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The formulation of the essential oil of Piper aduncum Linnaeus (Piperales: Piperaceae) increases its efficacy as an insect repellent

Published online by Cambridge University Press:  07 November 2016

S.N.H. Mamood ,
O. Hidayatulfathi ,
S.B. Budin ,
G. Ahmad Rohi  and
M.H. Zulfakar
S.N.H. Mamood
Affiliation:
School of Diagnostics & Applied Health Sciences, Faculty of Health Sciences, National University of Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia
O. Hidayatulfathi*
Affiliation:
School of Diagnostics & Applied Health Sciences, Faculty of Health Sciences, National University of Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia
S.B. Budin
Affiliation:
School of Diagnostics & Applied Health Sciences, Faculty of Health Sciences, National University of Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia
G. Ahmad Rohi
Affiliation:
School of Diagnostics & Applied Health Sciences, Faculty of Health Sciences, National University of Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia
M.H. Zulfakar
Affiliation:
Faculty of Pharmacies, National University of Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia
*
*Author for correspondence Phone: 6012-3736549/603-92897693 Fax: +60326929032 E-mail: [email protected]
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Abstract

The essential oil (EO) of Piper aduncum Linnaeus, known as ‘sireh lada’ to locals Malaysian, has the potential to be used as an alternative to synthetic insect repellents such as N,N-diethyl-meta-toluamide. However, the EO's efficacy as a repellent decreases after application due to the high volatility of its active ingredients. A number of studies have showed that optimizing the formulation of plant-based EOs can improve their efficacy as repellents. The present study sought to evaluate the effectiveness of 10% P. aduncum EO in ethanol and in three different semisolid formulations: ointment, cream and gel. These formulations were tested on Aedes aegypti under laboratory conditions. Each formulation was applied to the subject's hands, which were then inserted into a cage containing 25 nulliparous A. aegypti. The number of mosquitoes landing on or biting each subject's hand was recorded, and the repellency percentage, landing/biting percentage and protection time for each of the formulations were compared. There were no statistically significant differences between the semisolid EO formulations with regards to the repellency percentage and the landing/biting percentage at 4 h post-application. All three semisolid EO formulations were able to repel >65% of the A. aegypti at 4 h post-application. The EO ointment formulation provided a protection time (182.5 ± 16.01 min) that was statistically significantly longer than that associated with the EO gel formulation (97.5 ± 14.93 min). Meanwhile, the EO cream formulation provided a protection time of 162.5 ± 6.29 min. As the EO cream and ointment formulations displayed better repellent properties than the EO gel formulation, they appear to be the most promising P. aduncum EO formulations to be developed and commercialized as alternatives to synthetic repellents.

Keywords

Aedes aegyptiPiper aduncumN,N-diethyl-meta-toluamideointmentcreamgel
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 107 , Issue 1 , February 2017 , pp. 49 - 57
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Copyright
Copyright © Cambridge University Press 2016 

