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Molecular characterization of the entomopathogenic fungi Lecanicillium spp. (Deuteromycota: Hyphomycetes) isolated from white pine weevil, Pissodes strobi (Coleoptera: Curculionidae), in British Columbia

Published online by Cambridge University Press:  02 April 2012

Isabel Leal ,
René I. Alfaro ,
Young Woon Lim  and
Harry H. Kope
Isabel Leal*
Affiliation:
Pacific Forestry Centre, Canadian Forest Service, Victoria, British Columbia, Canada V8Z 1M5
René I. Alfaro
Affiliation:
Pacific Forestry Centre, Canadian Forest Service, Victoria, British Columbia, Canada V8Z 1M5
Young Woon Lim
Affiliation:
National Institute of Biological Resources, Gyungseo-dong, Seo-gu, Incheon 404–708, Korea
Harry H. Kope
Affiliation:
Forest Practices Branch, British Columbia Ministry of Forests and Range, Victoria, British Columbia, Canada V8W 1R8
*
1Corresponding author (e-mail: [email protected]).
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Abstract

The entomopathogenic fungal genus Lecanicillium Gams and Zare includes species that are highly pathogenic to many genera of insects. Three species, Lecanicillium longisporum (Petch) Zare and W. Gams, L. muscarium (Petch) Zare and W. Gams, and L. pissodis Kope and Leal, were found to be entomopathogens of adult white pine weevils, Pissodes strobi (Peck), in coastal British Columbia. Morphological characteristics were used to identify these species, but variation in conidial shape and size made it difficult to classify some of the isolates into the correct species of Lecanicillium. To confirm the identity of these Lecanicillium species, we used molecular tools such as polymerase chain reaction – restriction fragment length polymorphism and DNA sequencing.

Résumé

Le genre de champignons entomopathogènes Lecanicillium Gams et Zare contient des espèces qui sont fortement pathogènes pour de nombreuses catégories d’insectes. Trois espèces, Lecanicillium longisporum (Petch) Zare et W. Gams, L. muscarium (Petch) Zare et W. Gams et L. pissodis Kope et Leal, sont des entomopathogènes des adultes du charançon du pin blanc, Pissodes strobi (Peck), dans la région côtière de la Colombie-Britannique. Les caractéristiques morphologiques nous ont servi à identifier ces espèces, mais la variabilité de la forme et de la taille des conidies rend difficile le rattachement de certains de ces isolats à la bonne espèce de Lecanicillium. Afin de confirmer l’identité de ces espèces de Lecanicillium, nous avons utilisé des outils moléculaires, tels que la réaction de polymérisation en chaîne – polymorphisme de la longueur des fragments de restriction et le séquençage de l’ADN.

[Traduit par la Rédaction]

Type
Articles
Information
The Canadian Entomologist , Volume 140 , Issue 2 , April 2008 , pp. 168 - 173
Copyright
Copyright © Entomological Society of Canada 2008

