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Susceptibility baselines for DDT metabolism and related enzyme systems in the sandfly Phlebotomus papatasi (Scopoli) (Diptera: Psychodidae)

Published online by Cambridge University Press:  10 July 2009

S. El-Sayed ,
J. Hemingway  and
R. P. Lane
S. El-Sayed
Affiliation:
School of Hygiene & Environmental StudiesP.O. Box 205, Khartoum, Sudan
J. Hemingway
Affiliation:
Department of Medical Parasitology, London School of Hygiene & Tropical MedicineLondon, WCIE 7HT, UK
R. P. Lane*
Affiliation:
Department of Medical Parasitology, London School of Hygiene & Tropical MedicineLondon, WCIE 7HT, UK
*
*For correspondence
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Abstract

DDT metabolism in Phlebotomus papatasi (Scopoli) was investigated and compared to that in DDT-resistant and susceptible strains of Culex quinquefasciatus Say and Anopheles gambiae Giles with the objective of establishing baselines for sandfly studies. P. papatasi produced eight metabolites of DDT, with DDE predominating, as in the two mosquito species. Both oxidases and glutathione transferases were found to be involved in DDT metabolism in insecticide-susceptible adults of P. papatasi. The activity level of glutathione transferases and the reduced and oxidized difference spectra of cytochrome P-450 were measured spectrophotometrically. The level of glutathione transferase activity in P. papatasi was lower than that in susceptible C. quinquefasciatus adults when expressed in terms of the activity per milligram of soluble protein but, in contrast, the cytochrome P-450 was slightly higher in both the reduced and oxidized states.

Type
Original Articles
Information
Bulletin of Entomological Research , Volume 79 , Issue 4 , November 1989 , pp. 679 - 684
Copyright
Copyright © Cambridge University Press 1989

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