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Novel use of stir bar sorptive extraction (SBSE) as a tool for isolation of oviposition site attractants for gravid Culex quinquefasciatus

Published online by Cambridge University Press:  23 March 2009

C. Carson ,
M.A. Birkett ,
J.G. Logan ,
K. Mawa ,
H.V. Pates ,
J.A. Pickett ,
R.T. Rwegoshora ,
P.K. Tungu  and
M.M. Cameron
C. Carson*
Affiliation:
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, LondonWC1E 7HT, UK
M.A. Birkett
Affiliation:
Centre for Sustainable Pest and Disease Management, Biological Chemistry Department, Rothamsted Research, Harpenden, UK
J.G. Logan
Affiliation:
Centre for Sustainable Pest and Disease Management, Biological Chemistry Department, Rothamsted Research, Harpenden, UK
K. Mawa
Affiliation:
National Institute for Medical Research, Amani Medical Research Centre, Muheza, Tanga, United Republic of Tanzania
H.V. Pates
Affiliation:
National Institute for Medical Research, Amani Medical Research Centre, Muheza, Tanga, United Republic of Tanzania
J.A. Pickett
Affiliation:
Centre for Sustainable Pest and Disease Management, Biological Chemistry Department, Rothamsted Research, Harpenden, UK
R.T. Rwegoshora
Affiliation:
National Institute for Medical Research, Amani Medical Research Centre, Muheza, Tanga, United Republic of Tanzania
P.K. Tungu
Affiliation:
National Institute for Medical Research, Amani Medical Research Centre, Muheza, Tanga, United Republic of Tanzania
M.M. Cameron
Affiliation:
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, LondonWC1E 7HT, UK
*
*Author for correspondence Fax: +44 (0)24 7652 4619 E-mail: [email protected]
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Abstract

Mosquitoes such as Culex quinquefasciatus Say (Diptera: Culicidae) are important vectors of organisms that cause disease in humans. Research into the development of effective standardized odour baits for blood-fed females (oviposition attractants), to enable entomological monitoring of vector populations, is hampered by complex protocols for extraction of physiologically active volatile chemicals from natural breeding site water samples, which have produced inconsistent results. Air entrainment and solvent extraction are technically demanding methods and are impractical for use in resource poor environments where mosquito-borne disease is most prevalent. This study reports the first use of a simple, robust extraction technique, stir bar sorptive extraction (SBSE), to extract behaviourally active small lipophilic molecules (SLMs) present in water samples collected from Cx. quinquefasciatus breeding sites in Tanzania. Extracts from a pit latrine and from a cess pool breeding site attracted more gravid Cx. quinquefasciatus in pair choice bioassays than control extracts, and coupled gas chromatography-electroantennography (GC-EAG) allowed tentative identification of 15 electrophysiologically active chemicals, including the known oviposition attractant, skatole (3-methylindole). Here, we have demonstrated, using simple pair choice bioassays in controlled laboratory conditions, that SBSE is effective for the extraction of behaviourally and electrophysiologically active semiochemicals from mosquito breeding site waters. Further research is required to confirm that SBSE is an appropriate technique for use in field surveys in the search for oviposition cues for Cx. quinquefasciatus.

Keywords

Culex quinquefasciatusovipositionSBSEsemiochemicalelectroantennography
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 100 , Issue 1 , February 2010 , pp. 1 - 7
Copyright
Copyright © 2009 Cambridge University Press

