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Macrogeographic population structure of the tsetse fly, Glossina pallidipes (Diptera: Glossinidae)

Published online by Cambridge University Press:  09 March 2007

J.O. Ouma ,
J.G. Marquez  and
E.S. Krafsur
J.O. Ouma
Affiliation:
Department of Entomology, Iowa State University, Ames, Iowa 50011, USA
J.G. Marquez
Affiliation:
Department of Entomology, Iowa State University, Ames, Iowa 50011, USA
E.S. Krafsur*
Affiliation:
Department of Entomology, Iowa State University, Ames, Iowa 50011, USA
*
*Fax: +515 294 5957 E-mail: [email protected]
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Abstract

Tsetse flies are confined to sub-Saharan Africa where they occupy discontinuous habitats. In anticipation of area-wide control programmes, estimates of gene flow among tsetse populations are necessary. Genetic diversities were partitioned at eight microsatellite loci and five mitochondrial loci in 21 Glossina pallidipes Austin populations. At microsatellite loci, Nei's unbiased gene diversity averaged over loci was 0.659 and the total number of alleles was 214, only four of which were shared among all populations. The mean number of alleles per locus was 26.8. Random mating was observed within but not among populations(fixation index FST = 0.180) and 81% of the genetic variance was within populations. Thirty-nine mitochondrial variants were detected. Mitochondrial diversities in populations varied from 0 to 0.85 and averaged 0.42, and FST=0.51. High levels of genetic differentiation were characteristic, extending even to subpopulations separated by tens and hundreds of kilometres, and indicating low rates of gene flow.

Keywords

Breeding structuregene flowmicrosatellitessingle strand conformational polymorphismtsetseDipteraGlossina pallidipes
Type
Research Article
Information
Bulletin of Entomological Research , Volume 95 , Issue 5 , October 2005 , pp. 437 - 447
Copyright
Copyright © Cambridge University Press 2005

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