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Insecticidal effects of extracts of Humulus lupulus (hops) L. cones and its principal component, xanthohumol

Published online by Cambridge University Press:  23 March 2017

T. Aydin ,
N. Bayrak ,
E. Baran  and
A. Cakir
T. Aydin*
Affiliation:
Agri İbrahim Cecen University, Faculty of Pharmacy, Department of Pharmacognosy, 04100 Agri, TR, Turkey
N. Bayrak
Affiliation:
Bozok University, Faculty of Agriculture, Department of Plant Protection, 66900 Yozgat, TR, Turkey
E. Baran
Affiliation:
Kilis 7 Aralık University, Faculty of Sciences & Arts, Department of Chemistry, 79000 Kilis, TR, Turkey
A. Cakir
Affiliation:
Kilis 7 Aralık University, Faculty of Sciences & Arts, Department of Chemistry, 79000 Kilis, TR, Turkey
*
*Author for correspondence Phone: +90 (472) 2159863 Fax: +90 (472) 2151182 E-mail: [email protected]
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Abstract

Insecticidal effects of the dichloromethane, ethyl acetate, acetone, ethanol and methanol extracts of Humulus lupulus (hops) L. cones and its principal components, xanthohumol was investigated on five stored pests, Sitophilus granarius (L.), Sitophilus oryzae (L.), Acanthoscelides obtectus (Say.), Tribolium castaneum (Herbst) and Lasioderma serricorne (F.). The mortality of adults of the insects treated with 2, 5, 5, 10 and 20 mg ml̠−1 concentrations of the extracts and xanthuhumol was counted after 24, 48, 72, 96 and 120 h. In order to determine the toxic effects of the substances tested against all tested insects, durations for 50% mortality of the adults, and LD50 values were also determined in the first 48 h by probit analysis. Our results also showed that xanthohumol was more toxic against the pests in comparison with the extracts applications. LD50 values for xanthohumol were found to be low dose as compared with the extracts. Xanthohumol was more toxic against S. granarius (L.) with 6.8 µg of LD50 value. Among the extracts, methanol extract was less effective than other extracts against all tested insects. The ethyl acetate extract of H. lupulus cones was the most effective extract against the tested pests. The quantitative amounts of xanthohumol in the extracts were determined using a high-performance liquid chromatography. The quantitative data indicated that amount of xanthohumol in the extracts increased with increase of polarity of the solvents used from methanol to dichloromethane. The methanol extract contained the high amount of xanthohumol with 5.74 g/100 g extract (0.46 g/100 g plant sample).

Keywords

Humulus lupulusinsecticidal effectxanthohumolstored pest
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 107 , Issue 4 , August 2017 , pp. 543 - 549
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Copyright
Copyright © Cambridge University Press 2017 

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