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References

Aisien, M.S.O., Imasuen, A.A., Wagbatsomaand, V.A. & Ayinde, B.A. (2004) Preliminary evaluation of the repellent activity of some essential oils against Simulium damnosum s.l., the vector of human onchocerciasis. International Journal of Tropical Insect Science 24(2), 196–199.CrossRefGoogle Scholar
Amer, A. & Mehlhorn, H. (2006) Repellency effect of forty-one essential oils against Aedes, Anopheles, and Culex mosquitoes. Parasitology Research 99(4), 478–490.CrossRefGoogle ScholarPubMed
Barnard, D.R. (2000) Global Collaboration for Development of Pesticides for Public Health: Repellents and Toxicants for Personal Protection: a WHO Position Paper. Geneva, World Health Organization.Google Scholar
Bissinger, B.W., Schmidt, J.P., Owens, J.J., Mitchell, S.M. & Kennedy, M.K. (2014) Performance of the plant-based repellent TT-4302 against mosquitoes in the laboratory and field and comparative efficacy to 16 mosquito repellents against Aedes aegypti (Diptera: Culicidae). Journal of Medical Entomology 51(2), 392–399.CrossRefGoogle ScholarPubMed
Blackwell, A., Stuart, A.E. & Estambale, B.A. (2003) The repellent and antifeedant activity of Myrica gale oil against Aedes aegypti mosquitoes and its enhancement by the addition of salicyluric acid. Journal of the Royal College of Physicians of Edinburgh 33, 209–214.Google Scholar
Browne, L.B. (1997) Host-related responses and their suppression: some behavioral considerations. pp. 117–127 in Shorey, H.H. & McKelvey, J.J. Jr. (Eds) Chemical Control of Insect Behavior. New York, Wiley.Google Scholar
Burger, W.C. (1971) Piperaceae. Fieldiana (Botany) 35, 79–96.Google Scholar
Choochote, W., Chaithong, U., Kamsuk, K., Jitpakdi, A., Tippawangkosol, P., Tuetun, B., Champakaew, D. & Pitasawat, B. (2007) Repellent activity of selected essential oils against Aedes aegypti . Fitoterapia 78(5), 359–364.CrossRefGoogle ScholarPubMed
Chou, J.T., Rossignol, P.A. & Ayres, J.W. (1997) Evaluation of commercial insect repellents on human skin against Aedes aegypti (Diptera: Culicidae). Journal of Medical Entomology 34(6), 624–630.CrossRefGoogle ScholarPubMed
Ciccio, J.F. & Ballestero, C.M. (1997) Constituyentes volátiles de las hojas y espigas de Piper aduncum (Piperaceae) de Costa Rica. Revista de Biologia Tropical 45, 783–790.Google Scholar
Das, M.K. & Ansari, M.A. (2003) Evaluation of repellent action of Cymbopogan martinii Stapf var sofia oil against Anopheles sundaicus in tribal villages of Car Nicobar Island, Andaman & Nicobar Islands, India. Journal of Vector Borne Diseases 40(3–4), 101–104.Google ScholarPubMed
Florence, A.T. & Attwood, D. (1990) Physicochemical Principles of Pharmacy. 2nd edn. Basingstoke, Macmillan Press.Google Scholar
Fradin, M.S. & Day, J.F. (2002) Comparative efficacy of insect repellents against mosquito bites. New England Journal of Medicine 347(1), 13–18.CrossRefGoogle ScholarPubMed
Frances, S.P. & Wirtz, R.A. (2005) Repellents: past, present, and future. Journal of the American Mosquito Control Association 21, 1–3.CrossRefGoogle ScholarPubMed
Gillij, Y.G., Gleiser, R.M. & Zygadlo, J.A. (2008) Mosquito repellent activity of essential oils of aromatic plants growing in Argentina. Bioresource Technology 99(7), 2507–2515.CrossRefGoogle ScholarPubMed
Golenda, C.F., Solberg, V.B., Burge, R., Gambel, J.M. & Wirtz, R.A. (1999) Gender-related efficacy difference to an extended duration formulation of topical N,N-diethyl-M-toluamide (DEET). American Journal of Tropical Medicine and Hygiene 60(4), 654–657.CrossRefGoogle Scholar
Goodyer, L. & Song, J. (2014) Mosquito bite-avoidance attitudes and behaviors in travelers at risk of malaria. Journal of Travel Medicine 21(1), 33–38.CrossRefGoogle ScholarPubMed
Govere, J., Durrheim, D.N., Baker, L., Hunt, R. & Coetzee, M. (2000) Efficacy of three insect repellents against the malaria vector Anopheles arabiensis . Medical and Veterinary Entomology 14(4), 441–444.CrossRefGoogle ScholarPubMed
Gupta, R.K. & Rutledge, L.C. (1991) Controlled release repellent formulations on human volunteers under three climatic regimens. Journal of the American Mosquito Control Association 7(3), 490–493.Google ScholarPubMed
Gupta, R.K. & Rutledge, L.C. (1994) Role of repellents in vector control and disease prevention. American Journal of Tropical Medicine and Hygiene 50(6 Suppl), 82–86.CrossRefGoogle ScholarPubMed
Health Protection Agency (2007) Guidelines for Malaria Prevention in Travelers from the United Kingdom. London, Health Protection Agency.Google Scholar
Hidayatulfathi, O., Sallehuddin, S. & Ibrahim, J. (2004) Adulticidal activity of some Malaysian plant extracts against Aedes aegypti Linnaeus. Tropical Biomedicine 21(2), 61–67.Google ScholarPubMed
Katz, T.M., Miller, J.H. & Hebert, A.A. (2008) Insect repellents: historical perspectives and new developments. Journal of the American Academy of Dermatology 58(5), 865–871.CrossRefGoogle ScholarPubMed
Keziah, E.A., Nukenine, E.N., Danga, S.P., Younoussa, L. & Esimone, C.O. (2015) Creams formulated with Ocimum gratissimum L. and Lantana camara L. crude extracts and fractions as mosquito repellents against Aedes aegypti L. (Diptera: Culicidae). Journal of Insect Science 15, 45.CrossRefGoogle Scholar
