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Laboratory apparatus and techniques for the rearing and insecticidal treatment of whitefly Bemisia tabaci (Homoptera: Aleyrodidae) under simulated field conditions

Published online by Cambridge University Press:  10 July 2009

Mark Rowland
Affiliation:
AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Herts, UK.
Barry Pye
Affiliation:
AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Herts, UK.
Mary Stribley
Affiliation:
AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Herts, UK.
Barbara Hackett
Affiliation:
AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Herts, UK.
Ian Denholm
Affiliation:
AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Herts, UK.
Roman M. Sawicki
Affiliation:
AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Herts, UK.

Abstract

Laboratory apparatus and techniques are described for the rearing and insecticidal treatment of whitefly, Bemisia tabaci Gennadius, under simulated field conditions. Insects were reared on cotton plants inside large population cages and treated from an overhead sprayer. The effects of these treatments were assessed accurately, without interfering with insects or plants, by monitoring adult numbers with an endoscope over one or more generations. Examples of single-generation and multiple-generation tests with cypermethrin are described. The apparatus is suitable for testing strategies for delaying the selection of resistance (e.g. using insecticides applied singly, alternately, or in mixture, at various application rates and frequencies), for controlling populations already resistant to insecticides, and for integrated pest management using chemical and biological control agents together.

Type
Original Articles
Copyright
Copyright © Cambridge University Press 1990

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