Hostname: page-component-cd9895bd7-gbm5v Total loading time: 0 Render date: 2024-12-26T16:07:09.596Z Has data issue: false hasContentIssue false
  • English
  • Français

Hybridization between Helicoverpa armigera and Helicoverpa assulta (Lepidoptera: Noctuidae): development and morphological characterization of F1 hybrids

Published online by Cambridge University Press:  09 March 2007

X.-C. Zhao ,
J.-F. Dong ,
Q.-B. Tang ,
Y.-H. Yan ,
I. Gelbic ,
J.J.A. Van Loon  and
C.-Z. Wang
X.-C. Zhao
Affiliation:
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
J.-F. Dong
Affiliation:
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
Q.-B. Tang
Affiliation:
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
Y.-H. Yan
Affiliation:
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
I. Gelbic
Affiliation:
Department of Morphology, Institute of Entomology AS CR, 370 05 Ceske Budejovice, Czech Republic
J.J.A. Van Loon
Affiliation:
Laboratory of Entomology, Wageningen University, Wageningen, The Netherlands
C.-Z. Wang*
Affiliation:
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
*
*Fax: + 86 10 6256 5689 E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Reciprocal hybridizations between Helicoverpa armigera (Hübner) and Helicoverpa assulta (Guenée) were studied. The cross between females of H. armigera and males of H. assulta yielded only fertile males and sterile individuals lacking an aedeagus, valva or ostium bursae. A total of 492 larvae of the F1 generation were obtained and 374 of these completed larval development and pupated. Only 203 pupae were morphologically normal males, the remaining 171 pupae were malformed. Larvae and pupae that gave rise to morphologically abnormal adults exhibited longer development times. Sterility was not only associated with malformed external sex organs, but also a range of abnormalities of the internal reproductive system: (i) loss of internal reproductive organs, (ii) with one to three copies of an undeveloped bursa copulatrix; or (iii) with one or two undeveloped testes. Normal male hybrid adults showed higher flight activity in comparison with males of both species. In contrast, the cross between females of H. assulta and males of H. armigera yielded morphologically normal offspring (80 males and 83 females). The interaction of the Z-chromosome from H. assulta with autosomes from H. armigera might result in morphological abnormalities found in hybrids and backcrosses, and maternal-zygotic incompatibilities might contribute to sex bias attributed to hybrid inviability.

Keywords

Helicoverpa armigeraHelicoverpa assultaNoctuidaeLepidopterainterspecific hybridmorphologysterility
Type
Research Article
Information
Bulletin of Entomological Research , Volume 95 , Issue 5 , October 2005 , pp. 409 - 416
Copyright
Copyright © Cambridge University Press 2005

