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A molecular diagnostic for endosulfan insecticide resistance in the coffee berry borer Hypothenemus hampei (Coleoptera: Scolytidae)

Published online by Cambridge University Press:  10 July 2009

R.H. ffrench-Constant ,
J.C. Steichen  and
L.O. Brun
R.H. ffrench-Constant*
Affiliation:
Department of Entomology, University of Wisconsin-Madison, Madison, USA
J.C. Steichen
Affiliation:
Department of Entomology, University of Wisconsin-Madison, Madison, USA
L.O. Brun
Affiliation:
Institut Français de Recherche Scientifique pour le Développement en Cooperation (ORSTROM) Nouméa, New Caledonia.
*
Dr R H ffrench-Constant, Department of Ento mology, 237 Russell Laboratories, 1630 Linden Drive, University of Wisconsin-Madison, Madison, WI 53706, USA.
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Abstract

The coffee berry borer Hypothenemus hampei (Ferrari) has recently evolved high levels of resistance to endosulfan and other cyclodiene-type insecticides in New Caledonia. During population outbreaks this has contributed to levels of infestation of coffee berries reaching up to 90%. Using degenerate primers in the polymerase chain reaction (PCR) we have amplified a section of the cyclodiene resistance gene Rdl from H. hampei. This gene codes for a γ-aminobutyric acid (GABA) gated chloride ion channel. Here we report that resistant strains of H. hampei carry exactly the same single amino acid replacement (alanine to serine) as that found in resistant Drosophila melanogaster (Meigen) (Diptera: Drosophilidae). A molecular diagnostic based upon PCR-mediated amplification of specific alleles (PASA) is described. This technique is capable of detecting resistance or susceptibility in adults, larvae or eggs but not in susceptible females carrying resistant sperm. Its potential use in field monitoring is discussed.

Type
Original Articles
Information
Bulletin of Entomological Research , Volume 84 , Issue 1 , March 1994 , pp. 11 - 15
Copyright
Copyright © Cambridge University Press 1994

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