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Genetic diversity of an introduced pest, the green spruce aphid Elatobium abietinum (Hemiptera: Aphididae) in New Zealand and the United Kingdom

Published online by Cambridge University Press:  10 July 2009

D. Nicol ,
K.F. Armstrong ,
S.D. Wratten ,
P.J. Walsh ,
N.A. Straw ,
C.M. Cameron ,
C. Lahmann  and
C.M. Frampton
D. Nicol*
Affiliation:
Soil, Plant and Ecological Sciences Division, PO Box 84, Lincoln University, Canterbury, New Zealand
K.F. Armstrong
Affiliation:
Soil, Plant and Ecological Sciences Division, PO Box 84, Lincoln University, Canterbury, New Zealand
S.D. Wratten
Affiliation:
Soil, Plant and Ecological Sciences Division, PO Box 84, Lincoln University, Canterbury, New Zealand
P.J. Walsh
Affiliation:
Forest Research Associates Ltd, PO Box 1031, Rotorua, New Zealand
N.A. Straw
Affiliation:
Forestry Research, Alice Holt Lodge, Wrecclesham, Farnham, Surrey, GL10 4LH, UK
C.M. Cameron
Affiliation:
Soil, Plant and Ecological Sciences Division, PO Box 84, Lincoln University, Canterbury, New Zealand
C. Lahmann
Affiliation:
Soil, Plant and Ecological Sciences Division, PO Box 84, Lincoln University, Canterbury, New Zealand
C.M. Frampton
Affiliation:
Applied Management and Computing Division, PO Box 84, Lincoln University, Canterbury, New Zealand
*
*+64 3 325 3844[email protected]
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Abstract

The green spruce aphid Elatobium abietinum (Walker) is an introduced pest in the United Kingdom and more recently in New Zealand. In outbreak years this aphid can cause severe defoliation and sometimes death of spruce trees (Picea spp.). As chemical control is not financially viable, other options including host-plant resistance and biological control are currently being investigated. An understanding of the genetic variation of this pest is imperative in fully utilizing these control strategies. To examine this, E. abietinum was collected from Sitka spruce Picea sitchensis from four locations in the UK that were up to 240 km apart. Of these, 40 aphids were analysed via two alternative polymerase chain reaction (PCR) analyses using primer pairs. The first analysis used 10-mer random primers, whilst the second analysis used primers designed to amplify across the intergenic spacer region of rDNA. Combining results from the two analyses allowed the 40 UK aphids to be separated into 28 different genotypes. The genetic variation was also high within each UK site, with 77 to 89% of the aphids sampled being of a different genotype. The two PCR analyses were subsequently used to examine 40 aphids across six sites in New Zealand up to 1200 km apart. No genetic variation was identified. Further analysis of several of these New Zealand aphids with 87 individual 10-mer primers and two polymerase enzymes, still did not detect any genetic variation. The high degree of genotypic diversity in the UK populations was presumably due to a longer period of establishment, multiple introductions and/or sexual reproduction. The contrasting lack of genetic variation in New Zealand populations was probably due to a very limited founder population, continued isolation and lack of sexual reproduction. Reduced genetic diversity can seriously decrease the ability of a population to adapt to control strategies. Therefore the durability of certain control methods may be more readily maintained in such an isolated population in New Zealand.

Type
Review Article
Information
Bulletin of Entomological Research , Volume 88 , Issue 5 , October 1998 , pp. 537 - 543
Copyright
Copyright © Cambridge University Press 1998

