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Microsatellites and 16S sequences corroborate phenotypic evidence of trans-Andean variation in the parasitoid Microctonus hyperodae (Hymenoptera: Braconidae)

Published online by Cambridge University Press:  09 March 2007

L.M. Winder ,
C.B. Phillips ,
C. Lenney-Williams ,
R.P. Cane ,
K. Paterson ,
C.J. Vink  and
S.L. Goldson
L.M. Winder*
Affiliation:
AgResearch Limited, Biocontrol and Biosecurity Group, PO Box 60, Gerald Street, Lincoln, Canterbury, New Zealand
C.B. Phillips
Affiliation:
AgResearch Limited, Biocontrol and Biosecurity Group, PO Box 60, Gerald Street, Lincoln, Canterbury, New Zealand
C. Lenney-Williams
Affiliation:
AgResearch Limited, Biocontrol and Biosecurity Group, PO Box 60, Gerald Street, Lincoln, Canterbury, New Zealand
R.P. Cane
Affiliation:
AgResearch Limited, Biocontrol and Biosecurity Group, PO Box 60, Gerald Street, Lincoln, Canterbury, New Zealand
K. Paterson
Affiliation:
AgResearch Molecular Biology Unit, University of Otago, PO Box 56, Dunedin, New Zealand
C.J. Vink
Affiliation:
AgResearch Limited, Biocontrol and Biosecurity Group, PO Box 60, Gerald Street, Lincoln, Canterbury, New Zealand
S.L. Goldson
Affiliation:
AgResearch Limited, Biocontrol and Biosecurity Group, PO Box 60, Gerald Street, Lincoln, Canterbury, New Zealand
*
*Fax: +64 3 325 9946 E-mail: [email protected]
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Abstract

Eight South American geographical populations of the parasitoid Microctonus hyperodae Loan were collected in South America (Argentina, Brazil, Chile and Uruguay) and released in New Zealand for biological control of the weevil Listronotus bonariensis (Kuschel), a pest of pasture grasses and cereals. DNA sequencing (16S, COI, 28S, ITS1, β-tubulin), RAPD, AFLP, microsatellite, SSCP and RFLP analyses were used to seek markers for discriminating between the South American populations. All of the South American populations were more homogeneous than expected. However, variation in microsatellites and 16S gene sequences corroborated morphological, allozyme and other phenotypic evidence of trans-Andes variation between the populations. The Chilean populations were the most genetically variable, while the variation present on the eastern side of the Andes mountains was a subset of that observed in Chile.

Keywords

Microctonus hyperodaeDNA sequence16S genemicrosatellitephylogeny
Type
Research Article
Information
Bulletin of Entomological Research , Volume 95 , Issue 4 , July 2005 , pp. 289 - 298
Copyright
Copyright © Cambridge University Press 2005

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