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References

Alfaro, R.I. 1994. The white pine weevil in British Columbia: biology and damage. In The white pine weevil: biology, damage and management. Edited by Alfaro, R.I., Kiss, G., and Fraser, R.G.. Forest Resources Development Agreement (FRDA) Rep. No. 226. pp. 7–22.Google Scholar
Alfaro, R.I., Hulme, M.A., and Harris, J.W.E. 1985. Insects associated with the Sitka spruce weevil, Pissodes strobi (Coleoptera: Curculionidae), on Sitka spruce, Picea sitchensis, in British Columbia, Canada. Entomophaga, 32: 415–418.CrossRefGoogle Scholar
Alfaro, R.I., Borden, J.H., Fraser, R.G., and Yanchuk, A. 1995. The white pine weevil in British Columbia: basis for an integrated pest management system. Forestry Chronicle, 71: 66–73.CrossRefGoogle Scholar
Bruns, T.D., Vilgalys, R., Barns, S.M., Gonzalez, D., Hibbett, D.S., Lane, D.J., Simon, L., Stickel, S., Szaro, T.M., Weisberg, W.G., and Sogin, M.L. 1992. Evolutionary relationships within the fungi: analysis of nuclear small subunit rRNA sequences. Molecular Phylogenetics and Evolution, 1: 231–241.CrossRefGoogle ScholarPubMed
Fraaije, B.A., Lowell, D.J., Coelho, J.M., Baldwin, S., and Hollomon, D.W. 2001. PCR-based assays to assess wheat varietal resistance to blotch (Septoria tritici and Stagonospora nodorum) and rust (Puccinia striiformis and Puccinia recondita) diseases. European Journal of Plant Pathology, 107: 905–917.CrossRefGoogle Scholar
Glass, N.L., and Donaldson, G.C. 1995. Development of primer sets designed for use with PCR to amplify conserved genes from filamentous ascomycetes. Applied and Environmental Microbiology, 31: 1323–1330.CrossRefGoogle Scholar
Harrington, T.C., McNew, D., Steinel, J., Hofstra, D., and Farrel, R. 2001. Phylogeny and taxonomy of the Ophiostoma piceae complex and the Dutch elm disease fungi. Mycologia, 93: 111–136.CrossRefGoogle Scholar
Hermosa, M.R., Grondona, I., Monte, E., Iturriaga, E.A., Diaz-Minguez, J.M., Castro, C., Monte, E., and Garcia-Ach, J.M. 2000. Molecular characterization and identification of biocontrol isolates of Trichoderma spp. Applied and Environmental Microbiology, 66: 1890–1898.CrossRefGoogle ScholarPubMed
Jacobs, K., Wingfield, M.J., and Wingfield, B.D. 2001. Phylogenetic relationship in Leptographium based on morphological and molecular characters. Canadian Journal of Botany, 79: 719–732.CrossRefGoogle Scholar
Kim, J.J., Kim, S.H., Lee, S., and Breuil, C. 2003. Distinguishing Ophiostoma ips and Ophiostoma montium, two bark beetle-associated sapstain fungi. FEMS Microbiology Letters, 222: 187–192.CrossRefGoogle ScholarPubMed
Kim, J.J., Lim, Y.W., Wingfield, M.J., Breuil, C., and Kim, G.H. 2004. Leptographium bistatum sp. nov., a new species with a Sporothrix synanamorph from Pinus radiata in Korea. Mycological Research, 108: 699–706.CrossRefGoogle Scholar
Kope, H.H., and Leal, I. 2005. A new species of Lecanicillium isolated from the white pine weevil, Pissodes strobi. Mycotaxon, 94: 331–340.Google Scholar
Kope, H.H., Peterson, M., and Alfaro, R. 2000. Pathogenicity of Verticillium lecanii to Pissodes strobi. Canadian Journal of Plant Pathology, 22: 187. [Abstr.]CrossRefGoogle Scholar
Kope, H.H., Alfaro, R., and Lavallée, R. 2006. Virulence of the entomopathogenic fungus Lecanicillium (Deuteromycota: Hyphomycetes) to Pissodes strobi (Coleoptera: Curculionidae). The Canadian Entomologist, 138: 253–262.CrossRefGoogle Scholar
McKay, G.J., Brown, A.E., Bjourson, A.J., and Mercer, P.C. 1999. Molecular characterization of Alternaria linicola and its detection in linseed. European Journal of Plant Pathology, 105: 157–166.CrossRefGoogle Scholar
White, T.J., Bruns, T., Lee, S., and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR protocols: a guide to methods and applications. Edited by Innis, M.A. et al. Academic Press Inc., New York. pp. 315–322.Google Scholar
Zare, R., and Gams, W. 2001. A revision of Verticillium section Prostrata. IV. The genera Lecanicillium and Simplicillium gen. nov. Nova Hedwigia, 73: 1–50.CrossRefGoogle Scholar