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Cryptic species within Anopheles longipalpis from southern Africa and phylogenetic comparison with members of the An. funestus group

Published online by Cambridge University Press:  21 August 2008

L.L. Koekemoer ,
E.A. Misiani ,
R.H. Hunt ,
R.J. Kent ,
D.E. Norris  and
M. Coetzee
L.L. Koekemoer*
Affiliation:
Vector Control Reference Unit, National Institute for Communicable Diseases, NHLS, Private bag X 4, Sandringham, 2131, South Africa Division of Virology and Communicable Disease Surveillance, School of Pathology of the National Health Laboratory Service and the University of the Witwatersrand, Johannesburg, South Africa
E.A. Misiani
Affiliation:
Vector Control Reference Unit, National Institute for Communicable Diseases, NHLS, Private bag X 4, Sandringham, 2131, South Africa Division of Virology and Communicable Disease Surveillance, School of Pathology of the National Health Laboratory Service and the University of the Witwatersrand, Johannesburg, South Africa
R.H. Hunt
Affiliation:
Vector Control Reference Unit, National Institute for Communicable Diseases, NHLS, Private bag X 4, Sandringham, 2131, South Africa School of Animal, Plant & Environmental Sciences, University of the Witwatersrand, Johannesburg, South Africa
R.J. Kent
Affiliation:
The W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA
D.E. Norris
Affiliation:
The W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA
M. Coetzee
Affiliation:
Vector Control Reference Unit, National Institute for Communicable Diseases, NHLS, Private bag X 4, Sandringham, 2131, South Africa Division of Virology and Communicable Disease Surveillance, School of Pathology of the National Health Laboratory Service and the University of the Witwatersrand, Johannesburg, South Africa
*
*Author for correspondence Fax: +27-11-386 6481 E-mail: [email protected]
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Abstract

House-resting Anopheles mosquitoes are targeted for vector control interventions; however, without proper species identification, the importance of these Anopheles to malaria transmission is unknown. Anopheles longipalpis, a non-vector species, has been found in significant numbers resting indoors in houses in southern Zambia, potentially impacting on the utilization of scarce resources for vector control. The identification of An. longipalpis is currently based on classical morphology using minor characteristics in the adult stage and major ones in the larval stage. The close similarity to the major malaria vector An. funestus led to investigations into the development of a molecular assay for identification of An. longipalpis. Molecular analysis of An. longipalpis from South Africa and Zambia revealed marked differences in size and nucleotide sequence in the second internal transcribed spacer (ITS2) region of ribosomal DNA between these two populations, leading to the conclusion that more than one species was being analysed. Phylogenetic analysis showed the Zambian samples aligned with An. funestus, An. vaneedeni and An. parensis, whereas the South African sample aligned with An. leesoni, a species that is considered to be more closely related to the Asian An. minimus subgroup than to the African An. funestus subgroup. Species-specific primers were designed to be used in a multiplex PCR assay to distinguish between these two cryptic species and members of the An. funestus subgroup for which there is already a multiplex PCR assay.

Keywords

Anopheles longipalpisAnopheles funestusSouth AfricaZambiainternal transcribed spacer (ITS2)PCRphylogenetics
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 99 , Issue 1 , February 2009 , pp. 41 - 49
Copyright
Copyright © 2008 Cambridge University Press

