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Screening of entomopathogenic nematodes for virulence against the invasive western corn rootworm, Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae) in Europe

Published online by Cambridge University Press:  09 March 2007

S. Toepfer ,
C. Gueldenzoph ,
R.-U. Ehlers  and
U. Kuhlmann
S. Toepfer
Affiliation:
CABI Bioscience Switzerland Centre, Rue des Grillons 1, 2800 Delémont, Switzerland
C. Gueldenzoph
Affiliation:
CABI Bioscience Switzerland Centre, Rue des Grillons 1, 2800 Delémont, Switzerland
R.-U. Ehlers
Affiliation:
Institute for Phytopathology, Christian-Albrechts-University Kiel, Klausdorfer Street 28, 24223 Raisdorf, Germany
U. Kuhlmann*
Affiliation:
CABI Bioscience Switzerland Centre, Rue des Grillons 1, 2800 Delémont, Switzerland
*
*Fax: +0041 32 4214871 E-mail: [email protected]
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Abstract

Entomopathogenic nematode species available in Europe were screened for their efficacy against both the root-feeding larvae and silk-feeding adults of the western corn rootworm, Diabrotica virgifera virgifera LeConte. Laboratory screening tests were aimed at the selection of candidate biological control agents for the management of this invasive alien pest in Europe. Steinernema glaseri, S. arenarium, S. abassi, S. bicornutum, S. feltiae, S. kraussei, S. carpocapsae and Heterorhabditis bacteriophora were studied to determine their virulence against third instar larvae and adults of D. v. virgifera in small-volume arenas (using nematode concentrations of 0.5, 0.8, 7.9 and 15.9 infective juveniles cm–2). All nematode species were able to invade and propagate in D. v. virgifera larvae, but adults were rarely infected. At concentrations of 7.9 and 15.9 cm–2, S. glaseri, S. arenarium, S. abassi and H. bacteriophora caused the highest larval mortality of up to 77%. Steinernema bicornutum, S. abassi, S. carpocapsae and H. bacteriophora appeared to have a high propagation level, producing 5970±779, 5595±811, 5341±1177 and 4039±1025 infective juveniles per larva, respectively. Steinernema glaseri, S. arenarium, S. feltiae, S. kraussei and H. bacteriophora were further screened at a concentration of 16.7 nematodes cm–2 against third instar larvae in medium-volume arenas (sand-filled trays with maize plants). Heterorhabditis bacteriophora, S. arenarium and S. feltiae caused the highest larval mortality with 77±16.6%, 67±3.5%, and 57±17.1%, respectively. In a next step, criteria for rating the entomopathogenic nematode species were applied based on results obtained for virulence and propagation, and for current production costs and availability in Europe. These criteria were then rated to determine the potential of the nematodes for further field testing. Results showed the highest potential in H. bacteriophora, followed by S. arenarium and S. feltiae, for further testing as candidate biological control agents.

Keywords

Diabrotica virgifera virgiferaSteinernemaHeterorhabditisentomopathogenic nematodesEuropebiological controlvirulencescreening
Type
Research Article
Information
Bulletin of Entomological Research , Volume 95 , Issue 5 , October 2005 , pp. 473 - 482
Copyright
Copyright © Cambridge University Press 2005

