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Real-time PCR assay for distinguishing Frankliniella occidentalis and Thrips palmi Arnika Przybylska, Żaneta Fiedler, Aleksandra Obrępalska-Stęplowska

Published online by Cambridge University Press:  01 March 2017

Arnika Przybylska ,
Żaneta Fiedler ,
Patryk Frąckowiak  and
Aleksandra Obrępalska-Stęplowska
Arnika Przybylska
Affiliation:
Institute of Plant Protection – National Research Institute, 20 Władysława Węgorka St, Poznań, Poland
Żaneta Fiedler
Affiliation:
Institute of Plant Protection – National Research Institute, 20 Władysława Węgorka St, Poznań, Poland
Patryk Frąckowiak
Affiliation:
Institute of Plant Protection – National Research Institute, 20 Władysława Węgorka St, Poznań, Poland
Aleksandra Obrępalska-Stęplowska*
Affiliation:
Institute of Plant Protection – National Research Institute, 20 Władysława Węgorka St, Poznań, Poland
*
*Author for correspondence Tel.: +48 61 8649145 Fax: +48 61 8676301 E-mail: [email protected]
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Abstract

Thrips palmi and Frankliniella occidentalis (order Thysanoptera) are thrips species that represent major plant pests. They are polyphagous insects capable of adversely affecting crop production. As such, in the European Union, these thrips species should be regulated as quarantine organisms. T. palmi and F. occidentalis can cause considerable damage to susceptible plants by feeding on them and transmitting several viruses responsible for serious plant diseases. Successful pest control strategies are based on an early, fast, and reliable diagnosis, which precedes the selection of appropriate steps to limit the effects of harmful organisms. We herein describe a novel diagnostic approach that enables the sensitive and species-specific detection (and differentiation) of these pests in a duplex polymerase chain reaction assay, which was adapted for both standard and real-time quantitative assays. Our method is based on the amplification of a 5.8S-internal transcribed spacer 2 ribosomal DNA fragment that is conserved between T. palmi and F. occidentalis.

Keywords

insect detectiondiagnostic protocolrDNAquarantine pestsmolecular methodsmultiplex PCRpest control
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 108 , Issue 3 , June 2018 , pp. 413 - 420
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Copyright
Copyright © Cambridge University Press 2017 

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