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Determination of the efficiency of diets for larval development in mass rearing Aedes aegypti (Diptera: Culicidae)

Published online by Cambridge University Press:  23 November 2017

P.A.D.H.N. Gunathilaka ,
U.M.H.U. Uduwawala ,
N.W.B.A.L. Udayanga ,
R.M.T.B. Ranathunge ,
L.D. Amarasinghe  and
W. Abeyewickreme
P.A.D.H.N. Gunathilaka*
Affiliation:
Department of Parasitology, Faculty of Medicine, University of Kelaniya, Sri Lanka
U.M.H.U. Uduwawala
Affiliation:
Department of Zoology and Environment Management, Faculty of Science, University of Kelaniya, Sri Lanka
N.W.B.A.L. Udayanga
Affiliation:
Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Sri Lanka
R.M.T.B. Ranathunge
Affiliation:
Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Sri Lanka
L.D. Amarasinghe
Affiliation:
Department of Zoology and Environment Management, Faculty of Science, University of Kelaniya, Sri Lanka
W. Abeyewickreme
Affiliation:
Department of Parasitology, Faculty of Medicine, University of Kelaniya, Sri Lanka Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Sri Lanka
*
*Author for correspondence Tel: +94 11 2958039 Fax: +94 11 2958337 E-mail: [email protected]
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Abstract

Larval diet quality and rearing conditions have a direct and irreversible effect on adult traits. Therefore, the current study was carried out to optimize the larval diet for mass rearing of Aedes aegypti, for Sterile Insect Technique (SIT)-based applications in Sri Lanka. Five batches of 750 first instar larvae (L1) of Ae. aegypti were exposed to five different concentrations (2–10%) of International Atomic Energy Agency (IAEA) recommended the larval diet. Morphological development parameters of larva, pupa, and adult were detected at 24 h intervals along with selected growth parameters. Each experiment was replicated five times. General Linear Modeling along with Pearson's correlation analysis were used for statistical treatments. Significant differences (P < 0.05) among the larvae treated with different concentrations were found using General Linear Modeling in all the stages namely: total body length and the thoracic length of larvae; cephalothoracic length and width of pupae; thoracic length, thoracic width, abdominal length and the wing length of adults; along with pupation rate and success, sex ratio, adult success, fecundity and hatching rate of Ae. aegypti. The best quality adults can be produced at larval diet concentration of 10%. However, the 8% larval diet concentration was most suitable for adult male survival.

Keywords

Aedesdietoptimizationdevelopment
Type
Research Papers
Information
Bulletin of Entomological Research , Volume 108 , Issue 5 , October 2018 , pp. 583 - 592
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Copyright
Copyright © Cambridge University Press 2017 

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