Hostname: page-component-586b7cd67f-l7hp2 Total loading time: 0 Render date: 2024-11-23T22:31:20.388Z Has data issue: false hasContentIssue false
  • English
  • Français

Molecular differences in the mitochondrial cytochrome oxidase I (mtCOI) gene and development of a species-specific marker for onion thrips, Thrips tabaci Lindeman, and melon thrips, T. palmi Karny (Thysanoptera: Thripidae), vectors of tospoviruses (Bunyaviridae)

Published online by Cambridge University Press:  04 October 2007

R. Asokan ,
N.K. Krishna Kumar ,
Vikas Kumar  and
H.R. Ranganath
R. Asokan*
Affiliation:
Division of Biotechnology, Indian Institute of Horticultural Research (IIHR), Hessaraghatta Lake (PO), Bangalore, 560 089, India
N.K. Krishna Kumar
Affiliation:
Division of Entomology & Nematology, Indian Institute of Horticultural Research (IIHR), Hessaraghatta Lake (PO), Bangalore, 560 089, India
Vikas Kumar
Affiliation:
Division of Entomology & Nematology, Indian Institute of Horticultural Research (IIHR), Hessaraghatta Lake (PO), Bangalore, 560 089, India
H.R. Ranganath
Affiliation:
Division of Entomology & Nematology, Indian Institute of Horticultural Research (IIHR), Hessaraghatta Lake (PO), Bangalore, 560 089, India
*
*Fax: 91 80 28466291 E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

A quick and developmental-stage non-limiting method of the identification of vectors of tospoviruses, such as Thrips tabaci and T. palmi, is important in the study of vector transmission, insecticide resistance, biological control, etc. Morphological identification of these thrips vectors is often a stumbling block in the absence of a specialist and limited by polymorphism, sex, stage of development, etc. Molecular identification, on the other hand, is not hampered by the above factors and can easily be followed by a non-specialist with a little training. The mitochondrial cytochrome oxidase I (mtCOI) exhibits reliable inter-species variations as compared to the other markers. In this communication, we present the differences in the mtCOI partial sequence of morphologically identified specimens of T. tabaci and T. palmi collected from onion and watermelon, respectively. Species-specific markers, identified in this study, could successfully determine T. tabaci and T. palmi, which corroborated the morphological identification. Phylogenetic analyses showed that both T. tabaci and T. palmi formed different clades as compared to the other NCBI accessions. The implication of these variations in vector efficiency has to be investigated further. The result of this investigation is useful in the quick identification of T. tabaci and T. palmi, a critical factor in understanding the epidemiology of the tospoviruses, their management and also in quarantine.

Keywords

Thrips tabaciT. palmimtCOIspecies-specific marker
Type
Research Article
Information
Bulletin of Entomological Research , Volume 97 , Issue 5 , October 2007 , pp. 461 - 470
Copyright
Copyright © Cambridge University Press 2007

