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Use of the random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) to detect DNA polymorphisms in aphids (Homoptera: Aphididae)

Published online by Cambridge University Press:  10 July 2009

William C. Black IV ,
Nancy M. DuTeau ,
Gary J. Puterka ,
James R. Nechols  and
Jennifer M. Pettorini
William C. Black IV*
Affiliation:
Department of Entomology, Kansas State University, Manhattan, KS 66506, USA
Nancy M. DuTeau
Affiliation:
Department of Plant Pathology, Kansas State University, Manhattan, KS 66506, USA
Gary J. Puterka*
Affiliation:
USDA-ARS, Plant Science Research Laboratory, 1301 N. Western, Stillwater, OK 74075, USA
James R. Nechols
Affiliation:
Department of Entomology, Kansas State University Manhattan, KS 66506, USA
Jennifer M. Pettorini
Affiliation:
Department of Entomology, Kansas State University Manhattan, KS 66506, USA
*
Dr William C. Black IV, Department of Environmental Health, Colorado State University, Fort Collins, Colorado 80525, USA.
USDA-ARS, Appalachian Fruit Research Station, 45 Wiltshire Road, Kearneysville, WV 25430, USA.
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Abstract

We have used a new technique to identify discrete genetic markers in aphids, a family in which biochemical and morphological genetic polymorphisms are rare. The new technique uses the polymerase chain reaction (PCR) to amplify random regions of aphid genomes (random amplified polymorphic DNA) and has been termed RAPD-PCR. We demonstrate the use of the technique in revealing genetic variation in four aphid species, the greenbug (Schizaphis graminum (Rondani)), the Russian wheat aphid (Diuraphis noxia (Mordvilko)), the pea aphid (Acyrthosiphon pisum (Harris)), and the brown ambrosia aphid (Uroleucon ambrosiae (Thomas)). In contrast with allozyme surveys, RAPD-PCR revealed large amounts of genetic variation among individuals in each of these species. Variation was detected among biotypes, populations, colour morphs and even individuals on a single plant. We also explored the utility of RAPD-PCR in the detection and identification within aphid bodies of two endoparasitic wasps, Diaeretiella rapae (McIntosh) and Lysiphlebus testaceipes (Cresson). The use of RAPD-PCR in species diagnostics, parasitoid detection, and population studies is discussed.

Type
Research Paper
Information
Bulletin of Entomological Research , Volume 82 , Issue 2 , June 1992 , pp. 151 - 159
Copyright
Copyright © Cambridge University Press 1992

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