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Resolution of three cryptic agricultural pests (Ceratitis fasciventris, C. anonae, C. rosa, Diptera: Tephritidae) using cuticular hydrocarbon profiling

Published online by Cambridge University Press:  04 June 2014

L. Vaníčková ,
M. Virgilio ,
A. Tomčala ,
R. Břízová ,
S. Ekesi ,
M. Hoskovec ,
B. Kalinová ,
R. R. Do Nascimento  and
M. De Meyer
L. Vaníčková*
Affiliation:
Institute of Chemistry and Biotechnology, Federal University of Alagoas, BR 104 Norte Km 14, 57072-970 Maceió, Alagoas, Brazil Institute of Organic Chemistry and Biochemistry of the ASCR, Flemingovo nám. 2, CZ-166 10 Prague 6, Czech Republic
M. Virgilio
Affiliation:
Royal Museum for Central Africa, Leuvensesteenweg 13, B-3080 Tervuren, Belgium
A. Tomčala
Affiliation:
Institute of Organic Chemistry and Biochemistry of the ASCR, Flemingovo nám. 2, CZ-166 10 Prague 6, Czech Republic
R. Břízová
Affiliation:
Institute of Organic Chemistry and Biochemistry of the ASCR, Flemingovo nám. 2, CZ-166 10 Prague 6, Czech Republic Institute of Chemical Technology in Prague, Technická 5, CZ-166 28 Prague 6, Czech Republic
S. Ekesi
Affiliation:
International Centre of Insect Physiology and Ecology, PO Box 30772-00100 GPO, Nairobi, Kenya
M. Hoskovec
Affiliation:
Institute of Organic Chemistry and Biochemistry of the ASCR, Flemingovo nám. 2, CZ-166 10 Prague 6, Czech Republic
B. Kalinová
Affiliation:
Institute of Organic Chemistry and Biochemistry of the ASCR, Flemingovo nám. 2, CZ-166 10 Prague 6, Czech Republic
R. R. Do Nascimento
Affiliation:
Institute of Chemistry and Biotechnology, Federal University of Alagoas, BR 104 Norte Km 14, 57072-970 Maceió, Alagoas, Brazil
M. De Meyer
Affiliation:
Royal Museum for Central Africa, Leuvensesteenweg 13, B-3080 Tervuren, Belgium
*
*Author for correspondence E-mail: [email protected]
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Abstract

Discrimination of particular species within the species complexes of tephritid fruit flies is a very challenging task. In this fruit-fly family, several complexes of cryptic species have been reported, including the African cryptic species complex (FAR complex). Cuticular hydrocarbons (CHCs) appear to be an excellent tool for chemotaxonomical discrimination of these cryptic species. In the present study, CHC profiles have been used to discriminate among three important agricultural pests from the FAR complex, Ceratitis fasciventris, Ceratitis anonae and Ceratitis rosa. Hexane body surface extracts of mature males and females were analyzed by two-dimensional gas chromatography with mass spectrometric detection and differences in CHC profiles between species and sexes tested through multivariate statistics and compared with species identification by means of microsatellite markers. Quantitative as well as qualitative CHC profile differences between sexes and species are reported. The CHC profiles consisted of a mixture of linear, internally methyl-branched and mono-, di- and tri-unsaturated alkanes. Twelve compounds were pinpointed as potential chemotaxonomical markers. The present study shows that presence or absence of particular CHCs might be used in the chemical diagnosis of the FAR complex. Moreover, our results represent an important first step in the development of a useful chemotaxonomic tool for cryptic species identification of these important agricultural pests.

Keywords

cryptic species complexgenus Ceratitiscuticular hydrocarbonspolymorphic microsatellite locichemotaxonomy
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 104 , Issue 5 , October 2014 , pp. 631 - 638
Copyright
Copyright © Cambridge University Press 2014 

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