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Glossina swynnertoni (Diptera: Glossinidae): effective population size and breeding structure estimated by mitochondrial diversity

Published online by Cambridge University Press:  09 March 2007

J.G. Marquez ,
I.I. Malele ,
J.O. Ouma  and
E.S. Krafsur
J.G. Marquez
Affiliation:
Department of Entomology, Iowa State University, Ames, Iowa 50011, USA
I.I. Malele
Affiliation:
Tsetse and Trypanosomiasis Research Institute, Tanga, Tanzania
J.O. Ouma
Affiliation:
Department of Entomology, Iowa State University, Ames, Iowa 50011, USA
E.S. Krafsur*
Affiliation:
Department of Entomology, Iowa State University, Ames, Iowa 50011, USA
*
*Fax: 515 294 5957 E-mail: [email protected]
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Abstract

Nucleotide diversity was examined at mitochondrial COI and r16S2 loci in eight Glossina swynnertoni Austen collections from northern Tanzania and from a culture maintained by the International Atomic Energy Agency. Eighteen composite haplotypes were observed among 149 flies, two of which were common to all samples and 10 were private. Mean haplotype diversity was 0.59 and nucleotide diversity was 0.0013. There were excess singular haplotypes and mutation-drift disequilibrium suggesting that populations had experienced an earlier bottleneck and subsequent expansion. Factorial correspondence analysis showed that haplotype frequencies varied much more temporally (GST = 0.18) than spatially (GST = 0.04). The estimate of effective population size Ne in Tarangire was a harmonic mean ~50 reproductive flies averaged over ~47 generations. The mean rate of gene flow was estimated to be ~5±1 reproducing females per generation but inflated because of mutation-drift disequilibrium arising from likely earlier bottlenecks.

Keywords

gene floweffective population sizemitochondrial variationtsetse
Type
Research Article
Information
Bulletin of Entomological Research , Volume 96 , Issue 4 , August 2006 , pp. 353 - 360
Copyright
Copyright © Cambridge University Press 2006

