Hostname: page-component-586b7cd67f-tf8b9 Total loading time: 0 Render date: 2024-11-27T23:00:25.381Z Has data issue: false hasContentIssue false
  • English
  • Français

In vitro rearing of Glossina palpalis palpalis (Robineau-Desvoidy) (Diptera: Glossinidae) on guinea-pig blood

Published online by Cambridge University Press:  10 July 2009

J. R. Deloach  and
M. Taher
J. R. Deloach
Affiliation:
International Atomic Energy Agency, Joint FAO/IAEA Laboratory, Vienna, Austria
M. Taher
Affiliation:
International Atomic Energy Agency, Joint FAO/IAEA Laboratory, Vienna, Austria
Get access Rights & Permissions [Opens in a new window]

Abstract

The productivity of Glossina palpalis palpalis (R.-D.) reared in vitro via silicone rubber membranes on fresh guinea-pig blood equalled the productivity of flies fed in vivo on guinea-pigs. Over 50 days, the number of puparia per initial female was 3·0 in both cases. Puparial weights were greater than 31 mg throughout the four reproductive cycles. While fresh blood was an excellent diet for G. p. palpalis, freeze-dried guinea-pig blood was unsuitable; survival, reproduction and puparial weights were all low for flies fed on freeze-dried blood. The evidence lends support to the theory that there is no inherent nutritional deficiency caused by feeding on blood through a membrane.

Type
Original Articles
Information
Bulletin of Entomological Research , Volume 72 , Issue 4 , December 1982 , pp. 663 - 667
Copyright
Copyright © Cambridge University Press 1982

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bauer, B. & Aigner, H. (1978). In vitro maintenance of Glossina palpalis palpalis (Robineau-Desvoidy) (Diptera: Glossinidae).—Bull. ent. Res. 68, 393–400.CrossRefGoogle Scholar
Bauer, B. & Wetzel, H. (1976). A new membrane for feeding Glossina morsitans Westw. (Diptera, Glossinidae).—Bull. ent. Res. 65, 563–565.CrossRefGoogle Scholar
Evens, F., Van Der Vloedt, A. & Haeseleer, F. D. (1970). The quantitative and qualitative composition of increasing population of Glossina palpalis and Glossina quanzensis, at different times, during the process of growth.—pp. 103–115in de Azevedo, J. F. (Ed.). Tsetse fly breeding under laboratory conditions and its practical application. 1st international symposium 22nd and 23rd April 1969.—524 pp. Lisbon, Junta Invest. Ultramar.Google Scholar
Langley, P. A. (1972). Further experiments on rearing of tsetse flies in the absence of a living host.—Trans. R. Soc. trop. Med. Hyg. 66, 310.CrossRefGoogle ScholarPubMed
Langley, P. A. & Maly, H. (1969). Membrane feeding technique for tsetse flies (Glossina spp.).—Nature, Lond. 221, 855–856.CrossRefGoogle ScholarPubMed
Mews, A. R., Baumgartner, H., Luger, D. & Offori, E. D. (1976). Colonisation of Glossina morsitans morsitans Westw. (Diptera, Glossinidae) in the laboratory using in vitro feeding techniques.—Bull. ent. Res. 65, 631–642.CrossRefGoogle Scholar
Mews, A. R., Langley, P. A., Pimley, R. W. & Flood, M. E. T. (1977). Large-scale rearing of tsetse flies (Glossina spp.) in the absence of a living host.—Bull. ent. Res. 67, 119–128.CrossRefGoogle Scholar
Van Der Vloedt, A. (1975). L'élevage au laboratoire de Glossina palpalis palpalis et de Glossina fuscipes quanzensis.—pp. 61–62in Institut d'Élevage et de Médecine Vétérinaire des Pays Tropicaux. Control programs for trypanosomes and their vectors. Proceedings of the colloquium, Paris, 12–15 March 1974.—387 pp. Maisons-Alfort, IEMVT.Google Scholar
Wetzel, H. (1979 a). Zuchtung von Tsetsefliegen mit Membranefütterung.—GSF-Bericht BT 571 (Dissertation), Munich, Gesellschaft für Strahlen- und Umweltforschung.Google Scholar
Wetzel, H. (1979 b). Artificial membrane for in vitro feeding of piercing-sucking arthropods.—Entomologia exp. appl. 25, 117–119.CrossRefGoogle Scholar
Wetzel, H. (1980). The use of freeze-dried blood in the membrane feeding of tsetse flies (Glossina p. palpalis, Diptera: Glossinidae).—Tropenmed. & Parasitol. 31, 259–274.Google ScholarPubMed
Wetzel, H. & Luger, D. (1978). The use of deep frozen, stored bovine blood for in vitro feeding of tsetse flies.—Z. ParasitKde 57, 163–168.CrossRefGoogle ScholarPubMed