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References

Chen, B., Butlin, R.K., Pedro, P.M., Wang, X.Z. & Harbach, R.E. (2006) Molecular variation, systematics and distribution of the Anopheles fluviatilis complex in southern Asia. Medical and Veterinary Entomology 20, 33–43.CrossRefGoogle ScholarPubMed
Cohuet, A., Simard, F., Toto, J., Kengne, P., Coetzee, M. & Fontenille, D. (2003) Species identification within the Anopheles funestus group of malaria vectors in Cameroon and evidence for a new species. American Journal of Tropical Medicine and Hygiene 69, 200–205.CrossRefGoogle ScholarPubMed
Collins, F.H., Mendez, M.A., Rasmussen, M.O., Meheffey, P.C., Besansky, N.J. & Finnerty, V. (1987) A ribosomal RNA gene probe differentiates member species of the Anopheles gambiae complex. American Journal of Tropical Medicine and Hygiene 37, 37–41.CrossRefGoogle ScholarPubMed
Corpet, F. (1988) Multiple sequence alignment with hierarchical clustering. Nucleic Acid Research 16, 10881–10890.CrossRefGoogle ScholarPubMed
Felsenstein, J. (1985) Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39, 783–791.CrossRefGoogle ScholarPubMed
Garros, C., Harbach, R.E. & Manguin, S. (2005a) Morphological assessment and molecular phylogenetics of the Funestus and Minimus Groups of Anopheles (Cellia). Journal of Medical Entomology 42, 521–536.Google ScholarPubMed
Garros, C., Harbach, R.E. & Manguin, S. (2005b) Systematics and Biogeographical implications of the phylogenetic relationships between members of the Funestus and Minimus Groups of Anopheles (Diptera: Culicidae). Journal of Medical Entomology 42, 7–18.CrossRefGoogle ScholarPubMed
Gillies, M.T. & Coetzee, M. (1987) A Supplement to the Anophelineof Africa South of the Sahara. pp. 1–139. Johannesburg, South African Medical research Institute for Medical Research, Publication, No. 55.Google Scholar
Gillies, M.T. & De Meillon, B. (1968) The Anophelinae of Africa, South of the Sahara. pp. 1–343. Johannesburg, South African Institute of Medical Research, No. 55.Google Scholar
Green, C.A. (1982) Cladistic analysis of mosquito chromosome data: Anopheles (Cellia) Myzomyia. Journal of Heredity 73, 2–11.CrossRefGoogle ScholarPubMed
Hackett, B.J., Gimnig, J., Guelbeogo, W., Costantini, C., Koekemoer, L.L., Coetzee, M., Collins, F.H. & Besansky, N.J. (2000) Ribosomal DNA internal transcribed specer (ITS2) sequences differentiate Anopheles funestus and An. rivulorum, and uncover a cryptic taxon. Insect Molecular Biology 9, 369–374.CrossRefGoogle Scholar
Harbach, R.E. (2004) The classification of genus Anopheles (Diptera: Culicidae): a working hypothesis of phylogenetic relationships. Bulletin of Entomological Research 94, 537–553.CrossRefGoogle ScholarPubMed
Harbach, R.E., Parkin, E., Chen, B. & Butlin, R.K. (2006) Anopheles (Cellia) minimus Theobald (Dipetera: Culicidae): neotype designation, characterization, and systematics. Proceedings of the Entomological Society of Washington 108, 198–209.Google Scholar
Harbach, R.E., Garros, C., Manh, N.D. & Manguin, S. (2007) Formal taxonomy of species C of the Anopheles minimus sibling species complex (Diptera: Culicidae) Zootaxa 1654, 41–54.CrossRefGoogle Scholar
Hargreaves, K., Koekemoer, L.L., Brooke, B.D., Hunt, R.H., Mthembu, J. & Coetzee, M. (2000) Anopheles funestus resistant to pyrethroid insecticides in South Africa. Medical and Veterinary Entomology 14, 181–189.CrossRefGoogle ScholarPubMed
Hunt, R.H., Brooke, B.D., Pillay, C., Koekemoer, L.L. & Coetzee, M. (2005) Laboratory selection for and characteristics of pyrethroid resistance in the malaria vector Anopheles funestus. Medical and Veterinary Entomology 17, 417–422.Google Scholar
Kent, R.J., Coetzee, M., Mharakurwa, S. & Norris, D.E. (2006) Feeding and indoor resting behaviour of the mosquito Anopheles longipalpis in an area of hyperendemic malaria transmission in southern Zambia. Medical and Veterinary Entomology 20, 459–463.CrossRefGoogle Scholar
Koekemoer, L.L., Kamau, L., Hunt, R.H. & Coetzee, M. (2002)A cocktail polymerase chain reaction assay to identify members of the Anopheles funestus (Diptera: Culicidae) group. American Journal of Tropical Medicine and Hygiene 66, 804–811.CrossRefGoogle ScholarPubMed
Mukayabire, O., Boccolini, D., Lochouarn, L., Fontenille, D. & Besansky, N.J. (1999) Mitochondrial and ribosomal internal transcribed spacer (ITS2) diversity of the African malaria vector Anopheles funestus. Molecular Ecology 8, 289–297.CrossRefGoogle Scholar
Paskewitz, S.M., Wesson, D.M. & Collins, F.H. (1993) The internal transcribed spacers of ribosomal DNA in five members of the Anopheles gambiae species complex. Insect Molecular Biology 2, 247–257.CrossRefGoogle ScholarPubMed
Swofford, D. (2003) PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods), Ver. 4. Sunderland, MA, USA, Sinauer Associates.Google Scholar
Theobald, F.V. (1903) A Monograph of the Culicidae or Mosquitoes. London, The British Natural History Museum.Google Scholar
White, G.B. (1972) Confirmation that Anopheles longipalpis (Theobald) and Anopheles confusus Evans and Leeson occur in Ethiopia. Mosquito Systematics 4, 131–132.Google Scholar