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References

Armstrong, K.A. & Wratten, S.D. (1996) The use of DNA analysis and the polymerase chain reaction in the study of introduced pests in New Zealand. pp. 231–263in Symondson, W.O.C. & Liddell, J.E. (Eds) The ecology of agricultural pests: biochemical approaches, London, Chapman & Hall.Google Scholar
Bevan, D. (1966) The green spruce aphis Elatobium (Neomyzaphis) abietinum Walker. Scottish Forestry 20, 193–201.Google Scholar
Blackman, R.L. & Eastop, V.F. (1994) Aphids on the world's trees: an identification and information guide. Wallingford, UK, CAB International.Google Scholar
Carter, C.I. (1989) The 1989 outbreak of the green spruce aphid, Elatobium abietinum. Forestry Commission Research Information Note 161. London, HMSO.Google Scholar
Carter, C.I. & Austara, O. (1994) The occurrence of males, oviparous females and eggs within anholocyclic populations of the green spruce aphid Elatobium abietinum (Walker) (Hemiptera: Aphididae). Norwegian Journal of Entomology 41, 53–58.Google Scholar
Close, R.C. & Tomlinson, A.I. (1975) Dispersal of the grain aphid Macrosiphum miscanthi from Australia to New Zealand. The New Zealand Entomologist 6, 62–65.CrossRefGoogle Scholar
Cockayne, A.H. (1911) An insect injurious to spruce trees. New Zealand Journal of the Department of Agriculture 2, 117–120.Google Scholar
De Barro, P.J. (1994) Multilocus DNA fingerprinting of clonal insects: its potential uses in cereal aphid ecology. IOBC–WPRS Bulletin 17, 1–10.Google Scholar
De Barro, P.J., Sherratt, T.N., Carvalho, G.R., Nicol, D., Iyengar, A. & Maclean, N. (1994) An analysis of secondary spread by putative clones of Sitobion avenae within a Hampshire wheat field using the multilocus (GATA)4 probe. Insect Molecular Biology 3, 253–260.Google Scholar
De Barro, P.J., Sherratt, T.N., Carvalho, G.R., Nicol, D., Iyengar, A. & Maclean, N. (1995) The use of the multilocus (GATA)4 probe to investigate geographic and micro-geographic genetic variation in two aphid species over Southern England. Molecular Ecology 4, 375–382.Google Scholar
Dumbleton, L.J. (1932) Report on spruce aphis investigation for the year ending December 1930. New Zealand Journal of Science and Technology 13, 207–220.Google Scholar
Ender, A., Schwenk, K., Stadler, T., Streit, B. & Schierwater, B. (1996) RAPD identification of microsatellites in Daphnia. Molecular Ecology 5, 437–442.CrossRefGoogle ScholarPubMed
Evans, J.W. (1943) Insect pests and their control. Tasmania, HH Pimblett.Google Scholar
Frankham, R. (1995a) Inbreeding and extinction: a threshold effect. Conservation Biology 9, 792–799.CrossRefGoogle Scholar
Frankham, R. (1995b) Conservation genetics. Annual Review of Genetics 29, 305–327.Google Scholar
Hales, D.F., Tomiuk, J., Wöhrmann, K. & Sunnucks, P. (1997) Evolutionary and genetic aspects of aphid biology: a review. European Journal of Entomology 94, 1–55.Google Scholar
Jones, J.S. (1980) Can genes choose habitats? Nature 286, 757–758.CrossRefGoogle ScholarPubMed
Loxdale, H.D., Hardie, J., Halbert, S., Foottit, R., Kidd, N.A.C. & Carter, C.I. (1993) The relative importance of short- and long-range movement of flying aphids. Biological Review 68, 291–311.Google Scholar
Matioli, S.R. & de Brito, R.A. (1995) Obtaining genetic markers by using double-stringency PCR with microsatellites and arbitrary primers. Biotechniques 19, 752–758.Google ScholarPubMed
Miller, D. (1925) Forest and timber insects in New Zealand. New Zealand State Forest Service Bulletin No. 2.Google Scholar
Miller, J.T. & Knowles, F.B. (1989) Introduced forest trees in New Zealand: recognition, role and seed source. 6. The spruces. New Zealand Forest Research Institute Bulletin No. 124.Google Scholar
Mitchell, A. (1974) A field guide to the trees of Britain and northern Europe. London, Collins.Google Scholar
Montgomery, G.W. & Sise, J.A. (1990) Extraction of DNA from sheep white blood cells. New Zealand Journal of Agricultural Research 33, 437–441.CrossRefGoogle Scholar
Myers, J.G. (1922) The order Hemiptera in New Zealand. New Zealand Journal of Science and Technology 5, 1–12.Google Scholar
Nicol, D., Armstrong, K.F., Wratten, S.D., Cameron, C.M., Frampton, C.M. & Fenton, B. (1997a) Genetic variation in an introduced aphid pest (Metopolophium dirhodum) in New Zealand and relation to individuals from Europe. Molecular Ecology 6, 255–265.Google Scholar
Nicol, D., Armstrong, K.F., Wratten, S.D., Cameron, C.M. & Lahmann, C. (1997b) Genetic diversity of two introduced aphid species in New Zealand. Proceedings of the 50th New Zealand Weed and Pest Control Conference, pp. 322–326.Google Scholar
Puterka, G.J., Black, W.C., Steiner, W.M. & Burton, R.L. (1993) Genetic variation and phylogenetic relationships among worldwide collections of the Russian wheat aphid, Diuraphis noxia (Mordvilko), inferred from allozyme and RAPD-PCR markers. Heredity 70, 604–618.CrossRefGoogle ScholarPubMed
Shufran, K.A., Black, W.C. & Margolies, D.C. (1991) DNA fingerprinting to study spatial and temporal distributions of an aphid Schizaphis graminum (Homoptera: Aphididae). Bulletin of Entomological Research 81, 303–313.CrossRefGoogle Scholar
Shufran, K.A., Peters, D.C. & Webster, J.A. (1997) Generation of clonal diversity by sexual reproduction in the greenbug, Schizaphis graminum. Insect Molecular Biology 6, 203–209.CrossRefGoogle ScholarPubMed
Sneath, P.H.A. & Sokal, R.R. (1973) Numerical taxonomy: the principle and practice of numerical classification. San Francisco, W.H. Freeman.Google Scholar
Sokal, R.R., Rohlf, F.J. (1981) Biometry, 2nd edn.New York, W.H. Freeman & Co.Google Scholar
Straw, N.A. (1995) Climate change and impact of green spruce aphid, Elatobium abietinum (Walker), in the UK. Scottish Forestry 49, 134–145.Google Scholar
Sunnucks, P., England, P.R., Taylor, A.C. & Hales, D.F. (1996) Microsatellite and chromosome evolution of parthenogenetic Sitobion aphids in Australia. Genetics 144, 747–756.Google Scholar
Sunnucks, P., De Barro, P.J., Lushai, G., Maclean, N. & Hales, D. (1997) Genetic structure of an aphid studied using microsatellites: cyclic parthenogenesis, differentiated lineages and host specialisation. Molecular Ecology 6, 1059–1073.Google Scholar
Theobald, F.V. (1914) Notes on the green spruce aphid (Aphis abietina Walker). Annals of Applied Biology 1, 22–36.Google Scholar
Wallner, W.E. (1987) Factors affecting insect population dynamics: differences between outbreak and non-outbreak species. Annual Review of Entomology 32, 317–340.Google Scholar
Welsh, J. & McClelland, M. (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Research 18, 7213–7218.Google Scholar
Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A. & Tingey, S.V. (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18, 6531–6535.Google Scholar
Zondag, R. (1983) Elatobium abietinum (Walker) (Hemiptera: Aphididae) Spruce Aphid. Forest and Timber Insects in New Zealand No. 54, 4 pp.Google Scholar