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Using comparative genomics to develop a molecular diagnostic for the identification of an emerging pest Drosophila suzukii

Published online by Cambridge University Press:  25 March 2015

K.A. Murphy ,
T.R. Unruh ,
L.M. Zhou ,
F.G. Zalom ,
P.W. Shearer ,
E.H. Beers ,
V.M. Walton ,
B. Miller  and
J.C. Chiu
K.A. Murphy
Affiliation:
Department of Entomology and Nematology, College of Agricultural and Environmental Sciences, University of California, Davis, CA 95616, USA
T.R. Unruh
Affiliation:
USDA-ARS, Wapato, WA 98951, USA
L.M. Zhou
Affiliation:
Department of Entomology and Nematology, College of Agricultural and Environmental Sciences, University of California, Davis, CA 95616, USA
F.G. Zalom
Affiliation:
Department of Entomology and Nematology, College of Agricultural and Environmental Sciences, University of California, Davis, CA 95616, USA
P.W. Shearer
Affiliation:
Mid-Columbia Agricultural and Extension Center, Oregon State University, Hood River, OR 97031, USA
E.H. Beers
Affiliation:
Tree Fruit Research and Extension Center, Washington State University, Wenatchee, WA 98801, USA
V.M. Walton
Affiliation:
Department of Horticulture, Oregon State University, Corvallis, OR 97331, USA
B. Miller
Affiliation:
Department of Horticulture, Oregon State University, Corvallis, OR 97331, USA
J.C. Chiu*
Affiliation:
Department of Entomology and Nematology, College of Agricultural and Environmental Sciences, University of California, Davis, CA 95616, USA
*
*Author for correspondence Phone: (530) 752-1839 Fax: (530) 754-9077 E-mail: [email protected]
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Abstract

Drosophila suzukii (Spotted Wing Drosophila) has recently become a serious invasive pest of fruit crops in the USA, Canada, and Europe, leading to substantial economic losses. D. suzukii is a direct pest, ovipositing directly into ripe or ripening fruits; in contrast, other Drosophilids utilize decaying or blemished fruits and are nuisance pests at worst. Immature stages of D. suzukii are difficult to differentiate from other Drosophilids, posing problems for research and for meeting quarantine restrictions designed to prevent the spread of this pest in fruit exports. Here we used a combined phylogenetic and bioinformatic approach to discover genetic markers suitable for a species diagnostic protocol of this agricultural pest. We describe a molecular diagnostic for rapid identification of single D. suzukii larva using multiplex polymerase chain reaction. Our molecular diagnostic was validated using nine different species of Drosophila for specificity and 19 populations of D. suzukii from different geographical regions to ensure utility within species.

Keywords

spotted wing drosophilaDrosophila suzukiispecies-specific PCRinvasive pestcomparative genomics
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 105 , Issue 3 , June 2015 , pp. 364 - 372
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Copyright
Copyright © Cambridge University Press 2015 

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