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Barr, N.B., Gui, L. & McPheron, B.A. (2005) Molecular systematics of nuclear gene period in genus Anastrapha (Tephritidae). Annals of Entomological Society of America 98, 173–180.CrossRefGoogle Scholar
Bayar, K., Torjek, O., Kiss, E., Gyulai, G. & Heszky, L. (2001) Genetic variation within and among populations of Aeolothrips intermedius. pp. 369–372. in Proceedings of the 7th International Symposium on Thysanoptera. 1–8 July 2001, Reggio Calabria, Italy.Google Scholar
Bayar, K., Torjek, O., Kiss, E., Gyulai, G. & Heszky, L. (2002) Intra and inter specific molecular polymorphism of thrips species. Acta Biologica Hungarica 53, 317–324.CrossRefGoogle Scholar
Bhatti, J.S. (1980) Species of the genus Thrips from India. Systematic Entomology 5, 109–166.CrossRefGoogle Scholar
Birnboim, H.C. & Dolly, J.A. (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7, 1513–1523.CrossRefGoogle ScholarPubMed
Brunner, P.C., Chatzivassilious, E.K., Katis, N.I & Frey, J.E. (2004) Host-associated genetic differentiation in Thrips tabaci (Insecta: Thysanoptera), as determined from mtDNA sequence data. Heredity 93, 364–370.CrossRefGoogle ScholarPubMed
Brunner, P.C., Flemming, C. & Frey, J.E. (2002) A molecular identification key for economically important thrips species (Thysanoptera: Thripidae) using direct sequencing and a PCR-RFLP based approach. Agriculture and Forest Entomology 4, 127–136.CrossRefGoogle Scholar
Frey, J.E. & Frey, B. (2004) Origin of intra-individual variation in PCR-amplified mitochondrial cytochrome oxidase I of T. tabaci (Thysanoptera: Thripidae): mitochodrial heteroplasmy or nuclear integration? Heriditas 140, 92–98.CrossRefGoogle ScholarPubMed
Gyulai, G.K., Bayar, K., Torjek, O., Kiss, E., Kiss, J., Szabo, Z. & Heszky, L. (2001) Molecular polymorphism among populations of Frankliniella intonsa. pp. 373–375 in Proceedings of the 7th International Symposium on Thysanoptera. 1–8 July 2001, Reggio Calabria, Italy.Google Scholar
Kim, K.S. & Sappington, T.W. (2005) Genetic structuring of Western corn root worm (Coleoptera: Chrysomelidae) populations in the United States based on microsatellite loci analysis. Environmental Entomology 34, 494–503.CrossRefGoogle Scholar
Kjer, K.M. (2004) Aligned 18S and insect phylogeny. Systematic Biology 53, 506–514.CrossRefGoogle ScholarPubMed
Kox, L.F.F., Vanden Beld, H.E., Zijlstra, C. & Vierbergen, G. (2005) Real-time PCR assay for the identification of Thrips palmi. EPPO Bulletin 35, 141–148.CrossRefGoogle Scholar
Moritz, G., Delker, C., Paulsen, M., Mound, L.A. & Burgermeister, W. (2000) Modern methods for identification of Thysanoptera. EPPO Bulletin 30, 591–593.CrossRefGoogle Scholar
Moritz, G., Paulsen, M., Delker, C., Picl, S. & Kumm, S. (2001) Identification of thrips suing ITS-RFLP analysis. pp. 365–367 in Proceedings of the 7th International Symposium on Thysanoptera. 1–8 July 2001, Reggio Calabria, Italy.Google Scholar
Mound, L.A. (2005) Thysanoptera: Diversity and interactions. Annual Review of Entomology 50, 247–269.CrossRefGoogle ScholarPubMed
Murai, T. & Toda, S. (2001) Variations of Thrips tabaci in color and size. pp. 377–378. in Proceedings of the 7th International Symposium on Thysanoptera. 1–8 July 2001, Reggio Calabria, Italy.Google Scholar
Page, R.D.M. (1996) TREEVIEW: An application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences 12, 357–358.Google ScholarPubMed
Ravi, K.S., Kitkaru, A.S. & Winter, S. (2006) Iris yellow spot virus in onions: a tospovirus record from India. Plant Pathology 55, 288.CrossRefGoogle Scholar
Rokas, A., Nylander, J.A.A., Ronquist, F. & Stone, G.N. (2002) A maximum likelihood analysis of eight phylogenetic markers in gallwasps (Hymenoptera: Cynipidae): Implications for insect phylogenetic studies. Molecular Phylogenetics and Evolution 22, 206–219.CrossRefGoogle ScholarPubMed
Savolainen, V., Cowan, R.S., Vogler, A.P., Roderick, G.K. & Lane, R. (2005) Towards writing the encyclopaedia of life: an introduction to DNA barcoding. Philosophical Transactions, Royal Society, Series B 360, 1805–1811.CrossRefGoogle Scholar
Singh, S.J. & Krishna Reddy, M. (1996) Watermelon bud necrosis: A new tospovirus disease. Acta Horticulturae 431, 68–77.CrossRefGoogle Scholar
Toda, S. & Komazaki, S. (2002) Identification of thrips species (Thysanoptera: Thripidae) on Japanese fruit trees by polymerase chain reaction and restriction fragment length polymorphism of the ribosomal ITS2 region. Bulletin of Entomological Research 92, 359–363.CrossRefGoogle ScholarPubMed
Ullman, D.E., Meideros, R., Campbell, L.R., Whitfield, A.E., Sherwood, J.L. & German, T.L. (2002) Thrips as vectors of tospoviruses. Advances in Botanical Research 36, 113–140.CrossRefGoogle Scholar
Whitfield, A.E., Ullman, D.E. & German, T.L. (2005) Tospovirus-Thrips interactions. Annual Review of Phytopathology 43, 451–489.CrossRefGoogle ScholarPubMed