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References

Benzecri, J.-P. (1992) Correspondence analysis handbook. 665 pp. New York, Marcel Dekker.CrossRefGoogle Scholar
Buxton, P.A. (1955) The natural history of tsetse flies: an account of the biology of the genus Glossina (Diptera). 816 pp. London, H.K. Lewis & Co.Google Scholar
Charlesworth, B. (2002) Effective population size. Current Biology 12, R716–717.CrossRefGoogle ScholarPubMed
Challier, A. & Laveissière, C. (1973) Un nouveau piège pour la capture des glossines (Glossina; Diptera, Muscidae): description et essays sur le terrain. Cahiers ORSTOM, Entomologie Médicale et Parasitologie 11, 251–262.Google Scholar
Ford, J. (1971) The role of trypanosomiases in African ecology. 568 pp. Oxford, Clarendon Press.Google Scholar
Fu, Y.-X. (1997) Statistical tests of neutrality of mutations against population growth, hitchhiking and background selection. Genetics 147, 915–925.CrossRefGoogle ScholarPubMed
Fu, Y.-X & Li, W.H. (1993) Statistical tests of neutrality of mutations. Genetics 133, 693–709.CrossRefGoogle ScholarPubMed
Gooding, R.H. & Krafsur, E.S. (2005) Tsetse genetics: contributions to biology, systematics, and control of tsetse flies. Annual Review of Entomology 50, 101–123.CrossRefGoogle ScholarPubMed
Gooding, R.H., Mbise, S., Macha, P. & Rolseth, B.M. (1993) Genetic variation in a Tanzanian population of Glossina swynnertoni (Diptera: Glossinidae). Journal of Medical Entomology 30, 489–492.CrossRefGoogle Scholar
Greenacre, M.J. (1993) Correspondence analysis in practice. 195 pp. London, Academic Press.Google Scholar
Hall, T.A. (1999) BioEdit:, a, user-friendly, biological, sequence, alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41, 95–98.Google Scholar
Hargrove, J.W. (2000) A theoretical study of the invasion of cleared areas by tsetse flies (Diptera: Glossinidae). Bulletin of Entomological Research 90, 201–209.CrossRefGoogle ScholarPubMed
Hargrove, J.W. (2003) Tsetse eradication: sufficiency, necessity and desirability. Research report, DFID Animal Health Programme, Centre for Tropical Veterinary Medicine, University of Edinburgh, UK.Google Scholar
Hocking, K.S., Lee, C.W., Beesley, J.S.S. & Matechi, H.T. (1966) Aircraft applications of insecticides in East Africa. XVI – airspray experiment with endosulfan against Glossina morsitans Westw., G. swynnertoni Aust. and G. pallidipes Aust. Bulletin of Entomological Research 56, 737–744.CrossRefGoogle Scholar
Jordan, A.M. (1986) Trypanosomiasis control and African rural development. 357 pp. Harlow, Essex, Longman Group Ltd.Google Scholar
Krafsur, E.S. (2003) Tsetse fly population genetics: an indirect approach to dispersal. Trends in Parasitology 19, 162–166.CrossRefGoogle ScholarPubMed
Krafsur, E.S. & Griffiths, N. (1997) Genetic variation at structural loci in the Glossina morsitans species group. Biochemical Genetics 35, 1–11.CrossRefGoogle ScholarPubMed
Kumar, S., Tamura, K. & Nei, M. (2004) MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Briefings in Bioinformatics 5, 150–163.CrossRefGoogle ScholarPubMed
Luikart, G., Allendorf, F.W., Cornuet, J.-M. & Sherwin, W.B. (1999) Distortion of allele frequency distributions provides a test for recent population bottlenecks. Journal of Heredity 89, 238–247.CrossRefGoogle Scholar
Ndegwa, P.N. & Mihok, S. (1999) Development of odour-baited traps for Glossina swynnertoni (Diptera: Glossinidae). Bulletin of Entomological Research 89, 255–261.CrossRefGoogle Scholar
Nei, M. (1987) Molecular evolutionary genetics. 512 pp. New York, Columbia University Press.CrossRefGoogle Scholar
Nei, M. & Kumar, S. (2000) Molecular evolution and phylogenetics. 333 pp. New York, Oxford University Press Inc.CrossRefGoogle Scholar
Potts, W.H. (1937) The distribution of tsetse-fies in Tanganyika territory. Bulletin of Entomological Research 28, 129–148.CrossRefGoogle Scholar
Ramos-Onsins, S.E. & Rozas, J. (2002) Statistical properties of new neutrality tests against population growth. Molecular Biology and Evolution 19, 2092–2100.CrossRefGoogle ScholarPubMed
Ripamonti, D., Massari, M., Arici, C., Gabbi, E., Farina, C. & Brini, M. (2002) African sleeping sickness in tourists returning from Tanzania: the first 2 Italian cases from a small outbreak among European travelers. Clinical and Infectious Diseases 34, E18–E22.CrossRefGoogle ScholarPubMed
Rogers, D.J. & Boreham, P.F. (1973) Sleeping sickness survey in the Serengeti area (Tanzania) 1971. II. The vector role of Glossina swynnertoni Austen. Acta Tropica 30, 24–35.Google ScholarPubMed
Rogers, D.J. & Robinson, T.P. (2004) Tsetse distribution. pp. 139–179 in Maudlin, I., Holmes, P.H. & Miles, M.A. (Eds) The trypanosomes. Wallingford, Oxon, CABI Publishing.CrossRefGoogle Scholar
Rozas, J., Sánchez-DelBarrio, J.C., Messeguer, X. & Rozas, R. (2003) DnaSP, DNA polymorphism analyses by the coalescent and other methods. Bioinformatics 19, 2496–2497.CrossRefGoogle ScholarPubMed
SAS (2003) SAS language reference, Version 9.2. Cary North Carolina.Google Scholar
Simon, C., Frati, F., Beckenbach, A., Crespi, B., Liu, H. & Flook, P. (1994) Evolution, weighting, and phylogenetic utility of mitochondrial gene sequence and a compilation of conserved polymerase chain reaction primers. Annals of the Entomological Society of America 87, 651–701.CrossRefGoogle Scholar
Slatkin, M. & Hudson, R.R. (1991) Pairwise comparisons of mitochondrial DNA sequences in stable and exponentially growing populations. Genetics 129, 555–562.CrossRefGoogle ScholarPubMed
Stiles, J.K., Otieno, L.H., Chaudhury, M.F. & Moloo, S.K. (1994) Upsurge of the tsetse fly Glossina swynnertoni at Nguruman, Kenya. Medical and Veterinary Entomology 8, 199–200.CrossRefGoogle ScholarPubMed
Swynnerton, C.F.M. (1936) The tsetse flies of East Africa: a first study of their ecology with a view to their control. Transactions of the Royal Entomological Society of London 84, 1–579.Google Scholar
Thompson, J.D., Gibson, T.L., Plewniak, F., Jeanmougin, F. & Higgins, D.G. (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 24, 4876–4882.CrossRefGoogle Scholar
Torr, S.J., Hargrove, J.W. & Vale, G.A. (2005) Towards a rational policy for dealing with tsetse. Trends in Parasitology 21, 537–541.CrossRefGoogle ScholarPubMed
Wright, S. (1931) Evolution in Mendelian populations. Genetics 16, 97–159.CrossRefGoogle ScholarPubMed