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The effect of using prebiotic and probiotic products on intestinal micro-flora of the honeybee (Apis mellifera carpatica)

Published online by Cambridge University Press:  30 March 2012

S. Pătruică*
Affiliation:
Banat University of Agricultural Sciences and Veterinary Medicine, Faculty of Animal Science and Biotechnologies, Department of Apiculture, 119, Calea Aradului, 300645, Timisoara, Romania
D. Mot
Affiliation:
Banat University of Agricultural Sciences and Veterinary Medicine, Faculty of Animal Science and Biotechnologies, Department of Apiculture, 119, Calea Aradului, 300645, Timisoara, Romania
*
*Author for correspondence Fax:00400256277110 E-mail: [email protected] or [email protected]

Abstract

Maintaining bee colonies in a healthy state throughout the year is one of the main concerns of apiculture researchers. The phenomenon of disappearance of bee colonies is determined by several factors, one of which is bee disease. Due to the organizational structure of the bee colony, disease transmission is rapid, especially through infected food or via the nurse worker bees that feed the brood bees of the colony concerned. The practice of stimulating the bee colonies in spring using sugar syrup feeds with added prebiotic products (lactic acid or acetic acid) and probiotics (Lactobacillus acidophilus LA-14 and Bifidobacterium lactis BI-04) by using an Enterobiotic product (Lactobacillus casei), marketed as Enterolactis Plus, for three weeks, resulted in a significant reduction of the total number of bacteria in the digestive tracts of the bees, compared with the control group. By contrast, intestinal colonization with beneficial bacteria contained in probiotics products administered to the bees was observed. This resulted in an improved health status and bio productive index of the bee colonies studied.

