proc. ent. Soc. Ont. 100: 139–145.Google Scholar
Gooding, R. H. 1972. Digestive processes of haematophagous insects. I. A literature review. Quaest. ent. 8: 5–60.Google Scholar
Grace, T. D. C. 1962. Establishment of four strains of cells from insect tissues grown in vitro. Nature 195: 788–789.Google Scholar
C-T., Huang 1971. Vertebrate serum inhibitors of Aedes aegypti trypsin. Insect Biochem. 1: 27–38.Google Scholar
Hummel, C. W. 1959. A modified spectrophotometric determination of chymotrypsin, and thrombin. Can. J. Biochem. Physiol. 37: 1393–1399.CrossRefGoogle ScholarPubMed
Schwert, G. W. and Takenaka, Y.. 1955. A spectrophotometric determination of trypsin and chymotrypsin. Biochim. biophys. Acta 16: 570–575.Google Scholar
Service, M. W. 1968. Observations on feeding and oviposition in some British mosquitoes. Entomologia exp. appl. 11: 277–285.Google Scholar
Shambaugh, G. F. 1954. Protease stimulation by foods in adult Aedes aegypti Linn. Ohio J. Sci. 54: 151–160.Google Scholar
Yang, Y. J. and Davies, D. M.. 1971. Trypsin and chymotrypsin during metamorphosis in Aedes aegypti and properties of the chymotrypsin. J. Insect Physiol. 17: 117–131.Google Scholar