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References

Baltussen, E., Sandra, P., David, F. & Cramers, C. (1999) Stir bar sorptive extraction (SBSE), a novel extraction technique for aqueous samples: Theory and principles. Journal of Microcolumn Separations 11, 737–747.Google Scholar
Barbosa, R.M.R., Souto, A., Eiras, A.E. & Regis, L. (2007) Laboratory and field evaluation of an oviposition trap for Culex quinquefasciatus (Diptera: Culicidae). Memorias do Instituto Oswaldo Cruz 102, 523–529.CrossRefGoogle ScholarPubMed
Becker, N., Boase, C., Dahl, C., Kaiser, A., Lane, J., Petric, D. & Zgomba, M. (2003) Mosquitoes and Their Control. 518 pp. New York, Kluwer Academic/Plenum Publishers.CrossRefGoogle ScholarPubMed
Beehler, J.W., Millar, J.G. & Mulla, M.S. (1994) Field evaluation of synthetic compounds mediating oviposition in Culex mosquitos (Diptera: Culicidae). Journal of Chemical Ecology 20, 281–291.Google Scholar
Blackwell, A., Mordue, A.J., Hansson, B.S., Wadhams, L.J. & Pickett, J.A. (1993) A behavioural and electrophysiological study of oviposition cues for Culex quinquefasciatus. Physiological Entomology 18, 343–348.CrossRefGoogle Scholar
Dethier, V.G., Browne, L.B. & Smith, C.N. (1960) The designation of chemicals in terms of the responses they elicit from insects. Journal of Economic Entomology 53, 134–136.CrossRefGoogle Scholar
Du, Y.J. & Millar, J.G. (1999) Electroantennogram and oviposition bioassay responses of Culex quinquefasciatus and Culex tarsalis (Diptera: Culicidae) to chemicals in odors from Bermuda grass infusions. Journal of Medical Entomology 36, 158–166.CrossRefGoogle ScholarPubMed
Godsey, M.S. Jr, Nasci, R., Savage, H.M., Aspen, S., King, R., Powers, A.M., Burkhalter, K., Colton, L., Charnetzky, D., Lasater, S., Taylor, V. & Palmisano, C.T. (2005) West Nile virus-infected mosquitoes, Louisiana, 2002. Emerging Infectious Disease 11, 1399–1404.Google Scholar
Isoe, J., Beehler, J.W., Millar, J.G. & Mulla, M.S. (1995a) Oviposition responses of Culex tarsalis and Culex quinquefasciatus to aged Bermuda grass infusions. Journal of the American Mosquito Control Association 11, 39–44.Google ScholarPubMed
Isoe, J., Millar, J.G. & Beehler, J.W. (1995b) Bioassays for Culex (Diptera: Culicidae) mosquito oviposition attractants and stimulants. Journal of Medical Entomology 32, 475–483.Google Scholar
Kawaguchi, M., Inoue, K., Sakui, N., Ito, R., Izumi, S., Makino, T., Okanouchi, N. & Nakazawa, H. (2004) Stir bar sorptive extraction and thermal desorption-gas chromatography-mass spectrometry for the measurement of 4-nonylphenol and 4-tert-octylphenol in human biological samples. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences 799, 119–125.Google Scholar
Kirby, W. & Spence, W. (1826) An Introduction to Entomology. 607 pp. London, Longman, Rees, Orme, Brown, and Green.Google Scholar
Kramer, W.L. & Mulla, M.S. (1979) Oviposition attractants and repellents of mosquitos – Oviposition responses of Culex (Diptera: Culicidae) mosquitos to organic infusions. Environmental Entomology 8, 1111–1117.CrossRefGoogle Scholar
Laurence, B.R. & Pickett, J.A. (1985) An oviposition attractant pheromone in Culex quinquefasciatus Say (Diptera: Culicidae). Bulletin of Entomological Research 75, 283–290.CrossRefGoogle Scholar
Linstrom, P.J. & Mallard, W.G. (Eds) (2007) NIST Chemistry WebBook, NIST Standard Reference Database Number 69, National Institute of Standards and Technology, Gaithersburg MD, http://webbook.nist.gov (accessed 20 October 2007).Google Scholar
Logan, J.G., Birkett, M.A., Clark, S.J., Powers, S., Seal, N.J., Wadhams, L.J., Mordue Luntz, A.J. & Pickett, J.A. (2008) Identification of human-derived volatile chemicals that interfere with attraction of Aedes aegypti mosquitoes. Journal of Chemical Ecology 34, 308–322.Google Scholar
McCall, P.J. & Eaton, G. (2001) Olfactory memory in the mosquito Culex quinquefasciatus. Medical and Veterinary Entomology 15, 197–203.Google Scholar