Logan, J.G., Stanczyk, N.M., Hassanali, A., Kemei, J., Santana, A.E.G., Ribeiro, K.A.L., Pickett, J.A. & Mordue Luntz, A.J. (2010) Arm-in-cage testing of natural human-derived mosquito repellents. Malaria Journal 9, 239.CrossRefGoogle ScholarPubMed
Meepagala, K.M., Bernier, U.R., Burandt, C. & Duke, S.O. (2013) Mosquito repellents based on a natural chromene analogue with longer duration of action than N,N-diethyl-meta-toluamide (DEET). Journal of Agricultural and Food Chemistry 61(39), 9293–9297.CrossRefGoogle Scholar
Meltzer, E. & Schwartz, E. (2009) A travel medicine view of dengue and dengue hemorrhagic fever. Travel Medicine and Infectious Disease 7(5), 278–283.CrossRefGoogle ScholarPubMed
Misni, N., Sulaiman, S. & Othman, H. (2008) The repellent activity of Piper aduncum Linn (Family: Piperaceae) essential oil against Aedes aegypti using human volunteers. Journal of Tropical Medicine & Parasitology 31, 63–69.Google Scholar
Mukesh, Y., Savitri, P., Kaushik, R. & Singh, N.P. (2014) Studies on repellent activity of seed oils alone and in combination on mosquito, Aedes aegypti . Journal of Environmental Biology 35(5), 917–922.Google ScholarPubMed
Nerio, L.S., Olivero-Verbel, J. & Stashenko, E. (2010) Repellent activity of essential oils: a review. Bioresource Technology 101(1), 372–378.CrossRefGoogle ScholarPubMed
Novak, R.J. & Gerberg, E.J. (2005) Natural-based repellent products: efficacy for military and general public uses. Journal of the American Mosquito Control Association 21, 7–11.CrossRefGoogle ScholarPubMed
Olander, L.P., Scatena, F.N. & Silver, W.L. (1998) Impacts of disturbance initiated by road construction in a subtropical cloud forest in the Luquillo Experimental Forest, Puerto Rico. Forest Ecology and Management 109, 33–49.CrossRefGoogle Scholar
Omotosho, J.A., Whateley, T.L., Law, T.K. & Florence, A.T. (1986) The nature of the oil phase and the release of solutes from multiple (w/o/w) emulsions. Journal of Pharmacy and Pharmacology 38(12), 865–870.CrossRefGoogle ScholarPubMed
Osimitz, T.G. & Grothaus, R.H. (1995) The present safety assessment of DEET. Journal of the American Mosquito Control Association 11(2), 274–278.Google ScholarPubMed
Oyedele, A.O., Orafidiya, L.O., Lamikanra, A. & Olaifa, J.I. (2000) Volatility and mosquito repellency of Hemizygia welwitschii Rolfe oil and its formulations. International Journal of Tropical Insect Science 20(2), 123–128.CrossRefGoogle Scholar
Oyedele, A.O., Gbolade, A.A., Sosan, M.B., Adewoyin, F.B., Soyelu, O.L. & Orafidiya, O.O. (2002) Formulation of an effective mosquito-repellent topical product from lemongrass oil. Phytomedicine 9(3), 259–262.CrossRefGoogle ScholarPubMed
Phasomkusolsil, S. & Soonwera, M. (2010) Insect repellent activity of medicinal plant oils against Aedes aegypti (Linn.), Anopheles minimus (Theobald) and Culex quinquefasciatus Say based on protection time and biting rate. Southeast Asian Journal of Tropical Medicine and Public Health 41(4), 831–840.Google ScholarPubMed
Pohlit, A.M., Pinto, A.C.S. & Mause, R. (2006) Piper aduncum L.: pluripotente plant and important phytochemical substance source. Revista Fitos 2, 7–18.CrossRefGoogle Scholar
Rozendaal, J.A. (1997) Vector Control: Methods for use by Individuals and Communities. Geneva, World Health Organization.Google Scholar
Schwartz, E., Weld, L.H., Wilder-Smith, A., Von Sonnenburg, F., Keystone, J.S., Kain, K.C., Torresi, J. & Freedman, D.O. (2008) Seasonality, annual trends, and characteristics of dengue among ill returned travelers, 1997–2006. Emerging Infectious Diseases 14(7), 1081–1088.CrossRefGoogle ScholarPubMed
Sritabutra, D., Soonwera, M., Waltanachanobon, S. & Poungjai, S. (2011) Evaluation of herbal essential oil as repellents against Aedes aegypti (L.) and Anopheles dirus Peyton & Harrion. Asian Pacific Journal of Tropical Biomedicine 1(1), 124–128.CrossRefGoogle Scholar
Standard and Industrial Research Institute of Malaysia (2007) Household Insecticide Products-Personal Mosquito Repellent – Evaluation Method for Biological Efficacy (MS1497:2007), Malaysia. Available online at http://www.freestd.us/soft4/2003367.htm.Google Scholar
Stienlauf, S., Segal, G., Sidi, Y. & Schwartz, E. (2005) Epidemiology of travel-related hospitalization. Journal of Travel Medicine 12(3), 136–141.CrossRefGoogle ScholarPubMed
Thavara, U., Tawatsin, A. & Chompoosri, J. (2002) Phytochemicals as repellents against mosquitoes in Thailand. pp. 244–250 in Proceedings of International Conference on Biopesticide 3, 21–26 April 2002, Kuala Lumpur.Google Scholar
Vatandoost, H. & Hanafi-Bojd, A.A. (2008) Laboratory evaluation of 3 repellents against Anopheles stephensi in the Islamic Republic of Iran. Eastern Mediterranean Health Journal 14(2), 260–267.Google ScholarPubMed
Zhu, B.C.R., Henderson, G., Chen, F., Fei, H. & Laine, R.A. (2001) Evaluation of vetiver oil and seven insect-active essential oils against the Formosan subterranean termite. Journal of Chemical Ecology 27(8), 1617–1625.CrossRefGoogle ScholarPubMed