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The silkworm GSTe4 is sensitive to phoxim and protects HEK293 cells against UV-induced cell apoptosis

Published online by Cambridge University Press:  08 April 2015

X.Y. Chen ,
J. Liu ,
C.D. Zhang ,
Y.F. Li ,
T.H. Liu ,
L. Wang ,
Q.Y. Yu ,
Y.H. Zhang ,
C. Lu  and
M.H. Pan
X.Y. Chen
Affiliation:
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China
J. Liu
Affiliation:
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China The management of experimental teaching center, ChongQing Medical University, Chongqing 400016, China
C.D. Zhang
Affiliation:
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China Department of Biochemistry and Molecular Biology, ChongQing Medical University, Chongqing 400016, China
Y.F. Li
Affiliation:
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China
T.H. Liu
Affiliation:
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China
L. Wang
Affiliation:
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China
Q.Y. Yu
Affiliation:
The Institute of Agricultural and Life Sciences, Chongqing University, Chongqing 400044, China
Y.H. Zhang
Affiliation:
The Sericultural Research Institute, Sichuan Academy of Agricultural Science, Sichuan 637000, China
C. Lu
Affiliation:
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing 400716, China
M.H. Pan*
Affiliation:
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing 400716, China
*
*Author for correspondence Phone: +86 23 68250793 Fax: +86 23 68251128 E-mail: [email protected]
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Abstract

Glutathione S-transferases (GSTs, EC 2.5.1.18) are a family of super enzymes with multiple functions that play a major role in the detoxification of endogenous and xenobiotic compounds. In our previous study, we have predicted 23 putative cytosolic GSTs in the silkworm genome using bioinformatic methods. In this study, we cloned and studied the insect-specific epsilon-class GST gene GSTe4 from the silkworm, Bombyx mori. The recombinant BmGSTe4 (Bac-BmGSTe4) was overexpressed in SF-9 cell lines, and it was found to have effective GST activity. We also found that the expression of BmGSTe4 was especially down-regulated after the silkworms were fumigated with or ingested phoxim. Moreover, BmGSTe4 protected HEK293 cells against UV-induced cell apoptosis. These results demonstrated that BmGSTe4 has GST activity, is sensitive to phoxim, and plays a role in inhibition of UV-induced cell apoptosis.

Keywords

glutathione S-transferasefunctionsensitive to phoximcell apoptosis
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 105 , Issue 4 , August 2015 , pp. 399 - 407
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Copyright
Copyright © Cambridge University Press 2015 

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