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ENZYME POLYMORPHISM IN THE CORN EARWORM, HELIOTHIS ZEA (LEPIDOPTERA: NOCTUIDAE). HEMOLYMPH ESTERASE POLYMORPHISM

Published online by Cambridge University Press:  31 May 2012

Douglas K. Sell
Affiliation:
Illinois Natural History Survey and Illinois Agricultural Experiment Station, Urbana
Gregory S. Whitt
Affiliation:
Illinois Natural History Survey and Illinois Agricultural Experiment Station, Urbana
Robert L. Metcalf
Affiliation:
Illinois Natural History Survey and Illinois Agricultural Experiment Station, Urbana
Lu-ping Kan Lee
Affiliation:
Illinois Natural History Survey and Illinois Agricultural Experiment Station, Urbana

Abstract

Esterases present in the hemolymph of last instar Heliothis zea (Boddie) were separated by polyacrylamide gel electrophoresis. Four systems, all polymorphic, were observed. One system, Est-II, appeared especially suited for the study of some of the important population processes of this species; these include host–plant relations, migration, and the effects of environmental pressures such as pesticides on the species.

Est-II was classified as a carboxylesterase, being sensitive only to organophosphate inhibitors. Dichlorvos at 10−5M completely inhibited Est-II activity.

Populations collected from corn from Tifton, Ga., and in two locations in Illinois, at approximately the same time were very similar genetically when compared on the basis of Est-II allele frequencies. A population collected from leguminous hosts in Tifton, Ga., at the same time as the above three populations was different genetically.

Est-IIc, an allele normally rare or absent in the populations studied, was present at a high frequency in one Urbana population collection. Est-IIc may be useful in studying the long-range dispersal of H. zea.

Type
Articles
Copyright
Copyright © Entomological Society of Canada 1974

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