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References

Baca, F. (1994) New member of the harmful entomofauna of Yugoslavia Diabrotica virgifera virgifera LeConte (Coleoptera, Chrysomelidae). Zastita Bilja 45, 125–131.Google Scholar
Barbercheck, M.E. (1993) Tritrophic level effects on entomopathogenic nematodes. Environmental Entomology 22, 1166–1171.CrossRefGoogle Scholar
Barbercheck, M.E., Warrick, W.C. Jr. (1997) Evaluation of trap cropping and biological control against southern corn rootworm (Coleoptera: Chrysomelidae) in peanuts. Journal of Entomological Science 32, 229–243.CrossRefGoogle Scholar
Baufeld, P. & Enzian, S. (2005) Maize high-risk areas and potential yield losses due to western corn rootworm (Diabrotica. v. virgifera LeConte) damage in selected European countries. pp. 285–302Vidal, S.Kuhlmann, U.Edwards, C.R. (Eds) Western corn rootworm: ecology and management. Wallingford, Oxon, CABI Publishing.CrossRefGoogle Scholar
Brust, G.E. (1991) Augmentation of an endemic entomogenous nematode by agroecosystem manipulation for the control of a soil pest. Agriculture Ecosystems and Environment 36, 175–184.CrossRefGoogle Scholar
Chiang, H.C. (1973) Bionomics of the northern and western corn rootworms. Annual Review of Entomology 18, 47–72.CrossRefGoogle Scholar
Ehlers, R.U. (2001) Mass production of entomopathogenic nematodes for plant protection. Applied Microbiology and Biotechnology 56, 623–633.CrossRefGoogle ScholarPubMed
Ehlers, R.U. (2003) Entomopathogenic nematodes in the European biocontrol market. Communications in Agriculture and Applied Biological Sciences 68, 3–16.Google ScholarPubMed
Elsey, K.D. (1977) Parasitism of some economically important species of Chrysomelidae by nematodes of the genus Howardula. Journal of Invertebrate Pathology 29, 384–385.CrossRefGoogle Scholar
Gaugler, R. (1981) Biological potential of neoplectanid nematodes. Journal of Nematology 14, 126–131.Google Scholar
Gaugler, R. (2002) Entomopathogenic nematology. Wallingford, Oxon, CABI Publishing.CrossRefGoogle Scholar
Georgis, R., Kaya, H.K. & Gaugler, R. (1991) Effect of steinernematid and Heterorhabditis nematodes (Rhabditida: Steinernematidae and Heterorhabditidae) on non-target arthropods. Environmental Entomology 20, 814–822.CrossRefGoogle Scholar
Gouge, D.H., Shapiro-Ilan, D.I. (2003) Case studies in cotton and citrus: use of entomopathogenic nematodes. Indian Journal of Nematology 33, 91–102.Google Scholar
Jackson, J.J. (1996) Field performance of entomopathogenic nematodes for suppression of western corn rootworm (Coleoptera: Chrysomelidae). Journal of Economic Entomology 89, 366–372.CrossRefGoogle Scholar
Jackson, J.J., Brooks, M.A. (1989) Susceptibility and immune response of western corn rootworm larvae (Coleoptera: Chrysomelidae) to the entomogenous nematode, Steinernema feltiae (Rhabditida: Steinernematidae). Journal of Economic Entomology 82, 1073–1077.CrossRefGoogle Scholar
Jackson, J.J. & Brooks, A. (1995) Parasitism of western corn rootworm larvae and pupae by Steinernema carpocapsae. Journal of Nematology 27, 15–20.Google ScholarPubMed
Jackson, J.J., Hesler, S.L. (1996) Placement and application rate of the nematode Steinernema carpocapsae (Rhabditida: Steinernematidae) for suppression of the western corn rootworm (Coleoptera: Chrysomelidae). Journal of the Kansas Entomological Society 68, 461–467.Google Scholar
Journey, A.M., Ostlie, K.R. (2000) Biological control of the western corn rootworm (Coleoptera: Chrysomelidae) using the entomopathogenic nematode, Steinernema carpocapsae. Environmental Entomology 29, 822–831.CrossRefGoogle Scholar
Kaya, H.K., Stock, S.P. (1997) Techniques in insect nematology. pp. 281–324 in Lacey, L.A. (ed.) Manual of techniques in insect pathology. London, Academic Press.CrossRefGoogle Scholar