Type
Research Paper
Copyright
Copyright © Cambridge University Press 2012

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References

Anderson, K.E., Sheehan, T.H., Eckholm, B.J., Mott, B.M. & Hoffman-DeGrandi, G. (2011) An emerging paradigm of colony health: microbial balance of the honey bee and hive (Apis mellifera). Insectes Sociaux 58(4), 431444, DOI:10.1007/s00040-011-0194-6.CrossRefGoogle Scholar
Bura, M. & Pătruică, S. (2003) Nutriţia şi Alimentaţia Albinelor. Timisoara, Romania, Agroprint Press.Google Scholar
Corcionivoschi, N. & Drinceanu, D. (2009) Probioticele la Present. Timisoara, Romania, Mirton Press.Google Scholar
Evens, J. & Lopez, D. (2004) Bacterial probiotics induce an immune response in the honey bee. Journal of Economic Entomology 97, 752756.Google Scholar
Feigenbaum, C. & Naug, D. (2010) The influence of social hunger on food distribution and its implication for disease transmission in a honeybee colony. Insectes Sociaux 57, 217222, DOI:10.1007/s00040-010-0073-6.Google Scholar
Forsgren, E., Olofsson, C.T., Vasquez, A. & Fries, I. (2009) Novel lactic acid bacteria inhibiting Paenibacillus larvae in honey bee larvae. Apidologie 41, 99108.Google Scholar
Fuselli, R.S., Garcia, R., Susana, B., Eguaras, J.M. & Fritz, R.Â. (2008) Chemical composition and antimicrobial activity of Citrus essences on honeybee bacterial pathogen Paenibacillus larvae the causal agent of American foulbrood. World Journal Microbiology Biotechnology 24, 20672072.CrossRefGoogle Scholar
Genersch, E. (2010) Honey bee pathology: current threats to honey bees and beekeeping. Applied Microbiology and Biotechnology 87 (1), 8797, DOI:10.1007/s00253-010-2573-8.Google Scholar
Hamdi, C., Balloi, A., Essanaa, J., Crotti, E., Gonella, E., Raddadi, N., Ricci, I., Boudabous, A., Borin, S., Manino, A., Bandi, C., Alma, A., Daffonchio, D. & Cherif, A. (2011) Gut microbiote dysbiosis and honeybee health. Journal of Applied Entomology 135(7), 524533.Google Scholar
Kacániová, M., Chlebo, R., Kopernický, M. & Trakovická, A. (2004) Microflora of honeybee gastrointestinal tract. Folia Microbiologica (Praha) 49(2), 167171.Google Scholar
Kacániová, M., Pavlicová, S., Hascik, P., Kociubinski, G., Kazovická, V., Sudzina, M., Sudzinová, J. & Fikselová, M. (2009) Microbial communities in bees, pollen and honey from Slovakia. Acta Microbiologica and Immunologica Hungarica 56(3), 285295.CrossRefGoogle ScholarPubMed
Koch, H. & Schmid-Hempel, P. (2011) Socially transmitted gut microbiota protect bumble bees against an intestinal parasite. Proceeding of the National Acaddemy of Sciences USA 108(48), 1928819292.CrossRefGoogle ScholarPubMed
Olofsson, T. & Vasquez, A. (2008) Detection and identification of a novel lactic and bacterial flora witin the honey stomach of the honeybee Apis mellifera. Current Microbiology 57, 356363.CrossRefGoogle Scholar
Martinson, V.G., Danforth, B.N., Minckley, R.L., Rueppell, O., Tingek, S. & Moran, N.A. (2011) A simple and distinctive microbiota associated with honey bees and bumble bees. Molecular Ecolology 20(3), 285295.Google Scholar
Moritz, R.F., Miranda, J., Fries, I., Conte, Y., Neuman, N. & Paxon, R.J. (2010) Research strategies to improve honeybee health in Europe. Apidologie 41(3), 227242, DOI:10.1051/apido/2010021.Google Scholar
Pătruică, S., Bura, M., Bănătean Dunea, I., Popescu, I., Simiz, E. & Schiopescu, P. (2006) Research on the influence of some apiary biostimulators on the development of bee colonies salping in the autumn season. Scientific Papers Animal Science and Biotechnologies, Timisoara, Romania 39, 117123.Google Scholar
Pătruică, S., Bogdan, A.T. & Bura, M. (2011a) The use prebiotic and probiotic products in the feeding bees. Agrobuletin AGIR 2(9), 118124.Google Scholar
Pătruică, S., Bogdan, A.T., Bura, M., Bănătean Dunea, I. & Gâltofet, M. (2011b) Research on the effect of acidifying substances on bee colonies development and health in spring. Scientific Papers Animal Science and Biotechnologies, Timisoara, Romania 44, 117123.Google Scholar
Pătruică, S., Bogdan, A.T., Bura, M. & Popovici, D. (2011c) Research on the effect of acidifying substances on bee colonies development and health in spring (2). Agrobuletin AGIR 2(9), 124130.Google Scholar
Pătruică, S., Bogdan, A.T., Bura, M. & Popovici, D. (2011d) Research on the influence of probiotic products on bee colonies development in spring. Bulletin UASVMCJ Animal Science and Biotechnologies 68, 451456.Google Scholar
Pătruică, S., Bogdan, A.T., Bura, M. & Popovici, D. (2011e) Evaluating the complementary effect of some prebiotic and probiotic products on the development of bee colonies during spring. Bulletin UASVMCJ Animal Science and Biotechnologies 68, 457458.Google Scholar
Porrini, M.P., Audisio, M.C., Sabaté, D.C., Ibarguren, C., Medici, S.K., Sarlo, E.G., Garrido, P.M. & Eguaras, M.J. (2010) Effect of bacterial metabolites on microsporidian Nosema ceranae and on its host Apis mellifera. Parasitology Research 107(2), 381388.Google Scholar
Soroker, V., Hetzroni, A., Yakobson, B., David, D., David, A., Voet, H., Slabezki, Y., Efrat, H., Levski, S., Kamer, Y., Klinberg, E., Zioni, N., Inbar, S. & Chejanovsky, N. (2010) Evaluation of colony losses in Israel in relation to incidence of pathogens and pests. Apidologie 42(2), 192199.Google Scholar
Stabentheiner, A., Vollmann, J., Kovac, H. & Crailsheim, K. (2003) Oxygen consumption and body temperature of active and resting honeybees. Journal of Insect Physiology 49(9), 881889.Google Scholar
Stoica, I., Stoica, L. & Pană, C. (1999) Aditivi furajeri. Bucharest, Romania, Coral Sanivet Press.Google Scholar
Stef, L. (2003) Tehnologia Producerii Nutreturilor Combinate. Timisoara, Romania, Waldpress Press.Google Scholar
Vandame, R. & Palacio, M.A. (2010) Preserved honey bee health in Latin America: a fragile equilibrum due to low intensity agriculture and beekeeping. Apidologie 41(2), 243255,CrossRefGoogle Scholar