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PCR-based methods for identification of two Eteobalea species (Lepidoptera: Cosmopterigidae) used as biocontrol agents of weedy Linaria species (Scrophulariaceae)

Published online by Cambridge University Press:  02 April 2012

Andrew Mitchell ,
Alec S. McClay ,
Gregory R. Pohl  and
Felix A.H. Sperling
Andrew Mitchell*
Affiliation:
Agricultural Scientific Collections Unit, Orange Agricultural Institute, NSW Department of Primary Industries, Forest Road, Orange, NSW 2800, Australia
Alec S. McClay
Affiliation:
McClay Ecoscience, 15 Greenbriar Crescent, Sherwood Park, Alberta, Canada T8H 1H8
Gregory R. Pohl
Affiliation:
Natural Resources Canada, Canadian Forest Service, Northern Forestry Centre, 5320 – 122 Street, Edmonton, Alberta, Canada T6H 3S5
Felix A.H. Sperling
Affiliation:
Department of Biological Sciences, CW 405, Biological Sciences Centre, University of Alberta, Edmonton, Alberta, Canada T6G 2E9
*
1Corresponding author (e-mail: [email protected]).
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Abstract

Two European species of the genus Eteobalea Hodges are being tested as classical biological control agents for toadflax (Linaria spp.) in Canada. Permits for the release of these species apply only to an Italian population of E. serratella Treitschke and a Serbian population of E. intermediella Riedl. When these species are imported as larvae or pupae from an area where they are sympatric, such as around Rome, they must later be separated in the laboratory. This step is essential to establish a pure laboratory colony of the correct species, both to increase the rate of breeding success and to comply with conditions of the importation permit. Although the male and female genitalia and the egg chorion have good diagnostic characters, these characters are not useful for identifying immature stages or newly emerged adults before mating and oviposition. This problem has hampered the establishment of rearing colonies of these two species in Canada. We describe the development and application of four polymerase chain reaction – restriction fragment length polymorphism assays that have been used to distinguish between these two species. If and when either of these species becomes established in the field, the tests presented here should also prove useful for monitoring populations.

Résumé

Deux espèces européennes d'Eteobalea Hodges sont actuellement à l'essai au Canada comme des agents de lutte biologique contre les mauvaises herbes du genre Linaria. Les permis pour le lâcher de ces espèces sont valables seulement pour une population italienne d'E. serratella Treitschke et une population serbienne d'E. intermediella Riedl. Lorsque ces espèces sont importées d'une région où elles existent en sympatrie, par exemple près de Rome, elles doivent être séparées plus tard dans le laboratoire. Ceci est indispensable pour établir une colonie pure de l'espèce correcte, à la fois pour assurer le succès de la multiplication et pour se conformer aux conditions du permis d'importation. Bien que les organes génitaux des deux sexes et le chorion de l'oeuf possèdent des caractères utiles pour leur identification, ceux-ci ne servent pas pour l'identification des stades immatures, ni des adultes nouvellement émergés avant de l'accouplement et l'oviposition. Ceci a entravé l'établissement des colonies de multiplication de ces deux espèces d'Eteobalea au Canada. Nous décrivons le développement et l'application de quatre essais de réaction de polymérisation en chaîne – polymorphismes de longueur de fragments de restriction qui ont été utilisés pour distinguer entre ces espèces. Ces essais seront également utiles pour la surveillance des populations dans le cas que n'importe quelle de ces espèces s'établit dans le champ.

Type
Articles
Information
The Canadian Entomologist , Volume 137 , Issue 2 , April 2005 , pp. 129 - 137
Copyright
Copyright © Entomological Society of Canada 2005

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