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Morphology and preliminary enzyme characterization of the salivary glands from the predatory bug Podisus nigrispinus (Heteroptera: Pentatomidae)

Published online by Cambridge University Press:  09 March 2007

J.A. Oliveira ,
M.G.A. Oliveira ,
R.N.C. Guedes  and
M.J. Soares
J.A. Oliveira
Affiliation:
Departamento de Biologia Animal, Universidade Federal de Viçosa, Viçosa, MG 36571-000, Brazil
M.G.A. Oliveira*
Affiliation:
Departamento de Bioquímica e Biologia Molecular, Instituto de Biotecnologia Aplicada a Agropecuária (BIOAGRO), Universidade Federal de Viçosa, Viçosa, MG 36571-000, Brazil
R.N.C. Guedes
Affiliation:
Departamento de Biologia Animal, Universidade Federal de Viçosa, Viçosa, MG 36571-000, Brazil
M.J. Soares
Affiliation:
Departamento de Ultraestrutura e Biologia Celular, Fundação Instituto Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, RJ 21040-900, Brazil
*
*Fax (+55)(31)3899 4012 Email: [email protected]
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Abstract

Podisus nigrispinus (Dallas) is a common predator in agricultural and natural systems in Neotropical America. Its feeding strategy involves extra-oral digestion and to better understand this process its salivary glands were extracted and subjected to morphological and preliminary enzyme characterization. The salivary glands of P. nigrispinus are formed by a pair of main and accessory gland complexes. The main salivary glands are further divided into an anterior and a posterior lobe. The compartmentalization of the salivary gland complex is likely to be important for the production, activation and release of the digestive enzymes used in the extra-oral digestion of prey items. Proteases and lipase, important digestive enzymes involved in zoophagy, were detected in the salivary glands of P. nigrispinus. The prevailing trypsin-like protease activity was characterized by using the serine-protease substrate N-α-benzoyl-L-Arg-p-nitroanilidine (L-BApNA) and the trypsin inhibitors tosyl-L-lysine chloromethyl ketone (TLCK) and benzamidine. The KM value obtained for trypsin-like activity was 1.57 mm and the different peaks of optimum pH and temperature activity suggest the presence of multiple forms of this enzyme in P. nigrispinus. Detection of amylase activity in the salivary glands of this predator suggests its ability to digest starch and obtain nutrients from plants, which may have adaptative value under prey scarcity.

Keywords

amylaselipasetrypsin-like proteaseHeteropteraPentatomidaeAsopinaeextra-oral digestionspined soldier bug
Type
Review Article
Information
Bulletin of Entomological Research , Volume 96 , Issue 3 , June 2006 , pp. 251 - 258
Copyright
Copyright © Cambridge University Press 2006

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