Hostname: page-component-cd9895bd7-jn8rn Total loading time: 0 Render date: 2024-12-26T00:23:33.488Z Has data issue: false hasContentIssue false
  • English
  • Français

Identification of the 2-tridecanone responsive region in the promoter of cytochrome P450 CYP6B6 of the cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae)

Published online by Cambridge University Press:  02 October 2014

F. Li ,
X.N. Liu ,
Y. Zhu ,
J. Ma ,
N. Liu  and
J.H. Yang
F. Li
Affiliation:
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China
X.N. Liu*
Affiliation:
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China
Y. Zhu
Affiliation:
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China
J. Ma
Affiliation:
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China
N. Liu
Affiliation:
National Cotton Engineering &Technology Research Center, Urumqi 830091, China
J.H. Yang
Affiliation:
Department of Pediatrics, Texas Children's Cancer Center, Dan L. Duncan Cancer Center, Baylor College of Medicine, Houston, TX 77030, USA
*
*Author for correspondence Phone: 13579817922 E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Eukaryote transcription is controlled by regulatory DNA sequences and transcription factors, so transcriptional control of gene plays a pivotal role in gene expression. In this study, we identified the region of the CYP6B6 gene promoter of Helicoverpa armigera which responds to the plant secondary toxicant 2-tridecanone. Transient transfection assay results from five of stepwise deletion fragments linked to the luciferase reporter gene revealed that the promoter activity of each CYP6B6 fragment was significantly higher than that of their basal activity after the Sf9 cells were treated with 2-tridecanone. Among all, the fragment spanning −373 to +405 bp of the CYP6B6 promoter showed an obviously 2-tridecanone inducibility (P<0.0001), which might have the 2-tridecanone responsive element based on promoter activity. Electrophoretic mobility shift assays revealed that the nuclear protein extracted from midgut of the 6th instar larva of H. armigera, reared on 10 mg 2-tridecanone per gram artificial diet for 48 h, could specifically bind to the active region from −373 to 21 bp of the CYP6B6 promoter. The combination feature also appeared when using a shorter fragment from −292 to −154 bp of the CYP6B6 promoter. Taken together, we found a 2-tridecanone core responsive region between −292 and −154 bp of the CYP6B6 promoter. This may lead us to a better understanding of transcriptional mechanism of P450 gene and provide very useful information for the pest control.