Kinnear, P.R., Gray, C.D. (2000) SPSS for windows made simple East Sussex, Psychology Press.Google Scholar
Kiss, J., Edwards, C.R., Berger, H., Cate, P., Cean, M., Cheek, S., Derron, J., Festic, H., Furlan, L., Igrc-Barcic, J., Lammers, W., Omelyuta, V., Princzinger, G., Reynaud, P., Sivcev, I., Sivicek, P., Urek, G. & Vahala, O. (2005) Monitoring of western corn rootworm (Diabrotica v. virgifera LeConte) in Europe 1992–2003 Western corn rootworm: ecology and management 29–40Vidal, S., Kuhlmann, U., Edwards, C.R.Wallingford, Oxon Published on behalf of The Nutrition Society by CABI PublishingGoogle Scholar
Krysan, J.L., Miller, T.A. (1986) Methods of study for pest Diabrotica. New York, Springer.CrossRefGoogle Scholar
Kuhlmann, U., van der, Burgt W.A.C.M. (1998) Possibilities for biological control of the western corn rootworm, Diabrotica virgifera virgifera LeConte, in Central Europe. Biocontrol News and Information 19, 59–68.Google Scholar
Kuhlmann, U., Toepfer, S. & Zhang, F. (2005) Is classical biological control against western corn rootworm in Europe a potential sustainable management strategy? pp. 263–284in Vidal, S., Kuhlmann, U., Edwards, C.R. (Eds) Western corn rootworm: ecology and management. Wallingford, Oxon, CABI Publishing.CrossRefGoogle Scholar
Levine, E., Oloumi, S.H. (1991) Management of diabroticite rootworms in corn. Annual Review of Entomology 36, 229–255.CrossRefGoogle Scholar
Nickle, W.R., Connick, W.J., Cantelo, W.W. Jr. (1994) Effects of pesta-pelletized Steinernema carpocapsae (All) on western corn rootworms and Colorado potato beetles. Journal of Nematology 26, 249–250.Google ScholarPubMed
Peters, A., Huneke, K., Ehlers, R.U. (1996) Host finding by the entomopathogenic nematode Steinernema feltiae. IOBC/WPRS Bulletin 19, 99–102.Google Scholar
Poinar, G.O. Jr., Evans, J.S., & Schuster, E. (1983) Field test of the entomogenous nematode, Neoaplectana carpocapsae, for control of corn rootworm larvae (Diabrotica sp., Coleoptera). Protection Ecology 5, 337–342.Google Scholar
Riga, E., Wistlecraft, J. & Potter, J. (2001) Potential of controlling insect pests of corn using entomopathogenic nematodes. Canadian Journal of Plant Sciences 81, 783–787.CrossRefGoogle Scholar
Singh, P., Moore, R.F. (1985) Handbook of insect rearing. Amsterdam, Elsevier Science.Google Scholar
Sivcev, I., Manojlovic, B., Krnjajic, S., Dimic, N., Draganic, M., Baca, F., Kaitovic, Z., Sekulic, R. & Keresi, T. (1994) Distribution and harmful effect of Diabrotica virgifera LeConte (Coleoptera, Chrysomelidae), a new maize pest in Yugoslavia. Zastita Bilja 45, 19–26.Google Scholar
Thurston, G.S., Yule, W.N. (1990) Control of larval northern corn rootworm (Diabrotica barberi) with two steinernematid nematode species. Journal of Nematology 22, 127–131.Google ScholarPubMed
Toepfer, S. & Kuhlmann, U. (2004a) IPM strategies for the western corn rootworm (Coleoptera: Chrysomelidae): current status and potential adoptations to the European maize productions system. Mitteilungen der Deutschen Gesellschaft für Allgemeine und Angewandte Entomologie 14, 391–398.Google Scholar
Toepfer, S. & Kuhlmann, U. (2004b) Search for natural enemies of the invasive alien chrysomelid, Diabrotica virgifera virgifera, in Central Europe. BioControl 49, 385–395.CrossRefGoogle Scholar
van der Burgt, W.A.C.M., Budai, C., Fodor, A., Lucskai, A. (1998) Parasitism of western corn rootworm adults by Steinernema spp. and Heterorhabditis spp. Proceedings of the Society for Experimental and Applied Entomology 9, 165–170.Google Scholar
Wright, R.J., Witkovski, J.F., Echtenkamp, G. & Georgis, R. (1993) Efficiency and persistence of Steinernema carpocapsae (Rhabditida: Steinernematidae) applied through center-pivot irrigation system against larval corn rootworms (Coleoptera: Chrysomelidae). Journal of Economic Entomology 86, 1348–1354.CrossRefGoogle Scholar