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The use of serology in evaluating invertebrate prey-predator relationships : a review

Published online by Cambridge University Press:  10 July 2009

P. F. L. Boreham  and
C. E. Ohiagu
P. F. L. Boreham*
Affiliation:
Department of Zoology and Applied Entomology, Imperial College of Science and Technology, Prince Consort Road, London, SW7 2AZ, UK
C. E. Ohiagu
Affiliation:
Department of Zoology and Applied Entomology, Imperial College of Science and Technology, Prince Consort Road, London, SW7 2AZ, UK
*
*Address for correspondence: Imperial College Field Station, Silwood Park, Ascot, Berks.
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Abstract

Several methods, including the use of serology, are available for evaluating prey-predator relationships. Two basic methods involving precipitation and agglutination reactions have been utilised, both of which depend on the production of specific antisera with high titres. A critical account is presented of methods of preparing antisera, with particular reference to the amount of antigen required, the route of inoculation and testing antisera, together with a review of the five precipitation and two agglutination techniques available. The main limitations of serological methods are tje specificity of the arrtisera and the difficulties in quantifying results unless assumptions are made as to the number of prey eaten at each feed. Details of studies employing serological techniques to identify the predators of specific prey and the food od invertebrates are given intwo tables. The value of serological techniques is discussed in relation to the ecology of invertebrates by considering, as examples, te insect fauna on broon (Sarothamnus scoparius), predation of mosquitoes and the food of triclads. Errors in the quantification of predation may occur by including positive reactions resulting from carrion feeding or material ingested acidentally on plants by phytophagous arthropods.

Type
Review Article
Information
Bulletin of Entomological Research , Volume 68 , Issue 2 , July 1978 , pp. 171 - 194
Copyright
Copyright © Cambridge University Press 1978

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