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A SIMPLE STAINING TECHNIQUE FOR ASSESSING FEEDING DAMAGE BY LEPTOGLOSSUS OCCIDENTALIS HEIDEMANN (HEMIPTERA: COREIDAE) ON CONES

Published online by Cambridge University Press:  31 May 2012

Bruce C. Campbell  and
Patrick J. Shea
Bruce C. Campbell
Affiliation:
U.S. Department of Agriculture, Agricultural Research Service, Western Regional Research Center, 800 Buchanan Street, Albany, California, USA 94710
Patrick J. Shea
Affiliation:
U.S. Department of Agriculture, Forest Service, Pacific Southwest Research Station, PO Box 245, Berkeley, California, USA 94701
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Abstract

Pectinmethylesterase (PME) activity was found in the salivary glands of nymphs and adults of a leaf-footed bug, Leptoglossus occidentalis Heidemann. Puncture wounds in cone scales resulting from PME activity in the saliva of these bugs were seen by staining with a 0.05% aqueous solution of ruthenium red. This staining technique can be used to estimate feeding damage by L. occidentalis on cones of western white pine (Pinus monticola Dougl.), sugar pine (P. lambertiana Dougl.), and Douglas-fir (Pseudotsuga menziesii [Mirb.] Franco).

Résumé

L’activité de pectineméthylestérase (PME) a été trouvée dans les glandes salivaires des nymphes et des adultes du pentatome à pied-feuillu, Leptoglossus occidentalis Heidemann. Les blessures de perforation des écailles des cônes survenant de l’activité de la PME de la salive de ces pentatomes ont été démontrées par teinture avec une solution aqueuse de 0,05% de rouge du ruthénium. Cette technique de teinture peut être employée pour juger le dommage dû à l’alimentation de L. occidentalis sur les cônes du pin blanc occidental (Pinus monticola Dougl.), du pin à sucre (P. lambertiana Dougl.) et le sapin Douglas (Pseudotsuga menziesii [Mirb.] Franco).

Type
Articles
Information
The Canadian Entomologist , Volume 122 , Issue 5 , October 1990 , pp. 963 - 968
Copyright
Copyright © Entomological Society of Canada 1990

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