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Genetic variability in esterases and the insecticide resistance in brazilian strains of Oryzaephilus mercator and Oryzaephilus surinamensis (Coleoptera: Silvanidae)

Published online by Cambridge University Press:  01 November 2010

G.A.R. Silva  and
A.S. Lapenta
G.A.R. Silva*
Affiliation:
Departamento de Biologia Celular e Genética, Universidade Estadual de Maringá, Avenida Colombo 5790, 87020-900, Maringá-Pr, Brasil
A.S. Lapenta
Affiliation:
Departamento de Biologia Celular e Genética, Universidade Estadual de Maringá, Avenida Colombo 5790, 87020-900, Maringá-Pr, Brasil
*
*Author for correspondence Fax: (44) 30114893 E-mail: [email protected]
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Abstract

Oryzaephilus mercator and O. surinamensis are stored grains and processed food pests, the latter being responsible for major economical losses. Polyacrylamide gel electrophoresis was used to analyse esterase patterns during insect development. Seven esterases, three cholinesterases, two carboxylesterases and two acetylesterases, were identified in O. mercator, one of which was proper to adults. Five esterases, of which four were cholinesterases, occurred in O. surinamensis. Strains of O. mercator and O. surinamensis were also exposed to malathion and chlorpyrifos-methyl. According to the LC50 estimates, OmLC-M and OmLA strains of O. mercator and OsLB strain of O. surinamensis were the most resistant to both insecticides. However, higher sensitivity to malathion and chlorpyrifos-methyl has also been verified in some of its esterases. Cholinesterases OmEST-1 and OsEST-5 seem to be involved in this resistance. These results suggest that organophosphate tolerance may be related to genetic variability in esterase isoenzymes.

Keywords

Oryzaephilus mercatorOryzaephilus surinamensisesterasesresistanceinsecticidesorganophosphates
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 101 , Issue 2 , April 2011 , pp. 177 - 185
Copyright
Copyright © Cambridge University Press 2010

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