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In vitro maintenance of Glossina palpalis palpalis (Robineau-Desvoidy) (Diptera: Glossinidae)

Published online by Cambridge University Press:  10 July 2009

B. Bauer  and
H. Aigner
B. Bauer
Affiliation:
International Atomic Energy Agency, Vienna, Austria
H. Aigner
Affiliation:
International Atomic Energy Agency, Vienna, Austria
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Abstract

A silicone membrane was developed to meet the feeding requirements of Glossina palpalis palpalis (R.-D.). Starting with a total of 1050 females, a self-sustaining colony fed on defibrinated pig blood was established. The colony performance in terms of mean daily mortality, mean pupal weight and fecundity was evaluated. With the exception of June 1976, the mean daily mortality ranged between 0·40% and 1·20%. During June 1976, bacterial infections caused a sudden increase in mortality. Escherichia coli and Staphylococcus spp. were predominant in the gut of dead and diseased flies. Pure cultures of either organism caused the same symptoms in teneral flies within one week after uptake of an infected blood-meal. The weight of the offspring significantly decreased with increasing female age. Starting with a mean pupal weight of 30 mg there was a decrease of 4–6 mg over a period of 90 days. The innate capacity for increase in numbers (r-) was calculated based on data from 340 females. The value of rm = 0·0095 would result in a 32-fold increase of the colony in one year.

Type
Original Articles
Information
Bulletin of Entomological Research , Volume 68 , Issue 3 , September 1978 , pp. 393 - 400
Copyright
Copyright © Cambridge University Press 1978

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