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Pre-release detection of a biocontrol agent: combining independent and public DNA sequences to identify the first North American record of Aulacidea pilosellae (Hymenoptera: Cynipidae)

Published online by Cambridge University Press:  11 August 2014

Chandra E. Moffat  and
M. Alex Smith
Chandra E. Moffat*
Affiliation:
Department of Biology, University of British Columbia, Okanagan Campus, 3333 University Way, Kelowna, British Columbia, Canada
M. Alex Smith
Affiliation:
Department of Integrative Biology, University of Guelph, Guelph, Ontario, Canada
*
2 Corresponding author (e-mail: [email protected]).
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Abstract

Here we report the first North American detection of the gall wasp Aulacidea pilosellae Kieffer (Hymenoptera: Cynipidae), native to Central Europe and a promising candidate biological control agent for invasive hawkweeds (Pilosella Vaillant, Asteraceae) in North America. This occurrence was discovered through the intersection of (i) publically available DNA barcode data and (ii) DNA sequencing of a biocontrol agent before its release. COI DNA sequences of A. pilosellae collected in Central Europe were compared with publically available DNA sequence records. Despite the presence of other Aulacidea Ashmead in the database, the most similar sequence to the European A. pilosellae was an as yet unidentified specimen collected in the Ottawa Valley of eastern Ontario, Canada. Subsequent sequencing of a second gene region (28S-D2) of the Ottawa Valley specimen yielded an identical DNA sequence to the European A. pilosellae, confirming the presence of this species in Ontario. This note highlights the potential synergy that can result from making DNA barcode data publically available before formal taxonomic identification, and a new benefit of incorporating DNA sequencing of standardised markers into biological control programmes.

Type
Physiology, Biochemistry, Development and Genetics – NOTE
Information
The Canadian Entomologist , Volume 147 , Issue 4 , August 2015 , pp. 390 - 395
Copyright
© Entomological Society of Canada 2014 

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Footnotes

Subject Editor: Chris Schmidt

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