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Chen, Y.X. (1999) Fauna Sinica Vol. 16: Insecta, Lepidoptera, Noctuidae. Beijing, Science Press. 145–147(in Chinese).Google Scholar
Cheng, D.F., Tian, Z., Li, H.M., Sun, J.R. & Chen, J.L. (2002) Influence of temperature and humidity on the flight capacity of Sitobion avenae. Acta Entomologica Sinica 45, 80–85.Google Scholar
Coyne, J.A. & Orr, H.A. (1989) Patterns of speciation in Drosophila. Evolution 43, 362–381.CrossRefGoogle ScholarPubMed
Degrugillier, M.E., Newman, S.M. Jr. (1993) Hereditary viruses of Heliothis ? Chromatin-associated virus-like particles in testes of six species of Heliothis and Helicoverpa, F 1, and backcross males. Journal of Invertebrate Pathology 61, 147–155.CrossRefGoogle Scholar
Downes, J.A. (1959) The gypsy moth and some possibilities of the control of insects by genetical means. Canadian Entomologist 91, 661–664.CrossRefGoogle Scholar
Fitt, G.P. (1989) The ecology of Heliothis spp. in relation to agroecosystems. Annual Review of Entomology 34, 17–52.CrossRefGoogle Scholar
Goodpasture, C., LaChance, L.E. & Richard, R.D. (1980a) Persistence of abnormal spermiogenesis in the backcross generations of interspecific hybrids between Heliothis virescens × H. subflexa. Annals of the Entomological Society of America 73, 397–403.CrossRefGoogle Scholar
Goodpasture, C., Richard, R.D., Martin, D. & Laster, M. (1980b) Sperm cell abnormalities in progeny from interspecific crosses between Heliothis virescens and H. subflexa. Annals of the Entomological Society of America 73, 529–532.CrossRefGoogle Scholar
Grula, J.W. & Taylor, O.R. (1980) Some characteristics of hybrids derived from the sulfur butterflies, Colias eurytheme and C. philodice: phenotypic effects of the X-chromosome. Evolution 34, 673–687.Google Scholar
Hardwick, D.F. (1965) The corn earworm complex. Memoirs of the Entomological Society of Canada 40, 14–19.Google Scholar
Jiggins, C.D., Linares, M., Naisbit, R.E., Salazar, C., Yang, Z.H. & Mallet, J. (2001) Sex-linked hybrid sterility in a butterfly. Evolution 55, 1631–1638.Google Scholar
Knipling, E.F. (1960) Use of insects for their own destruction. Journal of Economic Entomology 53, 415–420.CrossRefGoogle Scholar
Krueger, C.M., Degrugillier, M.E. & Narang, S.K. (1993) Size difference among 16S rRNA genes from endosymbiotic bacteria found in testes of Heliothis virescens, H. subflexa (Lepidoptera: Noctuidae), and backcross sterile male moths. Florida Entomologist 76, 382–383.CrossRefGoogle Scholar
LaChance, L.E. (1984) Hybrid sterility: eupyrene sperm production and abnormalities in the backcross generations of interspecific hybrids between Heliothis subflexa and H. virescens (Lepidoptera: Noctuidae). Annals of the Entomological Society of America 77, 93–101.CrossRefGoogle Scholar
LaChance, L.E. & Karpenko, C.P. (1983) Hybrid sterility in Heliothis subflexa × H. virescens (Lepidoptera: Noctuidae) crosses: expression after injection with antiviral agents, heat shocks, and rearing at extreme temperatures. Annals of the Entomological Society of America 76, 104–109.CrossRefGoogle Scholar
Laster, M.L. (1972) Interspecific hybridization of Heliothis virescens and H. subflexa. Environmental Entomology 6, 682–687.CrossRefGoogle Scholar
Laster, M.L. & Hardee, D.D. (1995) Intermating compatibility between North American Helicoverpa zea and Heliothis armigera (Lepidoptera: Noctuidae) from Russia. Journal of Economic Entomology 88, 77–80.CrossRefGoogle Scholar
Laster, M.L. & Sheng, C.F. (1995) Search for hybrid sterility for Helicoverpa zea in crosses between the North American H. zea and H. armigera (Lepidoptera: Noctuidae) from China. Journal of Economic Entomology 88, 1288–1291.CrossRefGoogle Scholar
Laster, M.L., King, E.G. & Furr, R.E. (1988) Interspecific hybridization of Heliothis subflexa and H. virescens (Lepidoptera: Noctuidae) from Argentina. Environmental Entomology 17, 1016–1018.CrossRefGoogle Scholar
Laurie, C.C. (1997) The weaker sex is heterogametic: 75 years of Haldanes rule. Genetics 147, 937–951.CrossRefGoogle ScholarPubMed
Liu, M.Y., Cai, J.P. & Tian, Y. (1994) Sex pheromone components of the oriental tobacco budworm, Helicoverpa assulta Guenée: identification and field trials. Entomologica Sinica 1, 77–5.Google Scholar
Miller, S.G. & Miller, R.D. (1996) Infectious enterococcus from Heliothis virescens × H. subflexa backcross hybrids (Lepidoptera: Noctuidae). Annals of the Entomological Society of America 89, 420–427.CrossRefGoogle Scholar
Miller, S.G., Huettel, M.D., Davis, M.T.B., Weber, E.H. & Weber, L.A. (1986) Male sterility in Heliothis virescens × H. subflexa backcross hybrids, evidence for abnormal mitochondrial transcripts in testes. Molecular Genetics and Genomics 203, 451–461.CrossRefGoogle Scholar
Park, K.C., Cork, A. & Boo, K.S. (1996) Intrapopulational changes in sex pheromone composition during scotophase in oriental tobacco budworm, Helicoverpa assulta (Guenée) (Lepidoptera: Noctuidae). Journal of Chemical Ecology 22, 1201–1210.CrossRefGoogle ScholarPubMed
Presgraves, D.C. (2002) Patterns of postzygotic isolation in Lepidoptera. Evolution 56, 1168–1183.Google ScholarPubMed
Proshold, F.I. (1983) Release of backcross insects on St. Croix, U.S. Virgin Islands, to suppress the tobacco budworm (Lepidoptera: Noctuidae): infusion of sterility into a native population. Journal of Economic Entomology 76, 1353–1359.CrossRefGoogle Scholar
Proshold, F.I. & LaChance, L.E. (1974) Analysis of sterility in hybrids from interspecific crosses between Heliothis virescens and H. subflexa. Annals of the Entomological Society of America 67, 445–449.CrossRefGoogle Scholar
Richard, R.D., LaChance, L.E. & Proshold, F.I. (1974) An ultrastructural study of sperm in sterile hybrids from crosses of Heliothis virescens and H. subflexa. Annals of the Entomological Society of America 67, 35–39.Google Scholar
SPSS (2001) SPSS 11.01 for Windows. SPSS Inc, Chicago, Illinois.Google Scholar
Turelli, M. & Orr, H.A. (2000) Dominance, epistasis and the genetics of postzygotic isolation. Genetics 154, 1663–1679.CrossRefGoogle ScholarPubMed
Wang, C.Z. & Dong, J.F. (2001) Interspecific hybridization of Helicoverpa armigera and H. assulta (Lepidoptera: Noctuidae). Chinese Science Bulletin 46, 489–491.CrossRefGoogle Scholar
Wu, D.M., Yan, Y.H. & Cui, J.R. (1997) Sex pheromone components of Helicoverpa armigera: chemical analysis and field tests. Entomologica Sinica 4, 350–56.Google Scholar
Wu, K.J. & Gong, P.Y. (1997) A new and practical artificial diet for the cotton bollworm. Entomologica Sinica 4, 277–282.Google Scholar
Wu, W.Q., Tang, X.H., Xu, S.F. & Du, J.W. (1990) Diel periodicity of female calling activity and sex pheromone production in Heliothis armigera (Lepidoptera: Noctuidae). Contributions from Shanghai Institute of Entomology 10, 57–62.Google Scholar