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Long-term suitability of an alternative host for rearing the sugarcane stalk borer parasitoid Tetrastichus howardi

Published online by Cambridge University Press:  17 April 2024

Natalia Carolina Bermúdez Open the ORCID record for Natalia Carolina Bermúdez [Opens in a new window] ,
Nataly de la Pava Open the ORCID record for Nataly de la Pava [Opens in a new window] ,
Juan Sebastián Dueñas Cáceres Open the ORCID record for Juan Sebastián Dueñas Cáceres [Opens in a new window] ,
Christian Sherley Araújo da Silva-Torres Open the ORCID record for Christian Sherley Araújo da Silva-Torres [Opens in a new window]  and
Jorge Braz Torres Open the ORCID record for Jorge Braz Torres [Opens in a new window]
Natalia Carolina Bermúdez
Affiliation:
Departamento de Agronomia-Entomologia, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros, S/N, Dois Irmãos, 52171-900 Recife, PE, Brazil
Nataly de la Pava
Affiliation:
Facultad de Ingeniería Programa de Ingeniería Agronómica, Universidad del Magdalena, Cra 32 n 22 08, Laboratorio de Entomología, Santa Marta, Colombia
Juan Sebastián Dueñas Cáceres
Affiliation:
Departamento de Agronomia-Entomologia, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros, S/N, Dois Irmãos, 52171-900 Recife, PE, Brazil
Christian Sherley Araújo da Silva-Torres
Affiliation:
Departamento de Agronomia-Entomologia, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros, S/N, Dois Irmãos, 52171-900 Recife, PE, Brazil
Jorge Braz Torres*
Affiliation:
Departamento de Agronomia-Entomologia, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros, S/N, Dois Irmãos, 52171-900 Recife, PE, Brazil
*
Corresponding author: Jorge Braz Torres; Email: [email protected]
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Abstract

The continuous utilisation of an alternative host may influence parasitoid performance across successive generations due to conditioning in natal hosts. Tetrastichus howardi (Olliff) has successfully been reared using Tenebrio molitor L. pupae as a feasible alternative host. However, the extended rearing of T. howardi on this alternative host may impact the biological features of the parasitoids. Parasitoids were reared using T. molitor pupae for 30 consecutive generations. Quality criteria were assessed during the generations F5, F15, and F30, offering pupae of the target pest, Diatraea saccharalis (Fabr.), and compared with the F0 generation (parasitoids reared in D. saccharalis pupae). Criteria included assessments of parasitism performance, host selection, and wing form variation in the parasitoid wasps. Additionally, we examined the fecundity of T. howardi females that emerged from both hosts, considering their age, egg loading before and after one oviposition, as well as parasitism of sugarcane stalk borer pupae. Rearing T. howardi using pupae of T. molitor did not affect its biological traits or preference for the target pest for 30 generations. After parasitism, the parasitoid left the host pupa inside the stalk, and one oviposition was enough to kill D. saccharalis pupae and obtain viable parasitoid progeny. Female sexual maturation and egg loading occurred 72 and 96 h after parasitoid emergence. Egg-loading recovery after parasitism did not happen within 24 h. T. howardi can be reared for up to 30 generations using alternative hosts without compromising its parasitism performance or egg loading.

Keywords

applied biological controlCrambidaeDiatraea saccharalisgeometric morphometricsquality control
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 114 , Issue 3 , June 2024 , pp. 347 - 358
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Copyright © Universidade Federal Rural de Pernambuco, 2024. Published by Cambridge University Press

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