Keywords

Helicoverpa armigeracytochrome P450 CYP6B6promoter activity2-tridecanone responsive region
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 104 , Issue 6 , December 2014 , pp. 801 - 808
Copyright
Copyright © Cambridge University Press 2014 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ahmad, M., Arif, M.I. & Ahmad, Z. (2001) Resistance to carbamate insecticides in Helicoverpa armigera (Lepidoptera: Noctuidae) in Pakistan. Crop Protection 20, 427–432.Google Scholar
Bjarnadottir, H. & Jonsson, J.J. (2005) A rapid real-time qRT-PCR assay for ovine β-actin mRNA. Journal of Biotechnology 117, 173–182.Google Scholar
Chen, S., Yang, Y.H. & Wu, Y.D. (2005) Correlation between fenvalerate resistance and cytochrome P450-mediated O-demethylation activity in Helicoverpa armigera (Lepidoptera: Noctuidae). Journal of Economic Entomology 98, 943–946.Google Scholar
Cohen, M.B., Schuler, M.A. & Berenbaum, M.R. (1992) A host-inducible cytochrome P450 from a host-specific caterpillar: molecular cloning and evolution. Proceedings of the National Academy of Sciences 89, 10920–10924.Google Scholar
Cury, J.A. & Koo, H. (2007) Extraction and purification of total RNA from Sreptococcus mutans biofilms. Analytical Biochemistry 365, 208–214.Google Scholar
Denison, M.S. & Nagy, S.R. (2003) Activation of the aryl hydrocarbon receptor by structurally diverse exogenous and endogenous chemicals. Annual Review of Pharmacology and Toxicology 43, 309–334.Google Scholar
Feyereisen, R. (1999) Insect P450 enzymes. Annual Review of Entomology 44, 507–533.Google Scholar
Fujii-Kuriyama, Y. & Mimura, J. (2005) Molecular mechanisms of AhR functions in the regulation of cytochrome P450 genes. Biochemical and Biophysical Research Communications 338, 311–317.Google Scholar
Gerlier, D. & Thomasset, N. (1986) Use of MTT colorimetric assay to measure cell activation. Journal of Immunological Methods 94, 57–63.Google Scholar
Goncalves, M.I.F., Maluf, W.R., Gomes, L.A.A. & Barbosa, L.V. (1998) Variation of 2-tridecanone level in tomato plant leaflets and resistance to two mite species (Tetranychus sp.). Euphytica 104, 33–38.Google Scholar
Harrison, T.L., Zanger, A.R., Schuler, M.A. & Berenbaum, M.R. (2001) Developmental variation in cytochrome P450 expression in Papilio polyxenes in response to xanthotoxin, a hostplant allelochemicals. Archives of Insect Biochemistry and Physiology 48, 179–189.Google Scholar
Harshman, L.G. (1998) Differential gene expression in insects: transcriptional control. Annual Review of Entomology 43, 671–700.Google Scholar
Hung, C.F., Harrison, T.L., Berenbaum, M.R. & Schuler, M.A. (1995) CYP6B3: a second furanocoumarin-inducible cytochrome P450 expressed in Papilio polyxenes . Insect Molecular Biology 4, 149–160.Google Scholar
Hung, C.F., Holzmacher, R., Connolly, E., Berenbaum, M.R. & Schuler, M.A. (1996) Conserved promoter elements in the CYP6B gene family suggest common ancestry for cytochrome P450 monooxygenases mediating furanocoumarin detoxification. Proceedings of the National Academy of Sciences 93, 12200–12205.Google Scholar
Kawajiri, K. & Fujii-Kuriyama, Y. (2007) Cytochrome P450 gene regulation and physiological functions mediated by the aryl hydrocarbon receptor. Archives of Biochemistry and Biophysics 464, 207–212.Google Scholar
Kimps, N.W., Bissnger, B.W., Apperson, C.S., Sonenshine, D.E. & Roe, R.M. (2011) First report of the repellency of 2-tridecanone against ticks. Medical and Veterinary Entomology 25, 202–208.Google Scholar
Li, F. & Liu, X.N. (2012) Optimization of the detection conditions of the promoter activity of Cytochrome P450 CYP6B6 gene from Helicoverpa armigera . Biotechnology bulletin 5, 167–172.Google Scholar
Li, F., Ma, J. & Liu, X.N. (2012) Activity analysis of the promoter region of Helicoverpa armigera P450 CYP6B6 Gene. Journal of Xinjiang University (Natural Science Edition) 29, 13–18.Google Scholar
Li, X.C., Berenbaum, M.R. & Schuler, M.A. (2002) Plant allelochemicals differentially regulate Helicoverpa zea cytochrome P450 genes. Insect Molecular Biology 11, 343–351.Google Scholar
Liu, X.N., Liang, P., Gao, X.W. & Shi, X.Y. (2006) Induction of the cytochrome P450 activity by plant allelochemicals in the cotton bollworm, Helicoverpa armigera (Hübner). Pesticide Biochemistry and Physiology 84, 127–134.Google Scholar
Mantovani, R. (1998) A survey of 178 NF-Y binding CCAAT boxes. Nucleic Acids Research 26, 1135–1143.Google Scholar
McDonnell, C.M., Brown, R.P., Berenbaum, M.R. & Schuler, M.A. (2004) Conserved regulatory elements in the promoters of two allelochemical-inducible cytochrome P450 genes differentially regulate transcription. Insect Biochemistry and Molecular Biology 34, 1129–1139.Google Scholar
Prapaipong, H., Berenbaum, M.R. & Schuler, M.A. (1994) Transcriptional regulation of the Papilio polyxenes CYP6B1 gene. Nucleic Acids Research 22, 3210–3217.Google Scholar
Riskallah, M.R., Dauterman, W.C. & Hodgson, E. (1986) Host plant induction of microsomal monooxygenase activity in relation to Diazinon metabolism and toxicity in larvae of the tobacco budworm Heliothis Virscens (F.). Pesticide Biochemistry and Physiology 25, 233–247.Google Scholar
Schmidt, J.V. & Bradfield, C.A. (1996) Ah receptor signaling pathways. Annual Review of Cell and Developmental Biology 12, 55–89.Google Scholar
Schuler, M.A. (2011) P450s in plant–insect interactions. Biochimica et Biophysica Acta 1814, 36–45.Google Scholar
Scott, J.G. (1999) Cytochrome P450 and insecticide resistance. Insect Biochemistry and Molecular Biology 29, 757–777.Google Scholar
Scott, J.G. (2008) Insect cytochrome P450s: thinking beyond detoxification. Recent Advances in Insect Physiology, Toxicology and Molecular Biology, 117–124.Google Scholar
Vontas, J., Blass, C., Koutsos, A.C., David, J.-P., Kafatos, F.C., Louis, C., Hemingway, J., Christophides, G.K. & Ranson, H. (2005) Gene expression in insecticide resistant and susceptible Anopheles gambiae strains constitutively or after insecticide exposure. Insect Molecular Biology 14, 509–521.Google Scholar
Watson, A.J. & Hankins, O. (1992) Dioxin- and Ah receptor-dependent protein binding to xenobiotic responsive elements and G-rich DNA studied by in Vivo Footprinting. The Journal of Biological Chemistry 266, 6874–6878.Google Scholar
Whitlock, J.P. Jr. (1999) Induction of cytochrome P4501A1 . Annual Review of Pharmacology and Toxicology 39, 103–125.Google Scholar
Williams, W.G., Kennedy, G.G., Yamamoto, R.T., Thacker, J.D. & Bordner, J. (1980) 2-Tridecanone: a naturally occurring insecticide from the wild tomato Lycopersicon hirsutum f. glabratum . Science 207, 888–889.Google Scholar
Willoughby, L., Chung, H., Lumb, C., Robin, C., Batterham, P. & Daborn, P.J. (2006) A comparison of Drosophila melanogaster detoxification gene induction responses for six insecticides, caffeine and Phenobarbital. Insect Biochemistry and Molecular Biology 36, 934–942.Google Scholar
Wu, K.J. & Gong, P.Y. (1997) A new and practical artificial diet for the cotton bollworm. Entomologia sinica 4, 277–282.Google Scholar
Yang, Y., Wu, Y., Chen, S., Devine, G.J., Denholm, I., Jewess, P. & Moores, G.D. (2004) The involvement of microsomal oxidases in pyrethroid resistance in Helicoverpa armigera from Asia. Insect Biochemistry and Molecular Biology 34, 763–773.Google Scholar
Zhou, X.J., Sheng, C.F., Li, M., Wana, H., Liu, D. & Qiu, X.H. (2010) Expression responses of nine cytochrome P450 genes to xenobiotics in the cotton bollworm Helicoverpa armigera . Pesticide Biochemistry and Physiology 97, 209–213.Google Scholar