Millar, J.G., Chaney, J.D. & Mulla, M.S. (1992) Identification of oviposition attractants for Culex quinquefasciatus from fermented Bermuda grass Infusions. Journal of the American Mosquito Control Association 8, 11–17.Google Scholar
Millar, J.G., Chaney, J.D., Beehler, J.W. & Mulla, M.S. (1994) Interaction of the Culex quinquefasciatus egg raft pheromone with a natural chemical associated with oviposition sites. Journal of the American Mosquito Control Association 10, 374–379.Google ScholarPubMed
Mordue, A.J., Blackwell, A., Hansson, B.S., Wadhams, L.J. & Pickett, J.A. (1992) Behavioral and electrophysiological evaluation of oviposition attractants for Culex quinquefasciatus Say (Diptera: Culicidae). Experientia 48, 1109–1111.Google Scholar
Muturi, E.J., Mwangangi, J., Shililu, J., Muriu, S., Jacob, B., Mbogo, C.M., John, G. & Novak, R. (2007) Evaluation of four sampling techniques for surveillance of Culex quinquefasciatus (Diptera: Culicidae) and other mosquitoes in African rice agroecosystems. Journal of Medical Entomology 44, 503–508.CrossRefGoogle ScholarPubMed
Olagbemiro, T.O., Birkett, M.A., Mordue, A.J. & Pickett, J.A. (2004) Laboratory and field responses of the mosquito, Culex quinquefasciatus, to plant-derived Culex spp. oviposition pheromone and the oviposition cue skatole. Journal of Chemical Ecology 30, 965–976.Google Scholar
Pile, M.M., Simmonds, M.S.J. & Blaney, W.M. (1991) Odor-mediated upwind flight of Culex quinquefasciatus mosquitos elicited by a synthetic attractant. Physiological Entomology 16, 77–85.Google Scholar
Qiu, Y.T. (2005) Sensory and behavioural responses of the malaria mosquito Anopheles gambiae to human odours. PhD thesis, Wageningen University, The Netherlands.Google Scholar
Reisen, W.K. & Meyer, R.P. (1990) Attractiveness of selected oviposition substrates for gravid Culex tarsalis and Culex quinquefasciatus in California. Journal of the American Mosquito Control Association 6, 244–250.Google ScholarPubMed
Reiter, P. (1983) A portable, battery-powered trap for collecting gravid Culex Mosquitos. Mosquito News 43, 496–498.Google Scholar
Reiter, P. (1987) A revised version of the CDC gravid mosquito trap. Journal of the American Mosquito Control Association 3, 325–327.Google ScholarPubMed
Ritchie, S.A. (1984) Hay infusion and isopropyl alcohol-baited CDC light trap – a simple, effective trap for gravid Culex mosquitos. Mosquito News 44, 404–407.Google Scholar
Rwegoshora, R.T., Pedersen, E.M., Mukoko, D.A., Meyrowitsch, D.W., Masese, N., Malecela-Lazaro, M.N., Ouma, J.H., Michael, E. & Simonsen, P.E. (2005) Bancroftian filariasis: Patterns of vector abundance and transmission in two East African communities with different levels of endemicity. Annals of Tropical Medicine and Parasitology 99, 253–265.CrossRefGoogle ScholarPubMed
Sant'ana, A.L., Roque, R.A. & Eiras, A.E. (2006) Characteristics of grass infusions as oviposition attractants to Aedes (Stegomyia) (Diptera: Culicidae). Journal of Medical Entomology 43, 214–220.Google Scholar
Serodio, P. & Nogueira, J.M.F. (2005) Development of a stir-bar-sorptive extraction-liquid desorption-large-volume injection capillary gas chromatographic-mass spectrometric method for pyrethroid pesticides in water samples. Analytical and Bioanalytical Chemistry 382, 1141–1151.Google Scholar
Subra, R. (1981) Biology and Control of Culex pipiens quinquefasciatus Say, 1823 (Diptera, Culicidae) with Special Reference to Africa. Insect Science and Its Application 1, 319–338.Google Scholar
Sucharit, S., Surathin, K. & Shrestha, S.R. (1989) Vectors of Japanese encephalitis virus (JEV): species complexes of the vectors. The Southeast Asian journal of Tropical Medicine and Public Health 20, 611–621.Google Scholar
Surgeoner, G.A. & Helson, B.V. (1978) Oviposition trap for Arbovirus surveillance in Culex sp mosquitos (Diptera: Culicidae). Canadian Entomologist 110, 1049–1052.Google Scholar